⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae ATCC 700669
- locus tag: SPN23F_RS01770 [old locus tags: SPN23F03440 SPN23F_03440 ]
- pan locus tag?: PNEUPAN001206000
- symbol: gpsB
- pan gene symbol?: gpsB
- synonym:
- product: cell division regulator GpsB
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPN23F_RS01770 [old locus tags: SPN23F03440 SPN23F_03440 ]
- symbol: gpsB
- product: cell division regulator GpsB
- replicon: chromosome
- strand: +
- coordinates: 334949..335290
- length: 342
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_011900 (334949..335290) NCBI
- BioCyc: SPN23F_RS01770 BioCyc
- MicrobesOnline: see SPN23F03440
- PneumoBrowse for strain D39V: SPV_0339 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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121
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241
301ATGACAAGTATTATTTTTTCAGCAAAAGATATTTTTGAACAAGAGTTTGGACGTGAAGTT
CGTGGGTATAGCAAGGTAGAAGTTGATGAGTTTTTAGACGATGTCATCAAGGACTATGAA
ACCTATGCTACCTTGGTTAAGTCACTTCGTCAGGAGATTGCGGATTTGAAGGAAGAATTA
ACTCGTAAACCGCAAGTGAGTTCGGCTCCAAGTCCTAGTCACCCAGATCCAATTGATGTG
GCTGCTTCATCTTCTATGACGAATTTTGATATTTTGAAACGCTTGAATCGTCTTGAAAAA
GAAGTATTCGGTAAACAAATTTTAGACAATACTGATTTGTAA60
120
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342
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPN23F_RS01770 [old locus tags: SPN23F03440 SPN23F_03440 ]
- symbol: GpsB
- description: cell division regulator GpsB
- length: 113
- theoretical pI: 4.44301
- theoretical MW: 12899.4
- GRAVY: -0.476991
⊟Function[edit | edit source]
- TIGRFAM: DivIVA domain (TIGR03544; HMM-score: 44.4)
- TheSEED: see SPN23F03440
- PFAM: no clan defined DivIVA; DivIVA protein (PF05103; HMM-score: 52.9)and 4 moreLMP; LMP repeated region (PF04778; HMM-score: 16.1)E-set (CL0159) ATG19; Autophagy protein Atg19, Atg8-binding (PF12744; HMM-score: 15.1)Glyco_hydro_tim (CL0058) Glyco_hydro_18; Glycosyl hydrolases family 18 (PF00704; HMM-score: 15)no clan defined DUF4634; Domain of unknown function (DUF4634) (PF15465; HMM-score: 13.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9896
- Cytoplasmic Membrane Score: 0.0091
- Cell wall & surface Score: 0.0009
- Extracellular Score: 0.0004
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001999
- TAT(Tat/SPI): 0.000778
- LIPO(Sec/SPII): 0.000405
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_000200645 NCBI
- UniProt: see SPN23F03440
⊟Protein sequence[edit | edit source]
- MTSIIFSAKDIFEQEFGREVRGYSKVEVDEFLDDVIKDYETYATLVKSLRQEIADLKEELTRKPQVSSAPSPSHPDPIDVAASSSMTNFDILKRLNRLEKEVFGKQILDNTDL
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SPN23F_RS01765 > gpsB > rnpB > SPN23F_RS01780 > mapZ > gndA > SPN23F_RS01795
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0339 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Aurore Fleurie, Sylvie Manuse, Chao Zhao, Nathalie Campo, Caroline Cluzel, Jean-Pierre Lavergne, Céline Freton, Christophe Combet, Sébastien Guiral, Boumediene Soufi, Boris Macek, Erkin Kuru, Michael S VanNieuwenhze, Yves V Brun, Anne-Marie Di Guilmi, Jean-Pierre Claverys, Anne Galinier, Christophe Grangeasse
Interplay of the serine/threonine-kinase StkP and the paralogs DivIVA and GpsB in pneumococcal cell elongation and division.
PLoS Genet: 2014, 10(4);e1004275
[PubMed:24722178] [WorldCat.org] [DOI] (I e)Britta E Rued, Jiaqi J Zheng, Andrea Mura, Ho-Ching T Tsui, Michael J Boersma, Jeffrey L Mazny, Federico Corona, Amilcar J Perez, Daniela Fadda, Linda Doubravová, Karolína Buriánková, Pavel Branny, Orietta Massidda, Malcolm E Winkler
Suppression and synthetic-lethal genetic relationships of ΔgpsB mutations indicate that GpsB mediates protein phosphorylation and penicillin-binding protein interactions in Streptococcus pneumoniae D39.
Mol Microbiol: 2017, 103(6);931-957
[PubMed:28010038] [WorldCat.org] [DOI] (I p)