⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 17-DEC-2017

⊟Summary

• organism: Streptococcus pneumoniae Xen35
locus tag: CXP32_04895 [new locus tag: CXP32_RS04895 ]
pan locus tag^?: PNEUPAN001979000
symbol: CXP32_04895
pan gene symbol^?: trmR
• synonym:
• product: O-methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: CXP32_04895 [new locus tag: CXP32_RS04895 ]
• symbol: CXP32_04895
• product: O-methyltransferase
• replicon: chromosome
• strand: +
• coordinates: 926335..927048
• length: 714
• essential: unknown

⊟Accession numbers

• Location: CP025256 (926335..927048) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0867 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGTTGAATCGTATAGTAAGAATGCTAACCATAACATGCGTCGTCCTGTCGTCAAAGAA
  GAAATTGTAGACTTGATGCGTCAGCGTCAAAAGCAGGTCACAGGTTTCTTGAAAGAATTG
  GAAGACTTTGCCCGCAAGGAAAATATTCCTATTATTCCCCATGAAACGGTTGCTTATTTC
  CGTTTTCTTATGGAAACCATGCAGCCTAAAAATATTCTGGAAATTGGGACGGCTATCGGT
  TTTTCAGCTCTCTTGATGGCTGAACATGCGCCAAATGCTAAGATTACAACTATTGATCGT
  AATCCAGAAATGATTGGTTTTGCCAAGGAAAATTTTGCCCAGTTTGACAGTCGCAAGCAA
  ATCACTCTCCTAGAGGGAGATGCGGTGGATGTCTTATCTACACTGACAGAGTCTTATGAT
  TTCGTCTTTATGGATTCTGCCAAGTCTAAATACATCGTCTTTCTGCCAGAAATCCTCAAA
  CATTTGGAAGTTGGTGGTGTGGTTGTCTTGGATGATATTTTTCAAGGTGGTGATGTTGCC
  AAGGATATTATGGAAGTCCGTCGTGGTCAGCGAACCATTTATCGAGGCCTTCAAAAATTA
  TTTGATGCAACCTTAGACAATCCAGAACTCACCGCAACATTAGTGCCTTTAGGAGATGGT
  ATTCTCATGCTTCGTAAAAATGTAGCAGATGTTCAACTGTCTGAAAGCGAATGA
  60
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  714
  

⊟Protein

⊟General

• locus tag: CXP32_04895 [new locus tag: CXP32_RS04895 ]
• symbol: CXP32_04895
• description: O-methyltransferase
• length: 237
• theoretical pI: 4.91611
• theoretical MW: 26867.8
• GRAVY: -0.157384

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 46.8)
  and 16 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 31.7)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 29.5)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 23.8)
  Unknown function Enzymes of unknown specificity tRNA (cmo5U34)-methyltransferase (TIGR00740; EC 2.1.1.-; HMM-score: 23.2)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 22.7)
  methyltransferase, ATP-grasp peptide maturase system (TIGR04188; HMM-score: 21.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 20.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 20.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 19.9)
  methyltransferase, FxLD system (TIGR04364; HMM-score: 18.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 18.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 17.8)
  methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 17.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 16.6)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 15.8)
  methyltransferase, FkbM family (TIGR01444; HMM-score: 15.8)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 102.6)
  and 19 more
  Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 49)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 45.8)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 37.7)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 30.5)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 27.5)
  Met_10; Met-10+ like-protein (PF02475; HMM-score: 24.5)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 24)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 23.9)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 22.8)
  Spermine_synth; Spermine/spermidine synthase domain (PF01564; HMM-score: 21.3)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 19.1)
  Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 17.3)
  CmcI; Cephalosporin hydroxylase (PF04989; HMM-score: 16.8)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 15.7)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 15.3)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 13.9)
  Methyltransf_5; MraW methylase family (PF01795; HMM-score: 13.4)
  Rsm22; Mitochondrial small ribosomal subunit Rsm22 (PF09243; HMM-score: 13.4)
  ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9992
  • Cytoplasmic Membrane Score: 0.0003
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0003
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002076
  • TAT(Tat/SPI): 0.000283
  • LIPO(Sec/SPII): 0.000321
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: AUF84767 NCBI
• UniProt:

⊟Protein sequence

• MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFARKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNPEMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILKHLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLGDGILMLRKNVADVQLSESE

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for D39, D39V

⊟Expression data

• PneumoExpress for strain D39V: SPV_0867 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications