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JJA
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MDRSPN001
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NCTC7466
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NU83127
serotype 4
OXC141
serotype 3
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SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
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SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
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TCH8431/19A
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Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1788 [new locus tag: SPJ_RS08890 ]
  • pan locus tag?: PNEUPAN003376000
  • symbol: treC
  • pan gene symbol?: treC
  • synonym:
  • product: alpha,alpha-phosphotrehalase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1788 [new locus tag: SPJ_RS08890 ]
  • symbol: treC
  • product: alpha,alpha-phosphotrehalase
  • replicon: chromosome
  • strand: -
  • coordinates: 1704251..1705876
  • length: 1626
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACACTTGATAAAGGAAAAGTAGTTTATCAAATCTATCCAAAATCTTACAAAGACACC
    ACTGAAAACGGTATTGGGGATTTCCGTGGGATTATCGAAAAAATTCCCTATTTAGCCAAA
    CTAGGTGTGGATATGGTCTGGCTCAATCCATTCTATCCAAGCCCTCAACGGGATAATGGT
    TACGATATTTCAGATTATATGGCAGTGGATCCTCTTTTTGGTGATATGGCTGATTTTGAG
    GAAATGGTATGTGTCGGTAAAGAGCACAAGATTGACTTTATGCTGGACATGGTACTTAAT
    CATTGTTCGACAGAGCATGAATGGTTTCAGAAAGCCCTAGCTGGTGATAAGTATTATCAA
    GACTTTTTCTTCATCCAAGACCAACCAACAGATTGGCAGTCTAAGTTTGGTGGTTCTGCA
    TGGGCGCCTTTCGGGGATACTGGGAAATACTATCTTCACCTATTTGATGAGACCCAGGCT
    GACCTTAACTGGCGCAATCCCAATGTCCGTAAGGAGCTTTTCAAGGTTGTTAATTTCTGG
    CGCGACAAGGGTGTCAAAGGTTTTCGATTTGATGTGATCAATTTGATCGGTAAGGACGAG
    GTTTCAGTGGACTGTCCTGAAAATGAAGGGAAGCCAGCTTATACAGACAAACCCATCGTT
    CATAACTATTTGCGTATGATGAACCAAGCCACTTTTGGATCTGACGATAGCTTTATGACA
    GTTGGGGAAATGTCTTCTACCACCATGGAAAACTGTGTCCTTTATTCGTCGCCTGATCGT
    CAGGAATTATCCATGACCTTTAATTTTCATCACCTCAAGGTGGACTATAAGGATGGACAA
    AAGTGGACCTTGGCTCCCTTTGATTTTGAAGAGTTGAAGAGTCTTTACCATAGTTGGGGT
    AAGGAAATGAGTGATAAAGACGGTTGGAGTGCCCTCTTTTGGAACAATCACGATCAACCA
    CGTGCCTTGAACCGTTTTGTCGATATTCAAAACTTCCGCAAGGAAGGAGCCACCATGCTA
    GCAGCTAGCATTCACCTGTCACGTGGGACACCTTATATCTACATGGGTGAGGAAATCGGG
    ATGATTGACCCAGACTATGATTCTATGGCTGATTATGTGGATGTCGAATCGCTTAATGCC
    TATCAGATGCTCTTAGAAGAAGGAAAGAGTCAGCAAGAAGCCTTCCAGATTATTCAAGCT
    AAGTCGCGTGATAATTCACGCATTCCCATGCAGTGGGATGCTTCGGAAAATGCAGGATTT
    TCAACAGGCACTCCTTGGTTGAAAGCTGGTAAATCCTATAAAGATATCAATGTAGAAAAT
    GAAATCAAAGGTCTGATTTTCACCTTCTATCAAGACTTGATTCGACTGCGTAAGGAAATG
    CCAATCATTTCAGAAGGAAATTACAAACCAGCCTTTGAAGATAGCAAACAAGTCTACGCT
    TTTGAACGCCAGTTTGAGGATCAAAAGTTACTAGTGCTCAATAACTTTTATGCCAAAGAA
    GTGGAAATCGACTTGCCAGCAGTCTACCAAAATGGACAGATTTTGATTTCAAACTATGAA
    GATGCAGAAGTGTCTGAGAAAATTCTACTCAAACCCTATCAAACACTGGCTATTTATGTA
    AATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1626

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1788 [new locus tag: SPJ_RS08890 ]
  • symbol: TreC
  • description: alpha,alpha-phosphotrehalase
  • length: 541
  • theoretical pI: 4.53634
  • theoretical MW: 62822.4
  • GRAVY: -0.536969

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.93?  ExPASy
    Alpha,alpha-phosphotrehalase Alpha,alpha-trehalose 6-phosphate + H2O = D-glucose + D-glucose 6-phosphate
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 847.4)
    and 5 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 247.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 60)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 51.7)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 34.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 28.1)
  • TheSEED  :
    • Trehalose-6-phosphate hydrolase (EC 3.2.1.93)
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  Trehalose-6-phosphate hydrolase (EC 3.2.1.93)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 354.9)
    and 4 more
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 31.8)
    Glyco_hydro_tim (CL0058) Glyco_hydro_77; 4-alpha-glucanotransferase (PF02446; HMM-score: 20.9)
    GHD (CL0369) DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 20)
    Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 18.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00309
    • TAT(Tat/SPI): 0.00014
    • LIPO(Sec/SPII): 0.000372
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVDMVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHCSTEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQADLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKPIVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDYKDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRKEGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQEAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIKGLIFTFYQDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAVYQNGQILISNYEDAEVSEKILLKPYQTLAIYVN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, TreR (repression) regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    TreR(TF)important in Trehalose utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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