From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_1258 [new locus tag: SP_RS06170 ]
  • pan locus tag?: PNEUPAN002512000
  • symbol: SP_1258
  • pan gene symbol?: leuA
  • synonym:
  • product: putative 2-isopropylmalate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_1258 [new locus tag: SP_RS06170 ]
  • symbol: SP_1258
  • product: putative 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1190557..1191651
  • length: 1095
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACTTTCAATTAGCTAAATATTCTTTACTGAAGAGATTTTTAGAAAATATAGGATTT
    ACCACACTAGAGGAATATGGTGCCATCTTCAAATACCTGATTGAGAATGTCAAGACGGAT
    CGTCAGATCATCTATTCGCCTCACTGTCATGATGACCTCGGAATGGCAGTGGCAAATAGC
    CTTGCTGCTGTCAAGAATGGTGCAGGACGTGTTGAAGGGACTATCAATGGTATTAGGGAG
    CGAGCTGAAAATGCTGCTTTGGAAGAAATTGCAGTGGCTCTCAATATTCGCCAAGATTAC
    TACCAAGTAGAAACCAGTATTGTCCTAAATGAGACCATCAATACGTCAGAAATGGTTTCT
    CGCTTCTCTGGTATTCCAGTTCCTAAAAACAAAGCCGTCGTTGGTGGCAATACCTTCTCC
    CACGAATCTGGTATTCACCAAGATGGAGTCCTTAAAAATCCTCTCACTTATGAGATCATC
    ACACCTGAATTGGTTGGTGTTAAGATTCTGCTTGGAAAATTATCTGGTCGCCATGCTTTT
    GTTGAGAAACTGAGAGAATTGGCCCTAGATTTTACAGAAGAGGATATCAAACCACTCTTT
    GCTAAGTTCAAGGCACTGGTCGATAAGAAGCAAGAAATCACAGATGCAGATATTCGAGCT
    TTGGTAGCTGGAACCATGGTTGAAAATCCAGAAGGCTTCCACTTTGATGATTTACAACTT
    CAAACTCATGCAGATAATGACATTGAAGCGCTCGTTAGCCTAGCCAATATGGATGGTGAG
    AAAGTCGAATTTAATGCGACAGGGCAAGGTTCCGTTGAAGCAATCTTTAATGCTATCGAT
    AAGTTCTTTAACCAATCTGTTCGTTTGGTGTCCTACACTATCGATGCGGTAACAGATGGA
    ATCGATACCCAGGATCGGGTTTTGGTCACTGTTGAAAACAGAGATACAGAAACCATCTTT
    AATGCAGCAGGGCTTGATTTTGATGTGTTGAAGGCTTCTGCTATTGTCTATATAAACGCT
    AATACCTTTGTTCAAAAAGAGAATGCAGGTGAGATGGGACGCAGTGTTTCTTACCACGAT
    ATGCCTAGTGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1095

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_1258 [new locus tag: SP_RS06170 ]
  • symbol: SP_1258
  • description: putative 2-isopropylmalate synthase
  • length: 364
  • theoretical pI: 4.56183
  • theoretical MW: 40245.2
  • GRAVY: -0.122802

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.3.13?  ExPASy
    2-isopropylmalate synthase Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 350.7)
    and 8 more
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 199.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 164.4)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 164.4)
    Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 143.8)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 131.5)
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 63.5)
    Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 36.4)
    conserved hypothetical protein (TIGR02246; HMM-score: 14.2)
  • TheSEED  :
    • 2-isopropylmalate synthase (EC 2.3.3.13)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
    and 1 more
    Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
  • PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 96.9)
    no clan defined LeuA_dimer; LeuA allosteric (dimerisation) domain (PF08502; HMM-score: 96.9)
    and 2 more
    CDA (CL0109) Pput2613-deam; Pput_2613-like deaminase (PF14427; HMM-score: 13.6)
    no clan defined Phasin_2; Phasin protein (PF09361; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9
    • Cytoplasmic Membrane Score: 0.027
    • Cell wall & surface Score: 0.0026
    • Extracellular Score: 0.0704
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002646
    • TAT(Tat/SPI): 0.000236
    • LIPO(Sec/SPII): 0.000352
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEIITPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADIRALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP_1258&oldid=3478"