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serotype 4
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serotype 6B
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serotype 19A
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serotype 14
JJA
serotype 14
MDRSPN001
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NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
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serotype 1
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serotype 2
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serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
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SPN994038
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SPN994039
serotype 3
SPNA45
serotype 3
ST556
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TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae P1031
  • locus tag: SPP_0935 [new locus tag: SPP_RS04610 ]
  • pan locus tag?: PNEUPAN001934000
  • symbol: lspA
  • pan gene symbol?: lspA
  • synonym:
  • product: signal peptidase II

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPP_0935 [new locus tag: SPP_RS04610 ]
  • symbol: lspA
  • product: signal peptidase II
  • replicon: chromosome
  • strand: +
  • coordinates: 859036..859497
  • length: 462
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAAAAAGAGCAATAGTGGCAGTCATTGTACTGCTTTTAATTGGGCTGGATCAGTTG
    GTCAAATCCTATATCGTCCAGCAGATTCCACTGGGTGAAGTGCGCTCCTGGATTCCCAAT
    TTCGTTAGCTTGACCTACCTGCAAAATCGAGGTGCAGCCTTTTCTATCTTACAAGATCAG
    CAGCTGTTATTCGCTGTCATTACTCTGGTTGTCGTGATAGGTGCCATTTGGTATTTACAT
    AAACACATGGAGGACTCATTCTGGATGGTCTTGGGTTTGACTTTGATAATCGCGGGTGGT
    CTTGGAAACTTTATTGACAGGGCCAGTCAGGGCTTTGTTGTGGATATGTTCCACCTTGAC
    TTTATCAACTTTGCAATTTTCAATGTGGCAGATAGCTATCTGACGGTTGGAGTGATTATT
    TTATTGATTGCAATGCTAAAAGAGGAAATAAATGGAAATTAA
    60
    120
    180
    240
    300
    360
    420
    462

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPP_0935 [new locus tag: SPP_RS04610 ]
  • symbol: LspA
  • description: signal peptidase II
  • length: 153
  • theoretical pI: 6.23647
  • theoretical MW: 17122.3
  • GRAVY: 0.887582

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.23.36?  ExPASy
    Signal peptidase II Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains
  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase II (TIGR00077; EC 3.4.23.36; HMM-score: 122.6)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 5.1)
  • TheSEED  :
    • Lipoprotein signal peptidase (EC 3.4.23.36)
    Protein Metabolism Protein processing and modification Lipoprotein Biosynthesis  Lipoprotein signal peptidase (EC 3.4.23.36)
    and 1 more
    Protein Metabolism Protein processing and modification Signal peptidase  Lipoprotein signal peptidase (EC 3.4.23.36)
  • PFAM:
    no clan defined Peptidase_A8; Signal peptidase (SPase) II (PF01252; HMM-score: 127.7)
    and 4 more
    DUF6594; Family of unknown function (DUF6594) (PF20237; HMM-score: 11.8)
    MFS (CL0015) OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 10.9)
    no clan defined PrgI; PrgI family protein (PF12666; HMM-score: 9.3)
    TMEM_230_134; Transmembrane proteins 230/134 (PF05915; HMM-score: 7.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.501891
    • TAT(Tat/SPI): 0.000482
    • LIPO(Sec/SPII): 0.044322
    • Cleavage Site: CS pos: 23-24. VKS-YI. Pr: 0.4361
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACO20189 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSLTYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLGNFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Suneeta Khandavilli, Karen A Homer, Jose Yuste, Shilpa Basavanna, Timothy Mitchell, Jeremy S Brown
Maturation of Streptococcus pneumoniae lipoproteins by a type II signal peptidase is required for ABC transporter function and full virulence.
Mol Microbiol: 2008, 67(3);541-57
[PubMed:18086214] [WorldCat.org] [DOI] (P p)

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