⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 25-OCT-2019

⊟Summary

• organism: Streptococcus pneumoniae NU83127
locus tag: SPNU_0491 [new locus tag: EL277_RS02610 ]
pan locus tag^?: PNEUPAN001402000
symbol: hrcA
pan gene symbol^?: hrcA
• synonym:
• product: Heat-inducible transcription repressor HrcA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNU_0491 [new locus tag: EL277_RS02610 ]
• symbol: hrcA
• product: Heat-inducible transcription repressor HrcA
• replicon: chromosome
• strand: +
• coordinates: 500995..502029
• length: 1035
• essential: unknown

⊟Accession numbers

• Location: AP018936 (500995..502029) NCBI
• BioCyc: see EL277_RS02610
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0458 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGGTTACAGAGCGTCAGCAGGATATTTTAAATCTGATTATTGACATCTTTACCAAAACG
  CACGAACCTGTCGGATCAAAAGCCTTGCAAGAGTCTATTAACTCTAGCAGTGCAACCATT
  CGTAATGACATGGCGGAACTAGAAAAACAAGGGTTGCTTGAGAAGGCTCATACTTCAAGC
  GGTCGGATGCCAAGTGTTGCTGGTTTTCAGTACTATGTGAAACACTCACTGGATTTTGAC
  CGGCTGGCTGAAAATGAGGTATATGAGATTGTCAAAGCCTTTGATCAGGAATTCTTCAAA
  TTGGAGGATATTCTGCAAGAGGCTGCTAACTTACTAACAGACCTGAGTGGCTGTACGGTA
  GTGGCACTGGATGTTGAGCCGAGCAGGCAACGTTTGACAGCCTTTGATATCGTTGTTTTG
  GGGCAACATACAGCCTTGGCGGTATTTACCCTAGACGAGTCGCGAACGGTTACTAGTCAG
  TTTCTGATTCCAAGGAACTTCTTGCAGGAGGATTTGCTGAAACTGAAGAGCATCATTCAG
  GAACGTTTCCTCGGTCACACCGTTTTAGATATTCACTACAAGATTCGGACGGAGATTCCG
  CAGATTATCCAGCGTTACTTTACAACAACGGATAATGTCATCGATCTCTTTGAACACATC
  TTTAAGGAAATGTTCAACGAAAACATTGTGATGGCGGGCAAGGTCCATCTCTTGAATTTT
  GCCAATCTAGCAGCCTATCAGTTCTTTGACCAACCGCAAAAGGTGGCCTTGGAGATTCGT
  GAGGGGTTGCGTGAGGATCAGATGCAAAATGTTCGTGTTGCAGACGGTCAAGAGTCCTGT
  TTAGCTGACCTAGCGGTAATCAGTAGTAAGTTCCTCATTCCTTATCGGGGAGTTGGAATT
  CTAGCCATTATCGGTCCAGTTAATCTGGATTACCAACAGCTAATCAATCAAGTCAATGTG
  GTCAACCGTGTTTTGACCATGAAGTTGACAGATTTTTACCGCTACCTCAGCAGTAATCAT
  TACGAAGTACATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1035
  

⊟Protein

⊟General

• locus tag: SPNU_0491 [new locus tag: EL277_RS02610 ]
• symbol: HrcA
• description: Heat-inducible transcription repressor HrcA
• length: 344
• theoretical pI: 5.00731
• theoretical MW: 39376.8
• GRAVY: -0.069186

⊟Function

• ⊞⊞TIGRFAM:
  Signal transduction Regulatory functions DNA interactions heat-inducible transcription repressor HrcA (TIGR00331; HMM-score: 314.3)
  and 3 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 19.3)
  Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 19.3)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.6)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 107.7)
  and 9 more
  HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 29.4)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 27.1)
  HTH_11; HTH domain (PF08279; HMM-score: 24.1)
  LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 18.5)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 16.3)
  HTH_12; Ribonuclease R winged-helix domain (PF08461; HMM-score: 16.3)
  Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 13.3)
  FUR; Ferric uptake regulator family (PF01475; HMM-score: 13)
  MarR; MarR family (PF01047; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by HrcA, TF important in Heat shock response: in TIGR4

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011174
  • TAT(Tat/SPI): 0.002104
  • LIPO(Sec/SPII): 0.001008
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: BBG34382 NCBI
• UniProt:

⊟Protein sequence

• MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDMAELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLEDILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQFLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFEHIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADGQESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYRYLSSNHYEVH

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_0458 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Su-Nam Kim, Yong-Goo Bae, Dong-Kwon Rhee
Dual regulation of dnaK and groE operons by HrcA and Ca++ in Streptococcus pneumoniae.
Arch Pharm Res: 2008, 31(4);462-7
[PubMed:18449503] [WorldCat.org] [DOI] (P p)