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serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Taiwan19F-14
  • locus tag: SPT_1649 [new locus tag: SPT_RS08200 ]
  • pan locus tag?: PNEUPAN003115000
  • symbol: SPT_1649
  • pan gene symbol?: dnaI
  • synonym:
  • product: primosomal protein DnaI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPT_1649 [new locus tag: SPT_RS08200 ]
  • symbol: SPT_1649
  • product: primosomal protein DnaI
  • replicon: chromosome
  • strand: -
  • coordinates: 1574045..1574941
  • length: 897
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAAGTGTCGGAGACGTACTCAAACGTCAACCCAGCCGTTTTTATTATCAAGATTTG
    GTCCAGAAAATCATGAAGGACCCTGATGTTGCGGCTTTTATCCAGCAAGAATCCTTGACT
    CCAGAGGAATTAAATCGCAGTATCTCCAAGTTTAATCAGTACATCACCGAGCGTGACAAG
    TTTCTCCGTGGGGATACGGATTATATTGCCAAAGGCTACAAGCCGATTTTGGTTAAGAAT
    CATGGCTATGCAGATGTTTCATATGAAGAAACTCCTGAGCTAATCGCGGCTGAAAAAGAA
    GCGGCTATTAAGAACCGTCTCAAGTTAATCAATCTACCAGCCAGTCTCAAGAAAGCTAGT
    TTGGCTCAAGTTGACTTGGATGATTTGGGGCGCTTACCAGTTTTTGAAAAGCTACTAGCC
    TTCGTGGAGCAATATCCAGCTATTCGAAAAGGTCTTTACTTATATGGAGACTTTGGTGTG
    GGTAAAAGTTTCATGGTGGCGGCCTTAGCTCATGATTTATCAGAAAAACGTGGTGTTTCA
    TCAACTCTCCTCCACTATCCTAGCTTTGTCATTGATGTCAAAAATGCTATCAGTGATGGC
    AATGTTAAGACCTTGGTGGATGAGATTAAGCTGTCTGAGGTCCTGATTTTAGATGATATT
    GGCGCCGAGCAATCAACCGTTTGGGTGCGTGACGAAATCCTGCAGGTCATTCTCCAATAT
    CGGATGCAGGAAAATTTACCGACCTTTTTCACATCCAACTTCAACTTTGAAGATTTGGAG
    AAGCATTTTGCGAAAGTGAAACATGGAAATGACGAAACCTGGGAAGCCAGACGGGTCATG
    GAACGCATCCGTTATTTGGCTGAGGAGACTCGTTTAGAAGGAGTAAACCGTCGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    897

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPT_1649 [new locus tag: SPT_RS08200 ]
  • symbol: SPT_1649
  • description: primosomal protein DnaI
  • length: 298
  • theoretical pI: 5.51546
  • theoretical MW: 34259.9
  • GRAVY: -0.360067

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 28.3)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 16.8)
  • TheSEED  :
    • Helicase loader DnaI
    DNA Metabolism DNA replication DNA-replication  Helicase loader DnaI
  • PFAM:
    no clan defined DnaI_N; Primosomal protein DnaI N-terminus (PF07319; HMM-score: 76.1)
    and 13 more
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 35.2)
    Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 25.8)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 23.4)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 17.9)
    KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 17.2)
    AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 16.6)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 15.3)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 14.5)
    AAA_22; AAA domain (PF13401; HMM-score: 13.3)
    Globin (CL0090) Bac_globin; Bacterial-like globin (PF01152; HMM-score: 13.1)
    P-loop_NTPase (CL0023) AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 12.7)
    no clan defined STI1; STI1 domain (PF17830; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002657
    • TAT(Tat/SPI): 0.000209
    • LIPO(Sec/SPII): 0.000396
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACO23701 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEELNRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAAIKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGVGKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILDDIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEARRVMERIRYLAEETRLEGVNRR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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