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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_1383 [new locus tag: SP_RS06780 ]
  • pan locus tag?: PNEUPAN002659000
  • symbol: alaS
  • pan gene symbol?: alaS
  • synonym:
  • product: alanyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_1383 [new locus tag: SP_RS06780 ]
  • symbol: alaS
  • product: alanyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1306327..1308945
  • length: 2619
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAACAACTATCTAGTGCACAAGTACGCCAAATGTGGCTTGATTTCTGGGCGACCAAA
    GGTCACTCAGTAGAACCATCAGTAAGTTTGGTTCCTGTAAATGACCCAACTCTTTTGTGG
    ATCAACTCTGGGGTAGCAACCCTTAAGAAATACTTTGACGGGACCATTATCCCTGAAAAT
    CCACGTATTACCAATGCCCAAAAGGCTATCCGTACCAACGACATCGAAAACGTAGGGAAG
    ACCGCGCGTCACCATACCATGTTTGAAATGCTGGGGAACTTCTCTATCGGGGATTACTTC
    CGTGACGAAGCTATCACTTGGGCTTATGAGCTTTTGACAAGCCCTGAATGGTTTGATTTC
    CCTGCTGAAAAACTTTACATGACCTACTATCCAGACGATAAAGATTCTTACAACCGCTGG
    ATTGAAGTGGGAGTGGATCCAAGTCACTTGATTCCAATTGAGGACAACTTCTGGGAAATC
    GGTGCGGGACCTTCTGGACCAGATACAGAAATCTTCTTTGACCGTGGGGAAGCCTTTGAC
    CCAGAAAATATCGGTCTTCGCCTGCTTGCAGAAGATATTGAAAACGACCGTTATATTGAA
    ATCTGGAACATCGTTTTGTCACAATTTAACGCAGACCCTGCTGTTCCTCGTAGCGAATAC
    AAGGAATTGCCACATAAGAACATTGATACGGGCGCTGGTTTGGAGCGTTTGGTGGCCGTT
    ATCCAAGGGGCTAAGACCAACTTTGAAACGGACCTCTTCATGCCGATTATCCGTGAAGTC
    GAGAAATTGTCTGGTAAGGTTTATGACCAAGATGGCGACAACATGAGCTTCAAGGTTATC
    GCAGACCACATTCGTTCTCTTTCATTTGCTATCGGTGATGGTGCCCTTCCAGGAAATGAA
    GGTCGTGGTTATGTTCTTCGTCGTCTTCTCCGTCGTGCTTCTATGCATGGTCAAAAATTG
    GGTATCAACGAGCCTTTCCTTTACAAATTGGTTCCAACTGTTGGAAAAATCATGGAAAGC
    TACTACCCAGAAGTGCTTGAGAAACGTGACTTTATTGAGAAAATCGTTAAGAGCGAAGAA
    GAATCATTTGCCCGTACCCTTCACTCAGGTCAACACTTTGCCCAAGGCATTGTAGCAGAC
    TTGAAAGAAAAAGGTCAATCTGTTATCGCTGGTTCAGATGTCTTCAAACTCTATGATACT
    TATGGGTTCCCAGTTGAATTGACTGAAGAAATCGCTGAAGAAGCTGGTATGACTGTAGAC
    CGTGAAGGTTTTGAAGCAGCCATGAAAGAACAGCAAGAACGCGCGCGTGCGTCAGCTGTC
    AAGGGTGGCTCAATGGGTATGCAAAATGAAACTCTTCAAAACATCACTGTAGAAAGTGTC
    TTCAACTACAATGCTAGCCAATTGTCTTCTAAATTGGTAGCTATCGTTGCTGACAATGCA
    GAAGTAGGAGCTGTTTCAGAAGGAACTGCCTCTCTTATCTTTGCGGAAACGTCATTTTAT
    GCTGAAATGGGTGGACAGGTAGCTGACTACGGACAAATCTTGGATGAGTCAGGTAAGGTT
    GTGGCTACTGTGACCAATGTTCAGAAAGCCCCAAATGGTCAAGCCCTTCATACAGTTGAA
    GTCCTTGCACCGCTTGCCTTGAACCAAGAATATACCTTGGCAATTGATAGCAATCGCCGT
    CACCGTGTCATGAAAAACCACACTGCGACTCATTTGCTTCACGCTGCCCTTCACAATATC
    CTTGGAAACCACGCAACACAGGCAGGATCTCTTAACGAAGTTGAATTCCTTCGCTTTGAC
    TTTACCCACTTCCAAGCAGTAACTGCTGAGGAACTTCGTGCGATTGAACAGCAAGTTAAC
    GAGAAAATTTGGGAAGCACTTGAAGTGAAGACAGTTGAAACGGATATTGACACTGCTAAA
    GAAATGGGAGCTATGGCCCTCTTTGGTGAGAAATACGGCAAGGAAGTTCGTGTCGTGACT
    ATCGGTGACTACTCTATTGAACTTTGTGGTGGTACTCATGTTGACAACACTTCTGAGATT
    GGTCTCTTCAAAATTGTCAAAGAAGAAGGAATCGGTTCAGGAACTCGCCGTATCTTGGCA
    GTGACTGGTAAGGAAGCCTTTGAAGCCTATCGTGAACAAGAGGATGCTCTTAAAGCTATC
    GCAGCAACCTTGAAAGCACCTCAAGTCAAGGAAGTACCTCACAAGGTAGAAGGACTTCAA
    GAACAACTTCGTCAACTTCAAAAAGAAAATGCTGAGTTGAAAGAAAAAGCCGCAGCTGCA
    GCCGCAGGCGATATCTTCAAAGATGTTAAGGAAGTCAACGGTCATCGTTACATTGCTAGT
    CAAGTGTCTGTATCCGATGCCGGTGCCCTTCGTACTTTTGCAGATAACTGGAAACAAAAA
    GACTACTCTGATCTTCTTGTCCTAGTTGCCGCTATCGGTGACAAAGTCAATGTCCTTGTA
    GCAAGCAAGACAAAAGACCTTCATGCAGGAAACCTTGTCAAAGAATTAGCACCAATCATC
    GATGGACGTGGTGGTGGTAAACCAGATATGGCCATGGCAGGAGGAAGCAACCAACCTAAG
    ATCCAAGAATTGTTGGATGCAGTAGCAGGTAAATTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2619

