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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0359 [new locus tag: SP_RS01755 ]
  • pan locus tag?: PNEUPAN001179000
  • symbol: cps4K
  • pan gene symbol?: cps4K
  • synonym:
  • product: capsular polysaccharide biosynthesis protein Cps4K

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0359 [new locus tag: SP_RS01755 ]
  • symbol: cps4K
  • product: capsular polysaccharide biosynthesis protein Cps4K
  • replicon: chromosome
  • strand: +
  • coordinates: 334030..335259
  • length: 1230
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AE005672 (334030..335259) NCBI
  • BioCyc:
  • MicrobesOnline: 115891 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATTAAAAATATTTTAATCACAGGAGCGAAAGGATTTGTAGGAAAAAATCTTATCTGT
    ACTCTGGAAGCTTTGAAAGATGGACGAGATAGGACTCGTCCTAATTTAGAGATTGGAGAG
    ATTTTTCAGTATGATCGTGATACAGATCCGATTTTATTAGATGAATATTGTAAGAAGGCC
    GATTTCGTATTCCATTTAGCTGGTGTCAATCGTCCACAGAATCCTGATGAATTCATGGAG
    GGAAATTACGGTTTTTCAAGTAGATTATTGGAGATTTTAGAAAAGTATGAAAACACTTGT
    CCTGTTCTACTCTCAAGTTCTACTCAAGCTAGTTTAGAAGGCCGATTTTCAAACTCTATA
    TATGGACAATCTAAGCTAGCAGGGGAAGAACTCTTCTTTGAATATGGAAAGAAAACGGGA
    GCACCTGTCTTAGTTTACCGTTTCCCGAATCTTTATGGGAAGTGGTGCCGTCCTAACTAC
    AATTCTGCTGTAGCAACTTTCTGTTATAATCTAGCTCACGATTTACCTATTCAAGTAAAT
    GATCCAAGTGTAGAATTGGAGTTGCTGTATATTGATGATTTGATACAAGAGTGTCTAACT
    GCATTGGAAGGAAATCCTCATCGTTGTAATCTAGATGGATTACAAATCTTACCTAGCCCA
    TCAGGAAACTACTGCTACGTACCAACGACTCATCGTGCAACCTTAGGAGAGATTGTCTCT
    CTATTAGAAACATTTAAAAAACAGCCTGATAGTTTAGTTATGCCTGAAATTCCTCAGGGA
    TCGTTTAAAAAGAAATTGTATTCTACCTACCTATCTTATTTACCAGTAGATAAGTTTAAG
    TTTCCTCTAAAAATGAATATAGATGAACGAGGTAGTTTTACGGAATTATTAAAAACAGAA
    AATACGGGTCAATTTTCTGTTAATATTTCTAAACCTGGCATTACCAAAGGGCAACATTGG
    CATCATTCTAAGTGGGAATTTTTCATGGTTGTTTCTGGTCGTGCTTTAATACAAGAGCGT
    AGGATAGGGCTGGATGAAAACGGTCAAGAGTATCCTATTCTGAACTTTGAAGTGTCGGGC
    GATAAGATTGAAGCAATACATATGATACCGGGCTATGCACATAATATTATTAATCTTTCT
    GATACAGAAAATCTAATTACTGTCATGTGGGCTAATGAGTCATTTGATCCTAGACATCCA
    GATACTTTTTTTGAACAAGTGGAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1230

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0359 [new locus tag: SP_RS01755 ]
  • symbol: Cps4K
  • description: capsular polysaccharide biosynthesis protein Cps4K
  • length: 409
  • theoretical pI: 5.19684
  • theoretical MW: 46597.7
  • GRAVY: -0.352323

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 25.2)
    cupin fold metalloprotein, WbuC family (TIGR04366; HMM-score: 20.6)
    and 6 more
    thioester reductase domain (TIGR01746; HMM-score: 17.6)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 15.9)
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 14.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 13.5)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 11.4)
    NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 11.3)
  • TheSEED  :
    • Capsular polysaccharide synthesis enzyme Cap5F
  • PFAM:
    NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 62.3)
    and 5 more
    NAD_binding_4; Male sterility protein (PF07993; HMM-score: 27.9)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 18.5)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 17.2)
    Cupin (CL0029) Cupin_2; Cupin domain (PF07883; HMM-score: 16.9)
    NADP_Rossmann (CL0063) Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8974
    • Cytoplasmic Membrane Score: 0.0146
    • Cell wall & surface Score: 0.001
    • Extracellular Score: 0.087
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00935
    • TAT(Tat/SPI): 0.00025
    • LIPO(Sec/SPII): 0.004399
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKNILITGAKGFVGKNLICTLEALKDGRDRTRPNLEIGEIFQYDRDTDPILLDEYCKKADFVFHLAGVNRPQNPDEFMEGNYGFSSRLLEILEKYENTCPVLLSSSTQASLEGRFSNSIYGQSKLAGEELFFEYGKKTGAPVLVYRFPNLYGKWCRPNYNSAVATFCYNLAHDLPIQVNDPSVELELLYIDDLIQECLTALEGNPHRCNLDGLQILPSPSGNYCYVPTTHRATLGEIVSLLETFKKQPDSLVMPEIPQGSFKKKLYSTYLSYLPVDKFKFPLKMNIDERGSFTELLKTENTGQFSVNISKPGITKGQHWHHSKWEFFMVVSGRALIQERRIGLDENGQEYPILNFEVSGDKIEAIHMIPGYAHNIINLSDTENLITVMWANESFDPRHPDTFFEQVEK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site: 333738 [1]

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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