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70585
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Hu15
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INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
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NU83127
serotype 4
OXC141
serotype 3
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R6
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SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
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SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_2194 [new locus tag: SPJ_RS10875 ]
  • pan locus tag?: PNEUPAN003873000
  • symbol: SPJ_2194
  • pan gene symbol?: fucR
  • synonym:
  • product: regulator of fucose operon

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_2194 [new locus tag: SPJ_RS10875 ]
  • symbol: SPJ_2194
  • product: regulator of fucose operon
  • replicon: chromosome
  • strand: +
  • coordinates: 2046120..2046893
  • length: 774
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATAAAAGATGAACGTGTACTTGAATTGATTGAAATTATTAAAAAGAAAAAAAGAATT
    GCCGTAAAAGAGCTGGCAGAAATCACTTTCTCCAGCACAAGTACCTTACGTCGTGATTTA
    ATTTTCTTAGAAAATCAAGGTCTTATCAAAAGAAAGCACGGATACGTGACCCTGTCCTCT
    ATGAACACAATTGAACTTTCTCATCAAATACGGGAAGGAGAAAGTACTAGGCAAAAAAGA
    CTAATCGCTAGTCTCGCTAAAGACTTTATTCGGTCTGGTATGTGTGTTTATCTGGATTCC
    AGTACTACTGTCTACGAACTTTGTCCCTATCTTTCTGAACTTGATAATTTGATTATTTTT
    ACAAATGGTTTACATACTGCACAAACCCTATCTGAAACTGTTAAAGATAGCTCCAAAATC
    TTTATCACATCTGGCGAGGTCAAACATCAATCCTGTTCCGTGGTCAACTATGATAAGGAA
    AATTCTTTATTAGATCATTTTAATATCGATTTAGCATTTTGTTCAGCAAGAGGTATTGAT
    GACCAATATGTTTATGAAGCTTCTCTCAGCCAAGCTATTTCAAAAAAGAATATTATTGAC
    AAAGCCCATGAAACCATCTTACTGATTGATAGTTCTAAATTTTACAAGACTGGATTTTTT
    AAAATTAATCCCCTCTCCAAATACACAACCTTTATTTCAGACACCGTGCCAGATCAAAAA
    TTATTAGACGCAGTCGAATTATTTGATGGAGAATGGGTTTCTGATATTCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    774

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_2194 [new locus tag: SPJ_RS10875 ]
  • symbol: SPJ_2194
  • description: regulator of fucose operon
  • length: 257
  • theoretical pI: 6.25546
  • theoretical MW: 29269.3
  • GRAVY: -0.186381

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 20.6)
    and 1 more
    Signal transduction Regulatory functions DNA interactions beta-ketoadipate pathway transcriptional regulators, PcaR/PcaU/PobR family (TIGR02431; HMM-score: 13.3)
  • TheSEED  :
    • Predicted transcriptional regulator for fucose utilization, DeoR family
    Carbohydrates Monosaccharides L-fucose utilization  L-fucose operon activator
  • PFAM:
    ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 150.5)
    and 11 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 44.3)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.3)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 18.6)
    HTH_11; HTH domain (PF08279; HMM-score: 17.5)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 15.8)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 14.8)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 14.5)
    MarR; MarR family (PF01047; HMM-score: 12.8)
    LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 12.6)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.2)
    HTH_PafC; PafC helix-turn-helix domain (PF19187; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003261
    • TAT(Tat/SPI): 0.000512
    • LIPO(Sec/SPII): 0.000367
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLENQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSSTTVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKENSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTGFFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: FucR* (repression) regulon, CcpA regulon
    FucR*(TF)important in Fucose utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Pan F Chan, Karen M O'Dwyer, Leslie M Palmer, Jennifer D Ambrad, Karen A Ingraham, Chi So, Michael A Lonetto, Sanjoy Biswas, Martin Rosenberg, David J Holmes, Magdalena Zalacain
Characterization of a novel fucose-regulated promoter (PfcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae.
J Bacteriol: 2003, 185(6);2051-8
[PubMed:12618474] [WorldCat.org] [DOI] (P p)Irfan Manzoor, Sulman Shafeeq, Muhammad Afzal, Oscar P Kuipers
Fucose-Mediated Transcriptional Activation of the fcs Operon by FcsR in Streptococcus pneumoniae.
J Mol Microbiol Biotechnol: 2015, 25(2-3);120-8
[PubMed:26159073] [WorldCat.org] [DOI] (I p)

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