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serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae P1031
  • locus tag: SPP_0595 [new locus tag: SPP_RS02940 ]
  • pan locus tag?: PNEUPAN001471000
  • symbol: pheS
  • pan gene symbol?: pheS
  • synonym:
  • product: phenylalanyl-tRNA synthetase, alpha subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPP_0595 [new locus tag: SPP_RS02940 ]
  • symbol: pheS
  • product: phenylalanyl-tRNA synthetase, alpha subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 536104..537192
  • length: 1089
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTGGTTTTCTTTTTATCTACGAGAGACTGATGGAGGAAATATGTCAACTATTGAAGAA
    CAATTAAAAGCGCTTCGCGAAGAAACGCTGACTAGCTTGAAGCAGATTACTGCTGGAAAT
    GAAAAAGAGATGCAAGATTTGCGTGTCTCTGTCCTTGGTAAAAAGGGTTCGCTCACTGAA
    ATCCTCAAAGGGATGAAAGATGTTTCTGCTGAGATGCGTCCAATCATCGGGAAACACGTC
    AATGAAGCTCGTGATGTCTTGACAGCTGCTTTTGAAGAAACAGCTAAGCTCTTGGAAGAA
    AAGAAAGTCGCGGCTCAACTGGCTAGCGAGAGTATCGATGTGACGCTTCCAGGTCGTCCA
    GTTGCGACTGGTCACCGTCACGTTTTGACACAAACCAGTGAAGAAATCGAAGATATCTTC
    ATCGGTATGGGTTATCAAGTCGTGGATGGTTTTGAAGTGGAGCAAGACTACTATAACTTT
    GAACGTATGAACCTTCCAAAAGACCACCCAGCTCGTGATATGCAGGATACTTTCTATATC
    ACTGAAGAAATCTTGCTCCGTACCCACACGTCTCCAGTTCAGGCGCGTGCTATGGATGCC
    CATGATTTTTCTAAAGGTCCTTTGAAGATGATCTCGCCAGGGCGTGTCTTCCGTCGCGAT
    ACGGACGATGCGACCCACAGTCACCAATTCCACCAAATCGAAGGCTTGGTAGTTGGGAAA
    AATATCTCTATGGCTGATCTTCAAGGAACGCTTCAGTTGATTGTCCAAAAAATGTTTGGT
    GAAGAGCGTCAAATTCGTTTGCGTCCATCTTACTTCCCATTCACAGAGCCATCTGTTGAG
    GTGGATGTTTCTTGCTTCAAGTGTGGTGGAGAAGGCTGTAACGTATGTAAGAAAACAGGT
    TGGATCGAAATTATGGGAGCCGGTATGGTTCACCCACGTGTCCTTGAAATGAGTGGTATC
    GATGCGACTGTATACTCTGGTTTTGCCTTTGGTCTTGGACAAGAGCGTGTAGCTATGCTC
    CGTTATGGAATCAACGATATCCGTGGATTCTACCAAGGAGATGTCCGCTTCTCAGAACAG
    TTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1089

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPP_0595 [new locus tag: SPP_RS02940 ]
  • symbol: PheS
  • description: phenylalanyl-tRNA synthetase, alpha subunit
  • length: 362
  • theoretical pI: 5.32558
  • theoretical MW: 40836.3
  • GRAVY: -0.353039

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.20?  ExPASy
    Phenylalanine--tRNA ligase ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 357.6)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase (TIGR00469; EC 6.1.1.20; HMM-score: 114.5)
    Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 51.5)
    Metabolism Amino acid biosynthesis Serine family O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 51.5)
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 23.7)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 18)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00471; EC 6.1.1.20; HMM-score: 17.5)
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 16.4)
  • TheSEED  :
    • Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Phe  Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 360.8)
    and 5 more
    tRNA_bind_arm (CL0298) Phe_tRNA-synt_N; Aminoacyl tRNA synthetase class II, N-terminal domain (PF02912; HMM-score: 70.7)
    tRNA_synt_II (CL0040) tRNA_synthFbeta; Phenylalanyl tRNA synthetase beta chain CLM domain (PF17759; HMM-score: 16.6)
    no clan defined KfrA_N; Plasmid replication region DNA-binding N-term (PF11740; HMM-score: 14.6)
    tRNA_synt_II (CL0040) tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 12.4)
    YbjQ-like (CL0522) YbjQ_1; Putative heavy-metal-binding (PF01906; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9977
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0022
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003755
    • TAT(Tat/SPI): 0.001015
    • LIPO(Sec/SPII): 0.000522
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACO21874 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MWFSFYLRETDGGNMSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDMQDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIRGFYQGDVRFSEQFK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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