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serotype 4
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serotype 23F
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ST556
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TCH8431/19A
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serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 11-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ATCC 700669
  • locus tag: SPN23F_RS03515 [old locus tags: SPN23F06900 SPN23F_06900 ]
  • pan locus tag?: PNEUPAN001707000
  • symbol: rlmN
  • pan gene symbol?: rlmN
  • synonym:
  • product: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN23F_RS03515 [old locus tags: SPN23F06900 SPN23F_06900 ]
  • symbol: rlmN
  • product: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
  • replicon: chromosome
  • strand: +
  • coordinates: 677356..678258
  • length: 903
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACCCTCAATTCATAGTTTGGTACATCAAACCATGCAGGAATGGGTATTGGAGCAG
    GGAGAAAAGAAATTCCGTGCAGATCAAATCTGGGAATGGCTCTACCGTAAACGTGTGCAG
    TCATTTGAAGAAATGACCAACCTTTCCAAGGATTTGATTGCTAAGCTCAATGACCAGTTT
    GTGGTCAATCCCTTGAAACAACGTATTGTGCAAGAGTCTGCTGATGGTACAGTCAAATAT
    CTCTTTGAATTGCCTGACGGTATGTTAATTGAGACTGTACTCATGCGTCAACACTATGGT
    TTATCTGTCTGTGTGACCACTCAGGTCGGCTGTAATATCGGTTGTACCTTCTGTGCCTCT
    GGTTTGATTAAGAAGCAGCGTGACCTCAATAACGGTGAAATCGTAGCGCAAATCATGCTG
    GTTCAGAAATACTTTGCTGATGAAGGGGTTCAGGTCAATCTTGCCGTGTCTCTTCACGCA
    CCCAACAATGAACTTCGTTCAAGTACCATGAAGATTAACCGTGCCTTTCCGATTGAAAAA
    CTCTTTGCTGCTATTGAATACTACATTGAGACAACAAACCGTCGTGTAACCTTTGAATAT
    ATCATGCTTAATGAAGTCAACGACGGTGTAGAACAAGCCTTGGAATTGGCTGAATTGCTC
    AAGAACATCAAGAAATTGTCTTATGTAAACTTGATTCCTTATAACCCAGTTAGTGAGCAT
    GACCAATATAGCCGTAGTCCCAAAGAGCGCGTGTTGGCTTTCTATGATACGCTTAAGAAA
    AAAGGGGTCAACTGTGTGGTTCGTCAAGAGCATGGTACAGATATTGACGCAGCTTGTGGA
    CAATTGCGCTCTAACACAATGAAACGTGACCGCCAGAAGGCAGTCGCAGCGGTAAATCCT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN23F_RS03515 [old locus tags: SPN23F06900 SPN23F_06900 ]
  • symbol: RlmN
  • description: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
  • length: 300
  • theoretical pI: 8.11215
  • theoretical MW: 34422.4
  • GRAVY: -0.333333

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.192?  ExPASy
    23S rRNA (adenine2503-C2)-methyltransferase 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin 2 S-adenosyl-L-methionine + adenine37 in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(2))-methyltransferase (TIGR00048; EC 2.1.1.192; HMM-score: 344.4)
    and 7 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(8))-methyltransferase Cfr (TIGR04432; EC 2.1.1.224; HMM-score: 202.6)
    Genetic information processing Protein fate Protein modification and repair pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 21.6)
    Metabolism Energy metabolism Anaerobic pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 21.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 16.2)
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 14.2)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage major tail tube protein (TIGR01611; HMM-score: 13.2)
    AmmeMemoRadiSam system radical SAM enzyme (TIGR04337; HMM-score: 12.1)
  • TheSEED: see SPN23F06900
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 29.7)
    and 3 more
    no clan defined P63C; P63C domain (PF10546; HMM-score: 14.5)
    TIM_barrel (CL0036) Radical_SAM_2; Radical SAM-like domain (PF19238; HMM-score: 13.7)
    HTH (CL0123) TFIIE_beta; TFIIE beta subunit core domain (PF02186; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8122
    • Cytoplasmic Membrane Score: 0.1442
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0431
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0034
    • TAT(Tat/SPI): 0.000181
    • LIPO(Sec/SPII): 0.000393
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000804658 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHAPNNELRSSTMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVAAVNP

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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