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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 23-APR-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_RS00655 [old locus tag: SP_0129 ]
  • pan locus tag?: PNEUPAN000805000
  • symbol: tsaD
  • pan gene symbol?: tsaD
  • synonym:
  • product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_RS00655 [old locus tag: SP_0129 ]
  • symbol: tsaD
  • product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD
  • replicon: chromosome
  • strand: +
  • coordinates: 129407..130417
  • length: 1011
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_003028 (129407..130417) NCBI
  • BioCyc:
  • MicrobesOnline: see SP_0129
  • PneumoBrowse for strain D39V: SPV_0136 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGGATAGATATATTTTAGCATTTGAGACATCCTGTGATGAGACCAGTGTCGCCGTC
    TTGAAAAACGACGATGAGCTCTTGTCCAATGTCATTGCTAGTCAAATTGAGAGTCACAAA
    CGTTTTGGTGGCGTAGTGCCCGAAGTAGCCAGTCGTCACCATGTCGAGGTCATTACAGCC
    TGTATCGAGGAGGCATTGGCAGAAGCAGGGATTACCGAAGAGGACGTGACAGCTGTTGCG
    GTTACCTACGGACCAGGCTTGGTCGGAGCCTTGCTAGTTGGTTTGTCAGCTGCCAAGGCC
    TTTGCTTGGGCTCACGGACTTCCACTGATTCCTGTTAATCACATGGCTGGGCACCTCATG
    GCAGCTCAGAGTGTGGAGCCTTTGGAGTTTCCCTTGCTAGCCCTCTTGGTCAGCGGCGGA
    CACACAGAGTTGGTTTATGTTTCGGAGGCAGGAGATTATAAGATTGTTGGGGAAACCCGT
    GATGATGCGGTTGGTGAGGCTTATGATAAGGTCGGCCGTGTCATGGGCTTGACCTATCCT
    GCAGGTCGTGAGATTGACGAGCTGGCTCATCAGGGGCAGGATATTTATGATTTCCCCCGT
    GCCATGATTAAGGAAGATAATCTGGAGTTCTCCTTCTCAGGTTTGAAATCTGCCTTTATC
    AATCTTCATCACAATGCCGAGCAAAAGGGAGAAAGCCTGTCTACAGAAGATTTGTGTGCT
    TCCTTCCAAGCAGCAGTTATGGACATTCTCATGGCAAAAACCAAGAAGGCTTTGGAGAAA
    TATCCTGTTAAAATCCTAGTTGTGGCAGGTGGTGTGGCAGCCAATAAAGGTCTCAGAGAA
    CGCCTAGCAGCCGAAATCACAGATGTCAAGGTTATCATCCCCCCTCTGCGACTCTGCGGA
    GACAATGCAGGTATGATTGCCTATGCCAGCGTCAGCGAGTGGAACAAAGAAAACTTCGCA
    GGCTGGGACCTCAATGCCAAACCAAGTCTTGCCTTTGATACCATGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_RS00655 [old locus tag: SP_0129 ]
  • symbol: TsaD
  • description: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD
  • length: 336
  • theoretical pI: 4.56594
  • theoretical MW: 36185.1
  • GRAVY: 0.0797619

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.234?  ExPASy
    N6-L-threonylcarbamoyladenine synthase L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N6-L-threonylcarbamoyladenine37 in tRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 430.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 350.7)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 207)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 63.8)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 14.8)
    Genetic information processing Protein fate Protein modification and repair carbamoyltransferase HypF (TIGR00143; HMM-score: 13.4)
  • TheSEED: see SP_0129
  • PFAM:
    Actin_ATPase (CL0108) TsaD; tRNA N6-adenosine threonylcarbamoyltransferase (PF00814; HMM-score: 348.9)
    and 2 more
    Carbam_trans_N; Carbamoyltransferase N-terminus (PF02543; HMM-score: 28.6)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 25.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9817
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0019
    • Extracellular Score: 0.014
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00337
    • TAT(Tat/SPI): 0.000656
    • LIPO(Sec/SPII): 0.000856
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGGVVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFAWAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETRDDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSAFINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKILVVAGGVAANKGLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDTME

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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