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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 10-APR-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN034156
  • locus tag: SPN034156_RS08850 [old locus tag: SPN034156_15920 ]
  • pan locus tag?: PNEUPAN001487000
  • symbol: cysS
  • pan gene symbol?: cysS
  • synonym:
  • product: cysteine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN034156_RS08850 [old locus tag: SPN034156_15920 ]
  • symbol: cysS
  • product: cysteine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1643969..1645312
  • length: 1344
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_021006 (1643969..1645312) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0515 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATTAAAATCTATGACACCATGTCTCGTGATTTGCGAGAATTTGTCCCGATTGAGGAC
    GGCAAGATCAAGATGTATGTTTGTGGGCCAACGGTGTACAACTATATCCACGTGGGAAAC
    GCCCGTTCGACGGTAGCTTTTGATACGATTCGTCGCTATTTTGAGTACCGTGGGTATAAG
    GTTGCCTATATTTCCAATTTTACAGATGTGGATGATAAGATTATCAACCGTGCCAGGGAA
    GAAGGCATCACGCCTCAGGAGGTTGCGGATAAGTACATCGCTGCCTTTCGTGAGGATGTG
    ACGGCCTTGGGCGTGAAACCTGCGACTCGCCATCCGCGTGTAGTGGAGTTTATGGCAGAC
    ATCATCCGTTTTGTGGAAGACTTGATCGAGAAAGGCTTTGCCTATGAGAGTCAAGGGGAT
    GTCTATTTCCGTGTAGAAAAATCCCACAACTATGCTAAATTGGCTAATAAAACCTTGGAA
    GATTTGGAGCTAGGTGCTTCAGGTCGTACCGATGAAGAAACGGCTCGTAAGGAAAATCCT
    GTAGACTTTGCTCTATGGAAATCTTCCAAACCAGGTGAGATTTCTTGGGATAGTCCTTGG
    GGACCTGGACGTCCGGGTTGGCATATTGAGTGTTCAGTCATGTCGACAGAGATTTTAGGT
    GATACCATTGATATCCACGGTGGTGGAGCTGACCTAGAGTTTCCACACCACACCAATGAA
    ATTGCCCAATCTGAAGCTAAAACAGGCAAGGCTTTTGCTAACTACTGGATGCACAATGGC
    TTTGTCAATATCGACAATGTCAAAATGTCTAAGTCCTTGGGTAACTTCATTACGGTACAC
    GATGCCCTTAAAACTCTTGATGGGCAAGTACTTCGTTTCTTCTTTGCGACCCAGCACTAC
    CGTAAGCCTATCAACTTCACGGAAAAGGCAGTTCGCGATGCCGAGACCAATCTCAAGTAT
    CTGAAGAATACTTACGAACAACCATTTACAGGAAACGTGGATGCCCAAGAGTTACAAAAC
    TTTAAAGATAAGTTTGTAGCAGCTATGGATGAAGATTTTAATGCTGCCAACGGTATCACA
    GTTGTCTTTGAAATGGCCAAATGGATCAACTCAGGGAACTATGATGCAAGTGTCAAGCAA
    GCTCTTGCAGATATGTTAGAAATTTTTGGAATTGTCTTTGTTGAGGAAGTTTTGGATGCA
    GAGATTGAAGACTTGATTCAAAAACGCCAAGAGGCGCGTGCCAATCGAGACTTTGAGACA
    GCAGACCAAATCCGTGACCAATTGGTTACTCAAGGAATTAAGCTCCTTGATACCAAGGAT
    GGAGTGAGGTGGACACGTGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1344

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN034156_RS08850 [old locus tag: SPN034156_15920 ]
  • symbol: CysS
  • description: cysteine--tRNA ligase
  • length: 447
  • theoretical pI: 4.9052
  • theoretical MW: 51053
  • GRAVY: -0.467785

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.16?  ExPASy
    Cysteine--tRNA ligase ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 554.9)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 280.9)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 87.7)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 33)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 31.6)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 29.5)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 25.4)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 25)
  • TheSEED: see SPN034156_15920
  • PFAM:
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 442.3)
    and 6 more
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 66.7)
    DALR (CL0258) DALR_2; DALR domain (PF09190; HMM-score: 59.2)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 38.6)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 26.1)
    tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 16.4)
    AAA_lid (CL0671) UVR; UvrB/uvrC motif (PF02151; HMM-score: 15.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9981
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0019
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018192
    • TAT(Tat/SPI): 0.000489
    • LIPO(Sec/SPII): 0.003258
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000591096 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLEDLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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