PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 03-APR-2017

⊟Summary

• organism: Streptococcus pneumoniae Hu17
• locus tag: SPNHU17_01740 [new locus tag: SPNHU17_RS08125 ]
• pan locus tag^?: PNEUPAN003115000
• symbol: SPNHU17_01740
• pan gene symbol^?: dnaI
• synonym:
• product: primosomal protein DnaI

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNHU17_01740 [new locus tag: SPNHU17_RS08125 ]
• symbol: SPNHU17_01740
• product: primosomal protein DnaI
• replicon: chromosome
• strand: -
• coordinates: 1620278..1621174
• length: 897
• essential: unknown other strains

⊟Accession numbers

• Location: CP020549 (1620278..1621174) NCBI
• BioCyc: see SPNHU17_RS08125
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1521 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGGAAAGTGTCGGAGACGTACTCAAACGTCAACCCAGCCGTTTTTATTATCAAGATTTG
  GTCCAGAAAATCATGAAGGACCCTGATGTTGCGGCTTTTATCCAGCAAGAATCCTTGACT
  CCAGAGGAATTAAATCGCAGTATCTCCAAGTTTAATCAGTACATCACCGAGCGTGACAAG
  TTTCTCCGTGGGGATACGGATTATATTGCCAAAGGCTACAAGCCGATTTTGGTTAAGAAT
  CATGGCTATGCAGATGTTTCATATGAAGAAACTCCTGAGCTAATCGCGGCTGAAAAAGAA
  GCGGCTATTAAGAACCGTCTCAAGTTAATCAATCTACCAGCCAGTCTCAAGAAAGCTAGT
  TTGGCTCAAGTTGACTTGGATGATTTGGGGCGCTTACCAGTTTTTGAAAAGCTACTAGCC
  TTCGTGGAGCAATATCCAGCTATTCGAAAAGGTCTTTACTTGTATGGAGACTTTGGTGTG
  GGTAAAAGTTTCATGGTGGCTGCCCTAGCTCATGATTTATCAGAAAAACGTGGTGTATCA
  TCCACTCTCCTCCACTATCCTAGCTTTGTCATTGATGTCAAAAATGCTATTGGTGATGGC
  AATGTTAAGACCTTAGTGGATGAGATTAAGCTTTCTGAAGTCCTGATTTTAGATGATATT
  GGTGCCGAGCAATCCACCGCTTGGGTGCGGGATGAAATCCTACAAGTCATTCTCCAATAT
  CGGATGCAGGAAAATTTACCGACCTTTTTCACGTCCAACTTCAACTTTGAAGAATTGGAG
  CTGCATTTCGCTAAAGGGAAGCATGGAAATGACGAAACCTGGGAAGCTAGACGGGTCATG
  GAACGCCTCCGTTATTTGTCTGAGGAGACTCGTTTAGAAGGAGTGAACCGTCGATGA
  60
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  840
  897
  

⊟Protein

⊟General

• locus tag: SPNHU17_01740 [new locus tag: SPNHU17_RS08125 ]
• symbol: SPNHU17_01740
• description: primosomal protein DnaI
• length: 298
• theoretical pI: 5.31556
• theoretical MW: 34174.7
• GRAVY: -0.36745

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 27)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 17.8)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  no clan defined DnaI_N; Primosomal protein DnaI N-terminus (PF07319; HMM-score: 75.8)
  and 12 more
  P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 35.8)
  Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 25.1)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 22.7)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 18.7)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 18)
  AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 16.3)
  KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 16.3)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 15.2)
  DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 14.1)
  AAA_22; AAA domain (PF13401; HMM-score: 13.2)
  Globin (CL0090) Bac_globin; Bacterial-like globin (PF01152; HMM-score: 13)
  no clan defined STI1; STI1 domain (PF17830; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.78
  • Cytoplasmic Membrane Score: 8.16
  • Cellwall Score: 0.06
  • Extracellular Score: 0.01
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002657
  • TAT(Tat/SPI): 0.000209
  • LIPO(Sec/SPII): 0.000396
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ARD35294 NCBI
• UniProt:

⊟Protein sequence

• MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEELNRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAAIKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGVGKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILDDIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEELELHFAKGKHGNDETWEARRVMERLRYLSEETRLEGVNRR

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1521 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications