PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 06-FEB-2015

⊟Summary

• organism: Streptococcus pneumoniae ATCC 700669
• locus tag: SPN23F03050 [new locus tag: SPN23F_RS01585 ]
• pan locus tag^?: PNEUPAN001088000
• symbol: SPN23F03050
• pan gene symbol^?: yorfE
• synonym:
• product: putative DNA-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPN23F03050 [new locus tag: SPN23F_RS01585 ]
• symbol: SPN23F03050
• product: putative DNA-binding protein
• replicon: chromosome
• strand: -
• coordinates: 290225..290419
• length: 195
• essential: unknown

⊟Accession numbers

• Location: FM211187 (290225..290419) NCBI
• BioCyc: see SPN23F_RS01585
• MicrobesOnline: 5830191 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_0303 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  
  ATGAATCGTGTGAAAGAATTTCGCAAGGAACTGGGCATTTCCCAGCTCGAGCTCGCCAAG
  GATATCGGTGTCTCGAGACAGACCATCAATATGATTGAAAACGACAAGTACAATCCAACT
  CTGGAACTCTGTCTCAATCTCGCTCGCAGCCTCCAAACTGACCTCAACAGTCTCTTTTGG
  AAGGAAGATTTTTAA
  60
  120
  180
  195
  

⊟Protein

⊟General

• locus tag: SPN23F03050 [new locus tag: SPN23F_RS01585 ]
• symbol: SPN23F03050
• description: putative DNA-binding protein
• length: 64
• theoretical pI: 5.0014
• theoretical MW: 7531.6
• GRAVY: -0.4875

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 47)
  and 8 more
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 33.1)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 33.1)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 33.1)
  Unknown function General mobile mystery protein A (TIGR02612; HMM-score: 22.9)
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 21.6)
  Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 19.1)
  Signal transduction Regulatory functions DNA interactions DNA-binding protein, Tfx family (TIGR00721; HMM-score: 14.6)
  probable regulatory domain (TIGR03879; HMM-score: 12.2)
• TheSEED  :
  • Transcriptional regulator, PBSX family
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 63.4)
  and 16 more
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 47.1)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 32.3)
  HTH_25; Helix-turn-helix domain (PF13413; HMM-score: 19.3)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 18.4)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 17.4)
  Phage_CI_repr; Bacteriophage CI repressor helix-turn-helix domain (PF07022; HMM-score: 16.7)
  no clan defined Ran-binding; RanGTP-binding protein (PF05508; HMM-score: 16.6)
  HTH (CL0123) NUMOD1; NUMOD1 domain (PF07453; HMM-score: 16.1)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15.6)
  HTH_11; HTH domain (PF08279; HMM-score: 15.3)
  YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 15.1)
  CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 14.8)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 13.6)
  HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 12.8)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.8)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9591
  • Cytoplasmic Membrane Score: 0.0116
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0289
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005079
  • TAT(Tat/SPI): 0.000763
  • LIPO(Sec/SPII): 0.000889
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: CAR68163 NCBI
• UniProt:

⊟Protein sequence

• MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELCLNLARSLQTDLNSLFWKEDF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPN23F03030 < SPN23F03040 < SPN23F03050

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0303 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications