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serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1219 [new locus tag: SPJ_RS06065 ]
  • pan locus tag?: PNEUPAN002639000
  • symbol: SPJ_1219
  • pan gene symbol?: gdhA
  • synonym:
  • product: NAD(p)-specific glutamate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1219 [new locus tag: SPJ_RS06065 ]
  • symbol: SPJ_1219
  • product: NAD(p)-specific glutamate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1172643..1173989
  • length: 1347
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACATCTGCTAAAGAATATATCCAAAGCGTGTTTGAAACTGTGAAAGCTCGTAACGGG
    CACGAGGCTGAATTCCTCCAAGCTGTTGAAGAATTTTTCAACACTTTAGAACCTGTATTT
    GAAAAACACCCTGAGTATATCGAAGAAAATATCTTGGCACGTATTACTGAGCCTGAGCGC
    GTGGTTTCTTTCCGTGTTCCTTGGGTTGACCGTGATGGAAAAATTCAAGTAAACCGTGGT
    TACCGTGTTCAATTCAACTCAGCTGTTGGACCATACAAAGGTGGACTTCGTTTCCACCCA
    ACTGTAAACCAAGGGATCTTGAAATTCCTCGGATTTGAACAAATCTTTAAAAATGTCTTG
    ACTGGACTTCCTATCGGTGGAGGTAAAGGTGGATCAGACTTCGATCCTAAAGGTAAAACA
    GATGCTGAAGTGATGCGCTTCTGCCAAAGCTTCATGACTGAATTGCAAAAACACATCGGA
    CCATCACTTGACGTACCTGCTGGTGATATCGGTGTTGGTGGACGTGAAATTGGTTACCTT
    TACGGTCAATACAAACGTCTTAACCAATTTGATGCTGGTGTCTTGACTGGTAAACCTCTT
    GGATTTGGTGGTAGCTTGATTCGTCCAGAAGCAACTGGTTACGGTTTGGTTTACTATACT
    GAAGAAATGCTCAAAGCTAACGGTAACAGCTTTGCTGGTAAGAAAGTCGTTATTTCAGGT
    TCTGGTAACGTTGCTCAATATGCTCTTCAAAAAGCAACTGAACTCGGTGCAACTGTTATT
    TCTGTTTCTGACTCAAATGGTTATGTCATCGATGAAAATGGTATCGACTTCGATCTTTTG
    GTTGATGTTAAAGAAAAACGTCGTGCTCGTTTGACTGAGTATGCAGCTGAAAAAGCAACT
    GCAACCTACCACGAAGGTACTGTATGGACTTACGCTGGAAACTATGACATTGCTCTTCCA
    TGTGCCACTCAAAACGAAATCAACGGTGAAGCAGCTAAACGTTTGGTTGCTCAAGGCGTT
    ATCTGTGTATCTGAAGGTGCCAACATGCCGAGCGACCTTGATGCCATCAAAGTTTACAAA
    GAAAATGGTATCTTCTACGGACCTGCAAAAGCTGCCAACGCTGGTGGTGTAGCCGTTTCA
    GCTCTTGAAATGAGCCAAAACAGTCTTCGCCTCTCATGGACTCGTGAAGAAGTTGATGGC
    CGTCTCAAAGACATCATGACAAACATCTTCAACACAGCTAAAACGACTTCAGAAACATAC
    GGTCTTGATAAAGACTACCTTGCAGGAGCTAACATTGCTGCCTTTGAAAATGTAGCAAAT
    GCCATGATTGCACAAGGTATTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1219 [new locus tag: SPJ_RS06065 ]
  • symbol: SPJ_1219
  • description: NAD(p)-specific glutamate dehydrogenase
  • length: 448
  • theoretical pI: 5.12156
  • theoretical MW: 48810.8
  • GRAVY: -0.233259

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.3?  ExPASy
    Glutamate dehydrogenase (NAD(P)+) L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H
  • TIGRFAM:
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 12.2)
  • TheSEED  :
    • NADP-specific glutamate dehydrogenase (EC 1.4.1.4)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  NADP-specific glutamate dehydrogenase (EC 1.4.1.4)
    and 3 more
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamate dehydrogenases  NADP-specific glutamate dehydrogenase (EC 1.4.1.4)
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  NADP-specific glutamate dehydrogenase (EC 1.4.1.4)
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline Synthesis  NADP-specific glutamate dehydrogenase (EC 1.4.1.4)
  • PFAM:
    NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 296.6)
    and 4 more
    AA_dh_N (CL0603) ELFV_dehydrog_N; Glu/Leu/Phe/Val dehydrogenase, dimerisation domain (PF02812; HMM-score: 168.4)
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 19.5)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 14.2)
    no clan defined AglB_L1; Archaeal glycosylation protein B long peripheral domain (PF18079; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003128
    • TAT(Tat/SPI): 0.000251
    • LIPO(Sec/SPII): 0.000461
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GlnR* (repression) regulon, CodY (activation (repression)) regulon
    GlnR*(TF)important in Nitrogen assimilation; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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