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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-OCT-2019

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae NU83127
  • locus tag: SPNU_1482 [new locus tag: EL277_RS07800 ]
  • pan locus tag?: PNEUPAN002961000
  • symbol: engB
  • pan gene symbol?: engB
  • synonym: yihA
  • product: putative GTP-binding protein EngB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNU_1482 [new locus tag: EL277_RS07800 ]
  • symbol: engB
  • product: putative GTP-binding protein EngB
  • replicon: chromosome
  • strand: -
  • coordinates: 1472118..1472705
  • length: 588
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: AP018936 (1472118..1472705) NCBI
  • BioCyc: see EL277_RS07800
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1398 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGAACTTAATACACACAATGCTGAAATCTTGCTCAGTGCAGCTAATAAGTCCCACTAT
    CCGCAGGATGAACTGCCAGAGATTGCCCTAGCAGGGCGTTCAAATGTTGGTAAATCCAGC
    TTTATCAACACTATGTTGAACCGTAAGAATCTCGCCCGTACATCAGGAAAACCTGGTAAA
    ACCCAGCTCCTGAACTTTTTTAACATTGATGACAAGATGCGCTTTGTGGATGTGCCTGGT
    TATGGCTATGCTCGTGTTTCTAAAAAGGAACGTGAAAAGTGGGGGTGCATGATTGAGGAG
    TACTTAACGACTCGGGAAAATCTCCGTGCGGTTGTCAGTCTAGTTGACCTTCGTCATGAC
    CCGTCAGCAGATGATGTGCAGATGTACGAATTTCTCAAGTATTATGAGATTCCAGTCATC
    ATTGTGGCGACCAAGGCGGACAAGATTCCTCGTGGTAAATGGAACAAGCATGAATCAGCA
    ATCAAAAAGAAATTAAACTTTGACCCAAGTGACGATTTCATCCTCTTTTCATCTGTCAGC
    AAGGCAGGGATGGATGAGGCTTGGGATGCAATCTTAGAAAAATTGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    588

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNU_1482 [new locus tag: EL277_RS07800 ]
  • symbol: EngB
  • description: putative GTP-binding protein EngB
  • length: 195
  • theoretical pI: 8.40479
  • theoretical MW: 22273.3
  • GRAVY: -0.486154

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 245.2)
    and 11 more
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 59.7)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 45.3)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 41)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 40.4)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 35.6)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 35)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 30.5)
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 22)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 20.3)
    Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 17.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 12.8)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • ⊞PFAM:
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 75.7)
    and 10 more
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 29.6)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 26)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 23.5)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 16.8)
    AIG1; AIG1 family (PF04548; HMM-score: 15.6)
    HTH (CL0123) ESCRT-II; ESCRT-II complex subunit (PF05871; HMM-score: 14.1)
    P-loop_NTPase (CL0023) Septin; Septin (PF00735; HMM-score: 13.6)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 13.6)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 12.7)
    TniB; Bacterial TniB protein (PF05621; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010766
    • TAT(Tat/SPI): 0.001711
    • LIPO(Sec/SPII): 0.005011
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: BBG35365 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINTMLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYLTTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESAIKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1398 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPNU_1482&oldid=117306"
  • This page was last edited on 20 January 2022, at 17:34.
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