From PneumoWiki
Jump to navigation Jump to search
TIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0307
  • pan locus tag?:
  • symbol: SP_0307
  • pan gene symbol?: —
  • synonym:
  • product: PTS system, IIA component

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0307
  • symbol: SP_0307
  • product: PTS system, IIA component
  • replicon: chromosome
  • strand: +
  • coordinates: 280609..281106
  • length: 498
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AE005672 (280609..281106) NCBI
  • BioCyc:
  • MicrobesOnline: 115843 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAAGTTTCTAGTTATGTACAATCATCAAAGATTAACAAATTTGAAAACAACGGCTTT
    AGACAGTATTTTAGTAAAGAAAACTTTTTAATATGTACTGAATCAGACAAGGTAAATTTA
    CTTGAAAAGATGGTTGAGAGTTTATCTGTTGGTGAATCAAATGAGTTTGAACAATCTTTA
    CTTTATGGAATTAAACAGCGCGAAGAATTAAGTTCAGTTGTTTTTTCTGAAAAAATTGCA
    GTTCCGCATCCTATTCAGCCCTTTGGTACAGAGGGAAAGGTTTCGGTTGCAATTTGTAAA
    GATTCGTTACTCTGGGATAATCAAAGTTCACATGTCCAGCTAGTATTTCTTCTATCTCCA
    TCAATATATGGGAACGAAGGTTTGGCAACTGTGACTAAAAAGATAGTCTCTCTGACAGAG
    AATGATGAATTGCAAAATCAGCTAATATCTTGCAATAACTTTGAAGATTTTATTAACATA
    TTTGAGAAGATAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    498

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0307
  • symbol: SP_0307
  • description: PTS system, IIA component
  • length: 165
  • theoretical pI: 4.59793
  • theoretical MW: 18727.1
  • GRAVY: -0.230909

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 36.1)
    Signal transduction Signal transduction PTS PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 36.1)
    and 1 more
    Signal transduction Signal transduction PTS PTS IIA-like nitrogen-regulatory protein PtsN (TIGR01419; HMM-score: 18.6)
  • TheSEED  :
    • Transcriptional antiterminator of lichenan operon, BglG family
    Carbohydrates Di- and oligosaccharides Beta-Glucoside Metabolism  Transcriptional antiterminator of lichenan operon, BglG family
  • PFAM:
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 53.6)
    and 1 more
    no clan defined Moulting_cycle; Moulting cycle (PF04870; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors: CelB, cellobiose-specific PTS component EIIB; HPr, phosphocarrier protein
  • genes regulated by CelR, TF important in Cellobiose utilizationRegPrecise
    repression
    bglA-1
    celB* > celR* > SP_0307 > celC* > SP_0309 > celD*

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001972
    • TAT(Tat/SPI): 0.000188
    • LIPO(Sec/SPII): 0.000305
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQVSSYVQSSKINKFENNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CelR* (repression) regulon, CelR (repression) regulon, CcpA regulon
    CelR*(TF)important in Cellobiose utilization; RegPrecise 
    CelR(TF)important in Cellobiose utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP_0307&oldid=2527"