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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_1427 [new locus tag: SPD_RS07585 ]
  • pan locus tag?: PNEUPAN003003000
  • symbol: phnA
  • pan gene symbol?: phnA
  • synonym:
  • product: PhnA protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_1427 [new locus tag: SPD_RS07585 ]
  • symbol: phnA
  • product: PhnA protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1445359..1445697
  • length: 339
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP000410 (1445359..1445697) NCBI
  • BioCyc: see SPD_RS07585
  • MicrobesOnline: 2018313 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_1427 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGAACAATTTACCAAATTGTCCAAAATGTAACTCAGAGTATGTCTACGAAGACGGTGCC
    CTACTGGTTTGCCCAGAGTGTGCTCATGAGTGGAATCCTGCTGAAGTTGCAGAAGTAGAA
    GAAGGTCTTGTCGCTATCGATGCCAACGGAAATAAATTGACTGATGGTGATACAGTAACT
    CTCATCAAGGACTTGAAAGTAAAAGGTGCGCCAAAAGATTTGAAACAAGGGACGCGCGTG
    AAAAATATCCGCATCGTAGAAGGCGATCACAATATCGACTGTAAGATTGATGGTTTTGGT
    GCCATGAAACTTAAATCAGAGTTTGTGAGGAAGATTTAA
    60
    120
    180
    240
    300
    339

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_1427 [new locus tag: SPD_RS07585 ]
  • symbol: PhnA
  • description: PhnA protein
  • length: 112
  • theoretical pI: 4.9408
  • theoretical MW: 12314
  • GRAVY: -0.390179

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Unknown function General putative alkylphosphonate utilization operon protein PhnA (TIGR00686; HMM-score: 166.7)
    and 3 more
    flagellar operon protein TIGR03826 (TIGR03826; HMM-score: 17.2)
    Genetic information processing Transcription Transcription factors transcription factor S (TIGR01384; HMM-score: 15.6)
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 8.4)
  • TheSEED  :
    • Protein PhnA
  • ⊞PFAM:
    SH3 (CL0010) YjdM; PhnA domain (PF03831; HMM-score: 111.1)
    and 16 more
    Zn_Beta_Ribbon (CL0167) YjdM_Zn_Ribbon; PhnA Zinc-Ribbon (PF08274; HMM-score: 59.7)
    TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 17.3)
    no clan defined FtrD-like; Membrane iron-sulfur containing protein FtrD-like (PF10080; HMM-score: 17.1)
    Zn_Beta_Ribbon (CL0167) A2L_zn_ribbon; A2L zinc ribbon domain (PF08792; HMM-score: 16.2)
    zf-RRN7; Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7 (PF11781; HMM-score: 14.9)
    zf-TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 14.8)
    zinc_ribbon_4; zinc-ribbon domain (PF13717; HMM-score: 13.1)
    zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 12.9)
    OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 12.9)
    HeH (CL0306) PADR1; PADR1 (NUC008) domain (PF08063; HMM-score: 12.6)
    Zn_Beta_Ribbon (CL0167) UPF0547; Uncharacterised protein family UPF0547 (PF10571; HMM-score: 12.5)
    TFIIS_C; Transcription factor S-II (TFIIS) (PF01096; HMM-score: 11.7)
    no clan defined FYDLN_acid; Protein of unknown function (FYDLN_acid) (PF09538; HMM-score: 11.3)
    Zn_Beta_Ribbon (CL0167) Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 11)
    Zn_Tnp_IS1; InsA N-terminal domain (PF03811; HMM-score: 10.4)
    Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013035
    • TAT(Tat/SPI): 0.000275
    • LIPO(Sec/SPII): 0.002127
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ABJ54682 NCBI
  • UniProt: A0A0H2ZPR0 UniProt

⊟Protein sequence[edit | edit source]

  • MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLVAIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAMKLKSEFVRKI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SPD_1426 < phnA

⊟Regulation[edit | edit source]

  • ⊞regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1427 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPD_1427&oldid=8010"
  • This page was last edited on 20 January 2022, at 13:22.
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