⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 16-DEC-2024

⊟Summary

• organism: Streptococcus pneumoniae CGSP14
locus tag: SPCG_RS08835 [old locus tag: SPCG_1707 ]
pan locus tag^?: PNEUPAN003157000
symbol: rsmB
pan gene symbol^?: sun
• synonym:
• product: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPCG_RS08835 [old locus tag: SPCG_1707 ]
• symbol: rsmB
• product: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB
• replicon: chromosome
• strand: -
• coordinates: 1680148..1681461
• length: 1314
• essential: unknown

⊟Accession numbers

• Location: NC_010582 (1680148..1681461) NCBI
• BioCyc: SPCG_RS08835 BioCyc
• MicrobesOnline: see SPCG_1707
• PneumoBrowse for strain D39V: SPV_1544 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  GTGACTAAAGTAGAAACGGCTAGAAGTTTAGCTCTAGCAGTGCTAGAGGATGTTTTTGTG
  AACCAAGCATATTCAAATATCGCCTTAAATAAACACCTCAAGGGGAGTCAGCTCTCTGCA
  GCAGACAAGGGCTTAGTGACCGAGCTAGTCTATGGAACGGTAGCCCGTAAACTGACTCTG
  GAATGGTACCTATCCCACTTTATCGAAGACAGAGACCAGTTAGACAGCTGGCTCTATGTC
  CTTCTTCTCATGAGTGTCTACCAACTCCGCTATTTGGACAAGATTCCAGATCATGCTGTG
  GTCAATGAAGCAGTGGAATTGGCCAAACTCCGTAAAAAAGGCAGTGAAAAATTGGTCAAC
  GCTGTCCTTCGCCGTATCTTGCGTGAAGGCTGGCCAGATATTGCTAACATCAAGCGAAAA
  AACAAGCGTGACTCCATTGCCTATTCTCTCCCAGTTTGGCTAGTTACCAAACTCAAGGAA
  GAATACGGAGAGGAGAGAGCAAAAGCCATCTTTGAAAGCTTGTTAGTGCGAAACAAAGCT
  AGTATTCGTGTAACAGACCTAAGTCGAAAAGAGGAAATCCAAGCCTTGTTGGAGGCGAAT
  AGTTCCCTTTTAGCAACCACTGGTCTGGTTAAGGAGCAAGGGCATTTTGCAGGGCATAAT
  TTGTTTGCGGATGGAGCCATTACCATCCAAGATGAGTCCAGTCAGCTGGTTGCTCCGACG
  CTTGATTTACAAGGTGATGAGCGAGTGCTTGATGCCTGTGCGGCTCCAGGTGGGAAAACA
  GCCCATATAGCCTCTTATCTTACGACAGGTCAGGTTACTGCTCTGGACTTGTACGACCAC
  AAGTTGGATTTAATTCAAGAAAATGCCCAACGTCTGGGAGTTGCAGATCGGGTTCAAACT
  CAAAAATTGGATGCCAGAAAGGTGCATGAGTTTTTTGACCAGGATTCCTTTGATAAGATT
  TTGGTGGATGCTCCTTGTTCAGGAATCGGTCTTTTGCGCCGAAAACCAGATATCAAATAC
  AATAAAGAAACGGCAGATTTCGCGTCCTTGCAGGAAATTCAGTTAGAAATATTAGGTAGT
  GTTTGTCAAATACTAGGCAAAGGTGGTATAATAACTTATAGCACCTGCACTATCGTCTCA
  GAGGAGAATTTTCAAGTCGTTAAGGCCTTTTTAGAGAGTCATCCCGAGTTCGAGCAGGTA
  AAACTAGAACATGAATGTAAGGATATCATGAAAGACGGCTGTATCCTCATTACACCTGAA
  TTGTATGGAAGTGATGGATTCTTCATCAGTCAATTTCGCAAGATATCGGATTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1314
  

⊟Protein

⊟General

• locus tag: SPCG_RS08835 [old locus tag: SPCG_1707 ]
• symbol: RsmB
• description: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB
• length: 437
• theoretical pI: 5.8625
• theoretical MW: 48998.8
• GRAVY: -0.169794

⊟Function

• ⊞⊞reaction:
  EC 2.1.1.176?  ExPASy
  16S rRNA (cytosine⁹⁶⁷-C₅)-methyltransferase S-adenosyl-L-methionine + cytosine⁹⁶⁷ in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine⁹⁶⁷ in 16S rRNA
• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 380)
  and 10 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification NOL1/NOP2/sun family putative RNA methylase (TIGR00446; EC 2.1.1.-; HMM-score: 146.8)
  Genetic information processing Transcription Transcription factors transcription antitermination factor NusB (TIGR01951; HMM-score: 53.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 25.5)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 23.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 22.9)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 19.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 17.4)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 16.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll C-20 methyltransferase BchU (TIGR02716; EC 2.1.1.-; HMM-score: 13.1)
  ribose 5-phosphate isomerase (TIGR02133; EC 5.3.1.6; HMM-score: 11.9)
• TheSEED: see SPCG_1707
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 182.5)
  and 13 more
  NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 100.4)
  NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 31.5)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.8)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 26.5)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 26.1)
  Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 19.6)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 18.3)
  Met_10; Met-10+ like-protein (PF02475; HMM-score: 18)
  Methyltr_RsmF_N; N-terminal domain of 16S rRNA methyltransferase RsmF (PF17125; HMM-score: 15.1)
  TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 14.9)
  Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 13.9)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 12.9)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9929
  • Cytoplasmic Membrane Score: 0.0026
  • Cell wall & surface Score: 0.0028
  • Extracellular Score: 0.0017
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00902
  • TAT(Tat/SPI): 0.001032
  • LIPO(Sec/SPII): 0.000968
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001815350 NCBI
• UniProt:

⊟Protein sequence

• MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKGLVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSVYQLRYLDKIPDHAVVNEAVELAKLRKKGSEKLVNAVLRRILREGWPDIANIKRKNKRDSIAYSLPVWLVTKLKEEYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANSSLLATTGLVKEQGHFAGHNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALDLYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRRKPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLESHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: stkP < SPCG_RS08830 < rsmB < fmt < SPCG_RS08845 < rpoZ < gmk

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1544 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications