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PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 31-JAN-2014
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae TIGR4
- locus tag: SP_0825 [new locus tag: SP_RS04035 ]
- pan locus tag?: PNEUPAN001793000
- symbol: folD
- pan gene symbol?: folD
- synonym:
- product: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SP_0825 [new locus tag: SP_RS04035 ]
- symbol: folD
- product: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
- replicon: chromosome
- strand: +
- coordinates: 774761..775618
- length: 858
- essential: yes [1]
⊟Accession numbers[edit | edit source]
- Location: AE005672 (774761..775618) NCBI
- BioCyc: see SP_RS04035
- MicrobesOnline: 116316 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_0721 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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841ATGACACAGATTATTGATGGGAAAGCTTTAGCGGCCAAATTGCAGGGGCAGTTGGCTGAA
AAGACTGCAAAATTAAAGGAAGAAACAGGTCTAGTGCCTGGTTTGGTAGTGATTTTGGTT
GGGGACAATCCAGCCAGCCAAGTCTACGTTCGCAACAAGGAGAGGTCAGCCCTTGCGGCT
GGTTTCCGTAGCGAAGTAGTACGGGTTCCAGAGACCATTACTCAAGAGGAATTGTTAGAC
CTGATTGCTAAATACAATCAGGATCCAGCTTGGCATGGGATTTTGGTTCAGTTGCCATTA
CCAAAACACATTGATGAAGAGGCGGTTCTATTGGCTATTGACCCAGAAAAGGATGTGGAT
GGTTTCCATCCTCTAAACATGGGGCGTCTTTGGTCTGGTCATCCAGTCATGATTCCTTCG
ACACCGGCAGGAATTATGGAAATGTTCCATGAATATGGGATTGACTTGGAAGGTAAAAAT
GCAGTCGTCATCGGTCGATCCAATATTGTCGGAAAACCTATGGCCCAGCTTCTTTTGGCA
AAGAATGCAACAGTAACCTTGACTCACTCACGTACTCATAATCTTTCCAAGGTGGCTGCA
AAAGCAGATATTCTGGTTGTTGCAATCGGTCGTGCCAAGTTTGTGACTGCTGACTTTGTC
AAACCAGGTGCGGTAGTCATTGACGTTGGGATGAACCGCGATGAAAATGGTAAGCTCTGT
GGGGATGTTGATTATGAGGCGGTTGCCCCACTTGCTAGCCACATTACGCCAGTCCCTGGA
GGTGTCGGTCCTATGACCATTACTATGCTGATGGAGCAAACCTATCAGGCAGCACTTAGG
ACATTGGATAGAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SP_0825 [new locus tag: SP_RS04035 ]
- symbol: FolD
- description: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
- length: 285
- theoretical pI: 6.5181
- theoretical MW: 30741.5
- GRAVY: 0.0435088
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED :
- Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)
- Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)
Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)and 4 moreCarbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) - PFAM: NADP_Rossmann (CL0063) THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 237.1)and 2 moreAA_dh_N (CL0603) THF_DHG_CYH; Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain (PF00763; HMM-score: 140.7)NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004291
- TAT(Tat/SPI): 0.000685
- LIPO(Sec/SPII): 0.000752
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNPASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: MtaR* (repression) regulon
MtaR* (TF) important in Methionine metabolism; RegPrecise
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0721 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Tim van Opijnen, Andrew Camilli
A fine scale phenotype-genotype virulence map of a bacterial pathogen.
Genome Res: 2012, 22(12);2541-51
[PubMed:22826510] [WorldCat.org] [DOI] (I p)