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NCBI: 06-FEB-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ATCC 700669
  • locus tag: SPN23F07460 [new locus tag: SPN23F_RS03795 ]
  • pan locus tag?: PNEUPAN001793000
  • symbol: folD
  • pan gene symbol?: folD
  • synonym:
  • product: FolD bifunctional protein [includes: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN23F07460 [new locus tag: SPN23F_RS03795 ]
  • symbol: folD
  • product: FolD bifunctional protein [includes: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase]
  • replicon: chromosome
  • strand: +
  • coordinates: 729956..730834
  • length: 879
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    TTGAAAGAAAAGAGAAGTGATATGACACAGATTATTGATGGGAAAGCTTTAGCGGCCAAA
    TTGCAGGGGCAGTTGGCTGAAAAGACTGCAAAATTAAAGGAAGAAACAGCTCTAGTGCCT
    GGTTTGGTAGTGATTTTGGTTGGGGATAATCCAGCCAGCCAAGTCTACGTTCGCAACAAG
    GAGAGGTCAGCTCTTGCGGCTGGTTCCCGTAGCGAAGTAGTGCGAGTTCCAGAGACCATT
    ACTCAAGAGGAATTGTTAGACCTGATTGCTAAATACAATCAGGATCCAGCTTGGCATGGG
    ATTTTGGTTCAGTTGCCATTACCAAAACACATTGATGAAGAGGCGGTTCTATTGGCTATT
    GACCCAGAAAAGGATGTGGATGGTTTCCATCCTCTAAATATGGGACGCCTTTGGTCTGGA
    CATCCAGTCATGATTCCATCAACACCTGCAGGAATTATGGAAATGTTTCATGAATATGGG
    ATTGAATTAGAAGGTAAAAATGCGGTTGTCATCGGTCGTTCTAATATCGTTGGAAAACCC
    ATGGCTCAGCTTCTTTTGGCCAAGAATGCAACAGTGACTTTGACCCACTCACGCACCCAT
    AATCTTGCTAAGGTGGCTGCTAAAGCAGATATTCTGGTGGTCGCAATCGGTCGTGCCAAG
    TTTGTGACTGCTGACTTTGTCAAACCAGGTGCGGTAGTCATTGACGTTGGGATGAATCGT
    GATGAAAATGGTAAGCTCTGTGGGGATGTGGATTATGAGGCAGTTGCCCCACTTGCTAGC
    TATATTACGCCAGTCCCTGGAGGTGTCGGCCCTATGACCATTACTATGCTGATGGAGCAA
    ACTTATCAAGCAGCACTTCGGACATTGGATAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN23F07460 [new locus tag: SPN23F_RS03795 ]
  • symbol: FolD
  • description: FolD bifunctional protein [includes: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase]
  • length: 292
  • theoretical pI: 6.63935
  • theoretical MW: 31594.5
  • GRAVY: -0.00924657

⊟Function[edit | edit source]

  • reaction:
    EC 1.5.1.5?  ExPASy
    Methylenetetrahydrofolate dehydrogenase (NADP+) 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH
  • TIGRFAM:
  • TheSEED  :
    • Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)
    • Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)
    Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines  Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)
    and 4 more
    Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines  Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)
  • PFAM:
    NADP_Rossmann (CL0063) THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 237.7)
    and 3 more
    AA_dh_N (CL0603) THF_DHG_CYH; Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain (PF00763; HMM-score: 137.3)
    NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 14.5)
    GT-B (CL0113) Sucrose_synth; Sucrose synthase (PF00862; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009042
    • TAT(Tat/SPI): 0.001387
    • LIPO(Sec/SPII): 0.001463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CAR68589 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQAALRTLDRK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MtaR* (repression) regulon
    MtaR*(TF)important in Methionine metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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