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_1383 [new locus tag: SP_RS06780 ]
  • symbol: AlaS
  • description: alanyl-tRNA synthetase
  • length: 872
  • theoretical pI: 4.79109
  • theoretical MW: 96439.2
  • GRAVY: -0.293693

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.7?  ExPASy
    Alanine--tRNA ligase ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation alanine--tRNA ligase (TIGR00344; EC 6.1.1.7; HMM-score: 961)
    and 2 more
    alanine--tRNA ligase (TIGR03683; EC 6.1.1.7; HMM-score: 404.1)
    Genetic information processing Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 27.5)
  • TheSEED  :
    • Alanyl-tRNA synthetase (EC 6.1.1.7)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Ala  Alanyl-tRNA synthetase (EC 6.1.1.7)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2c; tRNA synthetases class II (A) (PF01411; HMM-score: 601.6)
    and 11 more
    no clan defined DHHA1; DHHA1 domain (PF02272; HMM-score: 88.8)
    Peptidase_ME (CL0094) tRNA_SAD; Threonyl and Alanyl tRNA synthetase second additional domain (PF07973; HMM-score: 73.4)
    no clan defined DUF3659; Protein of unknown function (DUF3659) (PF12396; HMM-score: 16.8)
    FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 16.5)
    no clan defined HAP1_N; HAP1 N-terminal conserved region (PF04849; HMM-score: 16)
    DUF5660; Domain of unknown function (DUF5660) (PF18904; HMM-score: 13.4)
    VPS23_C (CL0596) Mod_r; Modifier of rudimentary (Mod(r)) protein (PF07200; HMM-score: 12.5)
    no clan defined UPF0242; Uncharacterised protein family (UPF0242) N-terminus (PF06785; HMM-score: 12.2)
    MCU; Mitochondrial calcium uniporter (PF04678; HMM-score: 8.8)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 8.1)
    no clan defined Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region (PF04871; HMM-score: 6.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9979
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.002
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017113
    • TAT(Tat/SPI): 0.000918
    • LIPO(Sec/SPII): 0.006704
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSGVATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRDEAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEIGAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMSFKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTVGKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGSDVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNETLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVADYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKNHTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIWEALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGLFKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQEQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWKQKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGSNQPKIQELLDAVAGKL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Ala) (transcription antitermination) regulon
    T-box(Ala)(RNA)important in Amino acid metabolism; RegPrecise 

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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