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serotype 4
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D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1260 [new locus tag: SPJ_RS06260 ]
  • pan locus tag?: PNEUPAN002685000
  • symbol: thrB
  • pan gene symbol?: thrB
  • synonym:
  • product: homoserine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1260 [new locus tag: SPJ_RS06260 ]
  • symbol: thrB
  • product: homoserine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1206428..1207297
  • length: 870
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAGATTATTGTACCTGCAACCAGTGCCAATATCGGGCCAGGTTTTGACTCGGTCGGT
    GTAGCTGTAACCAAGTATCTTCAAATTGAGGTCTGCGAAGAACGAGATGAGTGGCTGATT
    GAACACCAGATTGGCAAATGGATTCCACATGATGAGCGTAATCTCTTGCTCAAAATCGCT
    TTGCAAATTGTACCAGACTTGCAACCAAGACGCTTGAAAATGACCAGTGATGTCCCTTTG
    GCGCGCGGTTTGGGTTCTTCCAGCTCGGTTATCGTTGCTGGGATTGAACTAGCCAACCAA
    CTGGGTCAACTCAACTTATCAGACCATGAAAAATTGCAGTTAGCGACCAAGATTGAAGGG
    CATCCTGACAATGTGGCTCCAGCCATTTATGGTAATCTCGTTATTGCAAGTTCTGTTGAA
    GGGCAAGTCTCTGCTATCGTAGCAGACTTTCCAGAGTGTGATTTTCTAGCTTACATTCCA
    AACTATGAATTACGTACTCGCGACAGCCGTAGTGTCTTGCCTAAAAAATTGTCTTATAAG
    GAAGCTGTTGCTGCAAGTTCTATCGCCAATGTAGCGGTTGCTGCCTTGTTGGCAGGAGAC
    ATGGTGACCGCTGGGCAAGCAATCGAGGGAGACCTCTTCCATGAGCGTTATCGTCAGGAC
    TTGGTAAGAGAATTTGCGATGATTAAGCAAGTGACCAAAGAAAATGGGGCCTATGCAACC
    TACCTTTCTGGTGCTGGGCCGACAGTTATGGTTCTGGCTTCTCATGACAAGATGCCAACA
    ATTAAGGCAGAATTGGAAAAGCAACCTTTCAAAGGAAAACTGCATGACTTGAGAGTTGAT
    ACCCAAGGTGTCCGTGTAGAAGCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1260 [new locus tag: SPJ_RS06260 ]
  • symbol: ThrB
  • description: homoserine kinase
  • length: 289
  • theoretical pI: 6.17284
  • theoretical MW: 31548.1
  • GRAVY: -0.0560554

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.39?  ExPASy
    Homoserine kinase ATP + L-homoserine = ADP + O-phospho-L-homoserine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 245.3)
    and 6 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 35.3)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 29.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 28.6)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 17.5)
    Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 15.8)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 15.7)
  • TheSEED  :
    • Homoserine kinase (EC 2.7.1.39)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Homoserine kinase (EC 2.7.1.39)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Homoserine kinase (EC 2.7.1.39)
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 50.9)
    and 2 more
    GHMP_C (CL0677) GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 29.5)
    no clan defined Ferredoxin_N; N-terminal region of 4Fe-4S ferredoxin iron-sulfur binding (PF16947; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005692
    • TAT(Tat/SPI): 0.000251
    • LIPO(Sec/SPII): 0.000799
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQIGKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLGQLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIPNYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYRQDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHDLRVDTQGVRVEAK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (activation (repression)) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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