From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-OCT-2019

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NU83127
  • locus tag: SPNU_0138 [new locus tag: EL277_RS12125 ]
  • pan locus tag?: PNEUPAN000811000
  • symbol: bshA
  • pan gene symbol?: bshA
  • synonym:
  • product: N-acetyl-alpha-D-glucosaminyl L-malate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNU_0138 [new locus tag: EL277_RS12125 ]
  • symbol: bshA
  • product: N-acetyl-alpha-D-glucosaminyl L-malate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 143184..143708
  • length: 525
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AP018936 (143184..143708) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    TTGGATCCAGAAAAAAGGCCGGATAGATTCCTTGAATTTGCAGAAAAATTATTTTTAGTC
    AGAGAAGATGTGATTTTTATTATGGCTGGAAATGGTAGTATGTGGGCTGCTTTGAAAGAA
    AAAATTTGCCATTTGAAATGTAGAGATAATTTCAGGTTACTTGGTGAAATCTATCCAGCT
    ACTATAGTTTACCAAATATCGGATTTATTGTATATTCCTTCTGATACAGAAGGAATACCT
    ATGTGTGTTTTAGAGTCAATGTCACAAGGAACTCCGGTTTTAGCTAGTAATGTTGGCGGG
    TTAAGTGAAATTATTGAACATAGGGTTGATGGATTTTTATTTGAGAAGGAAGATGTTGAG
    GGAGTGTGTGCTTGTGCTAATTTTTTACTCAATGATTCTGAGTATTTGAAATATATAGGT
    GAGAATAGTAAATCAAAAATAAGAAAACATTTTTCTGTGCAAAAAATGTTTGTAGAAACC
    ATGAGAGTATATGATGAATTATTAGAGAAGAGTAGTCATGGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    525

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNU_0138 [new locus tag: EL277_RS12125 ]
  • symbol: BshA
  • description: N-acetyl-alpha-D-glucosaminyl L-malate synthase
  • length: 174
  • theoretical pI: 4.81112
  • theoretical MW: 19868.8
  • GRAVY: -0.114943

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 91.1)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 90.5)
    and 12 more
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 53.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 47.2)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 41)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 33.8)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 31.8)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 29)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 28.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 28.3)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 26.7)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 20.7)
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 14.5)
    colanic acid biosynthesis glycosyltransferase WcaC (TIGR04015; EC 2.4.-.-; HMM-score: 11.4)
  • TheSEED: data available for Hungary19A-6, TIGR4
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 90.1)
    and 2 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 67.9)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 31)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7098
    • Cytoplasmic Membrane Score: 0.0624
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.2268
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005161
    • TAT(Tat/SPI): 0.002572
    • LIPO(Sec/SPII): 0.001796
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: BBG34029 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPNU_0138&oldid=115962"