⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 05-OCT-2017

⊟Summary

• organism: Streptococcus pneumoniae MDRSPN001
locus tag: MDRSPN_01831 [new locus tag: MDRSPN_RS09635 ]
pan locus tag^?: PNEUPAN003625000
symbol: MDRSPN_01831
pan gene symbol^?: ulaR
• synonym:
• product: L-ascorbate-6-phosphate lactonase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: MDRSPN_01831 [new locus tag: MDRSPN_RS09635 ]
• symbol: MDRSPN_01831
• product: L-ascorbate-6-phosphate lactonase
• replicon: chromosome
• strand: -
• coordinates: 1818096..1819769
• length: 1674
• essential: unknown

⊟Accession numbers

• Location: AP018391 (1818096..1819769) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1841 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  
  ATGGTACTGGATAAGGCAAGTTGTGATTTGCTTCAATATTTGATGGATCAAGAAACGTCC
  AAAACGATTATGGCGATTTCGAAAGATTTGAAAGAGTCAAGAAGGAAAATTTATTATCAC
  ATTGACAAAATCAATGCTGCTCTGGGTGACGAGGCGCTTCACATCATTAGTATTCCACGA
  ATTGGTATTCACTTAACGGAAGAGCAGAGAAATGCTTGTTGTGAACTATTATCGGAAGTA
  GATTCGTACGATTATATCATGAGTGCTCATGAACGTATGATGATAATGTTACTATGGATA
  GGTATTTCTAAAGAACGTATTACGATTGAAAAATTGATGGAGTTAACAGAGGTGTCTAGG
  AATACTGTTCTCAATGATTTGAATAGTATTCGTTATCAACTAACTTTGGAACAATATCAG
  GTGACCTTGCAAGTGAGCAAGTCACAGGGATACCATCTTCATGCCCACCCTCTTAATAAA
  ATTCAGTATCTTCAATCGCTTCTATATCATATTTTTATGGAGGAAAATGCCACTTTTGTA
  TCTATTTTAGAAGATAAGATGAAAGAGAGGTTAGATGGTGAGTGTTTGCTTTCTGTTGAA
  ATGAACCAATTTTTTAAGGAACAGGTTCCTTTAGTTGAACAAGATTTAGGGAAGAAAATA
  AACCATCATGAAGTAACTTTTATGTTGCAGGTTCTACCTTATTTGCTGTTAAGCTGTCAT
  AATGTTGAACAGTATCAAGAAAGACATCAGGATATAGAGAAAGAATTTTCTTTGATAAGA
  AAAAGAATAGAGTATCAGGTGTCTAAGAAATTAGGAGAACGATTGTTTCAAAAGTTTGAA
  ATTTCTTTGTCAGAACTTGAAGTTTCTCTTGTAGCTGTTCTTCTCCTCTCCTATCGTAAA
  GATTTGGATATTCATGCAGAAAGTGATGATTTTCGGCAATTAAAACTTGCTTTAGAAGAA
  TTTATCTGGTATTTTGAATCACAAATCCGAATGGAGATTGAGAACAAGGATGATTTGTTA
  AGAAATTTGATGATCCACTGTAAAGCCTTGTTATTTAGAAAGACTTACGGTATTTTTTCT
  AAAAATCCTCTAACAAAACAAATTCGATCCAAGTATGGAGAATTATTTTTAGTCACTAGA
  AAATCTGCGGAAATTTTAGAAGGAGCATGGTTTATTCGGCTAACAGATGATGATATTGCC
  TATTTGACGATTCATATTGGAGGATTTTTAAAGTATACACCATCGTCTCAAAAAAATATG
  AAAAAAGTTTATCTCGTTTGTGATGAAGGTGTTGCGGTTTCGAGACTTTTGCTGAAACAA
  TGCAAACTTTATTTTCCAAATGAGCAAATTGGCACTGTATTTACAACAGAACAATTTAAG
  AGTGTGGAAGATATTGCACAAGTTGATGTAGTGATTACTACTAATGATGATTTGGATAGC
  AGATTTCCGATTTTAAGGGTTAATCCTATCCTTGAAGCAGAAGATATTTTGAAAATGCTA
  GACTATCTTAAACACAATATATTTCGTAATGAGAGCAAAAGTTTCAGTGAAAATCTTTCT
  AGTCTTATTTCGTCTTATATTGTAGACAGCAAGTTGGCTAGTAAGTTTCAAGAAGAGGTT
  CAAACACTTATAAATCAAGAAATAGTAGTTCAAGCTTTTTTGGAAGATATTTGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1674
  

⊟Protein

⊟General

• locus tag: MDRSPN_01831 [new locus tag: MDRSPN_RS09635 ]
• symbol: MDRSPN_01831
• description: L-ascorbate-6-phosphate lactonase
• length: 557
• theoretical pI: 5.42918
• theoretical MW: 65269.1
• GRAVY: -0.183303

⊟Function

• TIGRFAM:
  Genetic information processing Transcription Transcription factors transcription termination factor NusA, C-terminal duplication (TIGR01954; HMM-score: 12.9)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 96.4)
  and 6 more
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 36.6)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 17)
  Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 14)
  HTH (CL0123) HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 14)
  no clan defined BTRD1; Bacterial tandem repeat domain 1 (PF17660; HMM-score: 12)
  RNA_binding; RNA binding (PF01877; HMM-score: 7.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by UlaR*, TF important in Ascorbate utilization: in TIGR4

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00091
  • TAT(Tat/SPI): 0.000421
  • LIPO(Sec/SPII): 0.000355
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: BBA59991 NCBI
• UniProt:

⊟Protein sequence

• MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKINAALGDEALHIISIPRIGIHLTEEQRNACCELLSEVDSYDYIMSAHERMMIMLLWIGISKERITIEKLMELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNKIQYLQSLLYHIFMEENATFVSILEDKMKERLDGECLLSVEMNQFFKEQVPLVEQDLGKKINHHEVTFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLFQKFEISLSELEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIENKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFIRLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQIGTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIFRNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1841 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Muhammad Afzal, Sulman Shafeeq, Birgitta Henriques-Normark, Oscar P Kuipers
UlaR activates expression of the ula operon in Streptococcus pneumoniae in the presence of ascorbic acid.
Microbiology (Reading): 2015, 161(Pt 1);41-49
[PubMed:25355938] [WorldCat.org] [DOI] (I p)Sulman Shafeeq, Muhammad Afzal, Birgitta Henriques-Normark, Oscar P Kuipers
Transcriptional profiling of UlaR-regulated genes in Streptococcus pneumoniae.
Genom Data: 2015, 4;57-9
[PubMed:26484177] [WorldCat.org] [DOI] (P e)