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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 17-JAN-2018

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_1206 [new locus tag: SPAP_RS05960 ]
  • pan locus tag?: PNEUPAN002417000
  • symbol: SPAP_1206
  • pan gene symbol?: ptsI
  • synonym: ptsP
  • product: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_1206 [new locus tag: SPAP_RS05960 ]
  • symbol: SPAP_1206
  • product: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
  • replicon: chromosome
  • strand: -
  • coordinates: 1144806..1146530
  • length: 1725
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP002121 (1144806..1146530) NCBI
  • BioCyc: see SPAP_RS05960
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1039 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTTAAAGGAATCGCAGCATCTGACGGTGTTGCAGTTGCAAAAGCATATCTACTCGTT
    CAGCCGGATTTGTCATTTGAGACTATTACAGTCGAAGATACAAACGCAGAAGAAGCTCGC
    CTTGATGCCGCTCTACAGGCATCACAAGACGAGCTTTCTGTTATTCGCGAGAAAGCAGTA
    GGTACGCTCGGTGAAGAAGCAGCTCAAGTTTTTGATGCTCACTTAATGGTTCTTGCTGAC
    CCAGAAATGATCAGCCAAATCAAGGAAACTATCCGTGCGAAGAAAGTGAATGCAGAAGCA
    GGTCTGAAAGAAGTTACAGATATGTTTATCACTATCTTTGAAGGCATGGAAGACAACCCA
    TACATGCAAGAACGCGCAGCGGATATCCGCGATGTGACAAAACGTGTATTGGCAAACCTT
    CTTGGTAAAAAATTGCCAAACCCAGCTTCTATCAATGAAGAAGTGATTGTGATTGCACAT
    GACTTGACACCATCTGATACAGCTCAATTGGACAAAAACTTTGTAAAAGCTTTTGTAACC
    AACATCGGTGGACGTACAAGCCACTCAGCTATCATGGCACGTACACTTGAAATTGCAGCT
    GTATTAGGTACAAACAACATCACTGAAATCGTTAAAGACGGTGACATCCTTGCTGTTAAC
    GGGATCACTGGTGAGGTTATTATCAACCCAACTGATGAACAAGCGGCAGAATTTAAAGCA
    GCTGGTGAAGCTTATGCGAAACAAAAAGCTGAATGGGCGCTCTTGAAAGATGCTCAAACA
    GTGACTGCTGACGGTAAACACTTCGAGTTGGCTGCTAATATCGGTACTCCAAAAGACGTT
    GAAGGTGTTAACAACAACGGTGCAGAAGCTGTTGGACTTTACCGTACAGAGTTCTTGTAC
    ATGGATTCTCAAGACTTCCCAACTGAAGATGAGCAGTATGAAGCATACAAGGCTGTTCTT
    GAAGGAATGAACGGTAAACCTGTTGTCGTTCGTACAATGGATATCGGTGGAGATAAGGAA
    CTTCCTTACTTCGATATGCCTCACGAAATGAACCCATTCCTTGGATTCCGTGCTCTTCGT
    ATCTCTATCTCTGAGACTGGAGATGCTATGTTCCGCACACAAATCCGTGCTCTTCTTCGT
    GCGTCTGTTCACGGTCAATTGCGTATCATGTTCCCAATGGTTGCGCTCTTGAAAGAATTC
    CGTGCAGCGAAAGCAGTCTTTGATGAAGAAAAAGCAAACCTTCTTGCTGAAGGTGTTGCA
    GTTGCGGATAACATCCAAGTTGGTATCATGATCGAGATTCCTGCAGCGGCTATGCTTGCA
    GACCAATTTGCTAAAGAAGTTGACTTCTTCTCAATTGGTACAAACGACTTGATCCAATAT
    ACAATGGCAGCAGACCGTATGAACGAACAAGTTTCATACCTTTACCAACCATACAACCCA
    TCAATCCTACGCTTGATTAACAATGTGATCAAAGCAGCTCACGCTGAAGGTAAATGGGCT
    GGTATGTGTGGTGAGATGGCTGGTGACCAACAAGCTGTTCCACTTCTTGTCGGAATGGGC
    TTGGATGAGTTCTCTATGTCAGCAACATCTGTACTTCGTACACGCAGCTTGATGAAGAAA
    CTCGACACAGCTAAGATGGAAGAGTACGCAAACCGTGCCCTTACAGAATGCTCAACAATG
    GAAGAAGTTCTTGAACTTCAAAAAGAATACGTTAATTTTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1725

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_1206 [new locus tag: SPAP_RS05960 ]
  • symbol: SPAP_1206
  • description: Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)
  • length: 574
  • theoretical pI: 4.36148
  • theoretical MW: 62934.5
  • GRAVY: -0.0902439

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 751.5)
    and 4 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 203.3)
    Metabolism Energy metabolism Other pyruvate, phosphate dikinase (TIGR01828; EC 2.7.9.1; HMM-score: 132.9)
    Genetic information processing Transcription RNA processing 16S rRNA processing protein RimM (TIGR02273; HMM-score: 14.6)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 10.1)
  • TheSEED  :
    • Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)
    Carbohydrates Monosaccharides Fructose utilization  Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)
  • ⊞PFAM:
    PK_TIM (CL0151) PEP-utilizers_C; PEP-utilising enzyme, PEP-binding domain (PF02896; HMM-score: 424.4)
    and 3 more
    no clan defined PEP-utilisers_N; PEP-utilising enzyme, N-terminal (PF05524; HMM-score: 108.8)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 78.8)
    B_Fructosidase (CL0143) Glyco_hydro_62; Glycosyl hydrolase family 62 (PF03664; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008762
    • TAT(Tat/SPI): 0.000608
    • LIPO(Sec/SPII): 0.001802
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM84794 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAEAGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIVIAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGDILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEVLELQKEYVNFD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1039 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPAP_1206&oldid=43398"
  • This page was last edited on 20 January 2022, at 14:43.
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