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JJA
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serotype 1
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serotype 3
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SPN994038
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TCH8431/19A
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serotype 19F
Xen35
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serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae P1031
  • locus tag: SPP_1401 [new locus tag: SPP_RS06905 ]
  • pan locus tag?: PNEUPAN002707000
  • symbol: SPP_1401
  • pan gene symbol?: amy
  • synonym:
  • product: alpha-amylase (1,4-alpha-D-glucanglucanohydrolase)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPP_1401 [new locus tag: SPP_RS06905 ]
  • symbol: SPP_1401
  • product: alpha-amylase (1,4-alpha-D-glucanglucanohydrolase)
  • replicon: chromosome
  • strand: +
  • coordinates: 1308988..1310442
  • length: 1455
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAAAATCAAACGCTCATGCAATACTTTGAATGGTATCTGCCCCACGACGGTCAGCAC
    TGGACGCGTCTGGCTGAAGATGCTCCACACCTAGCTCATCTGGGGATTAGCCACGTCTGG
    ATGCCACCAGCTTTTAAGGCAACCAATGAAAAAGATGTCGGCTATGGTGTCTATGACTTA
    TTTGACCTAGGAGAGTTTAACCAAAAAGGGACTGTCCGCACTAAGTATGGTTTCAAAGAA
    GACTATCTTCAAGCCATTCAAGCCCTAAAAGCACAGGGAATTCAACCTATGGCCGATGTG
    GTTCTCAATCACAAGGCTGCAGCCGATCACATGGAAGCCTTTCAGGTTATCGAAGTGGAT
    CCTGTAGACCGTACGGTTGAACTTGGAGAACCCTTCACCATCAATGGCTGGACTAGCTTT
    ACCTTCTATGGTCGCCAAGATACCTACAATGACTTCCACTGGCATTGGTACCACTTCACC
    GGTACAGACTACGATGCAAAACGCCGTAAGTCTGGGATTTATCTCATCCAAGGGGACAAC
    AAGGGCTGGGCTAACGAGGAATTGGTCGATAACGAAAACGGAAACTACGACTACCTCATG
    TATGCCGACCTAGACTTTAAACATCCTGAAGTCATCCAAAACATCTATGACTGGGCTGAT
    TGGTTCATGGAAACGACTGGTGTAGCTGGTTTCCGTTTGGATGCCGTTAAGCATATTGAC
    TCTTTCTTTATGCGCAACTTCATCCGCGATATGAAGGAAAAATACGGTGACGATTTCTAT
    GTTTTTGGTGAATTTTGGAACCCAGACAAGGAAGCCAATCTGGACTATCTCGAAAAAACG
    GAAGAACACTTTGACCTTGTCGATGTTCGTCTCCACCAGAATCTCTTTGAAGCCAGTCAA
    GCTGGCGCAAACTATGACCTTCGTGGCATTTTCACAGATAGCCTGGTTGAACTCAAGCCT
    GACAAGTCTGTGACTTTTGTCGACAACCACGATACCCAACGAGGACAAGCCCTTGAGTCT
    ACCGTTGAAGAATGGTTCAAGCCAGCAGCCTATGCCCTCATTTTGTTACGCCAAGACGGC
    CTTCCATGTGTCTTTTACGGAGACTACTATGGGATTTCAGGCCAGTATGCTCAACAAGAT
    TTCAAAGAAATCCTTGACCGCCTCCTAGCCATCCGAAAAGATTTGGCCTATGGAGAACAA
    AATGACTACTTTGACCATGCTAACTGTATCGGTTGGGTACGTTCAGGTGCTGAAAATCAA
    TCCCCAATCGCAGTCTTGATTTCAAATGACCAAGAAAACAGCAAGTCAATGTTTGTCGGT
    CAAGAATGGACTAATCAAACCTTTGTAGATTTACTTGGTAACCACCAAGGTCAAGTTACA
    ATTGATGAGGAAGGTTATGGACAATTCCCTGTCTCAGCTAGATCCGTAAGTGTCTGGGCA
    GTCAATACCATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1455

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPP_1401 [new locus tag: SPP_RS06905 ]
  • symbol: SPP_1401
  • description: alpha-amylase (1,4-alpha-D-glucanglucanohydrolase)
  • length: 484
  • theoretical pI: 4.41395
  • theoretical MW: 56083.7
  • GRAVY: -0.531405

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.1?  ExPASy
    Alpha-amylase Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 45.1)
    and 3 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 28.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 26.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 19.7)
  • TheSEED  :
    • Cytoplasmic alpha-amylase (EC 3.2.1.1)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Cytoplasmic alpha-amylase (EC 3.2.1.1)
    and 1 more
    Carbohydrates Polysaccharides Glycogen metabolism  Cytoplasmic alpha-amylase (EC 3.2.1.1)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 117.3)
    and 2 more
    GHD (CL0369) Alpha-amy_C_pro; Alpha-amylase C-terminal (PF09154; HMM-score: 51.2)
    Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 39.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011468
    • TAT(Tat/SPI): 0.000372
    • LIPO(Sec/SPII): 0.000891
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACO20564 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MQNQTLMQYFEWYLPHDGQHWTRLAEDAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVLNHKAAADHMEAFQVIEVDPVDRTVELGEPFTINGWTSFTFYGRQDTYNDFHWHWYHFTGTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKSVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIRKDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MalR* (repression) regulon
    MalR*(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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