⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae Hungary19A-6
- locus tag: SPH_1749 [new locus tag: SPH_RS08505 ]
- pan locus tag?: PNEUPAN003028000
- symbol: SPH_1749
- pan gene symbol?: mntR
- synonym:
- product: iron-dependent transcriptional regulator
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPH_1749 [new locus tag: SPH_RS08505 ]
- symbol: SPH_1749
- product: iron-dependent transcriptional regulator
- replicon: chromosome
- strand: +
- coordinates: 1610492..1611142
- length: 651
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: CP000936 (1610492..1611142) NCBI
- BioCyc: see SPH_RS08505
- MicrobesOnline: 5696574 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_1450 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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601ATGACCCCAAACAAAGAAGACTATCTAAAATGTATTTATGAAATTGGCATAGACCTGCAT
AAGATTACCAACAAGGAAATTGCGGCTCGCATGCAAGTCTCTCCCCCTGCCGTAACTGAA
ATGATCAAACGAATGAAAAGTGAAAATCTCATCCTAAAGGACAAGGAATGTGGCTATCTA
CTGACTGACCTCGGTCTCAAACTGGTCTCTGAGCTCTATCGTAAGCACCGCTTGATTGAA
GTTTTTCTAGTTCATCATTTAGACTATACAAGTGACCAGATTCACGAGGAAGCTGAGGTC
TTGGAACACACTGTCTCTGACCTGTTCGTGGAAAGACTAGATAAACTGCTAGGTTTCCCT
AAAACCTGCCCCCACGGAGGAACTATTCCTGCCAAGGGAGAACTCCTCGTTGAAATCAAT
AACCTCCCACTAGCTGATATCAAGGAAGCTGGCGCCTACCGCCTGACTCGGGTGCACGAT
AGTTTTGACATTCTCCATTATCTGGACAAGCACTCACTTCACATCGGTGACCAGCTCCAA
GTCAAGCAGTTTGATGGCTTCAGCAATACCTTCACTATCCTCAGTAACGACGAGGATTTA
CAAGTGAATATGGACATTGCAAAACAACTCTATGTCGAGAAAATCAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPH_1749 [new locus tag: SPH_RS08505 ]
- symbol: SPH_1749
- description: iron-dependent transcriptional regulator
- length: 216
- theoretical pI: 5.74358
- theoretical MW: 24825.4
- GRAVY: -0.268519
⊟Function[edit | edit source]
- ⊞TIGRFAM: Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 16.4)phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 14.6)and 4 more
- TheSEED :
- Mn-dependent transcriptional regulator MntR
- ⊞PFAM: HTH (CL0123) Fe_dep_repr_C; Iron dependent repressor, metal binding and dimerisation domain (PF02742; HMM-score: 93.1)and 19 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effector: Manganese ion, (Mn2+)
- ⊞genes regulated by MntR*, TF important in Manganese homeostasisRegPrecise
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKRMKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLEHTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHDSFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1450 PneumoExpress
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Jason W Johnston, David E Briles, Lisa E Myers, Susan K Hollingshead
Mn2+-dependent regulation of multiple genes in Streptococcus pneumoniae through PsaR and the resultant impact on virulence.
Infect Immun: 2006, 74(2);1171-80
[PubMed:16428766] [WorldCat.org] [DOI] (P p)Tomas G Kloosterman, Robert M Witwicki, Magdalena M van der Kooi-Pol, Jetta J E Bijlsma, Oscar P Kuipers
Opposite effects of Mn2+ and Zn2+ on PsaR-mediated expression of the virulence genes pcpA, prtA, and psaBCA of Streptococcus pneumoniae.
J Bacteriol: 2008, 190(15);5382-93
[PubMed:18515418] [WorldCat.org] [DOI] (I p)Wouter T Hendriksen, Hester J Bootsma, Angela van Diepen, Silvia Estevão, Oscar P Kuipers, Ronald de Groot, Peter W M Hermans
Strain-specific impact of PsaR of Streptococcus pneumoniae on global gene expression and virulence.
Microbiology (Reading): 2009, 155(Pt 5);1569-1579
[PubMed:19372167] [WorldCat.org] [DOI] (P p)Radha Gupta, Minny Bhatty, Edwin Swiatlo, Bindu Nanduri
Role of an iron-dependent transcriptional regulator in the pathogenesis and host response to infection with Streptococcus pneumoniae.
PLoS One: 2013, 8(2);e55157
[PubMed:23437050] [WorldCat.org] [DOI] (I p)Irfan Manzoor, Sulman Shafeeq, Oscar P Kuipers
Ni2+-Dependent and PsaR-Mediated Regulation of the Virulence Genes pcpA, psaBCA, and prtA in Streptococcus pneumoniae.
PLoS One: 2015, 10(11);e0142839
[PubMed:26562538] [WorldCat.org] [DOI] (I e)