⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 22-FEB-2023

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_RS07930 [old locus tag: SPAP_1607 ]
pan locus tag^?: PNEUPAN002977000
symbol: SPAP_RS07930
pan gene symbol^?: cshA
• synonym:
• product: DEAD/DEAH box helicase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_RS07930 [old locus tag: SPAP_1607 ]
• symbol: SPAP_RS07930
• product: DEAD/DEAH box helicase
• replicon: chromosome
• strand: -
• coordinates: 1512140..1513714
• length: 1575
• essential: unknown

⊟Accession numbers

• Location: NC_014494 (1512140..1513714) NCBI
• BioCyc: SPAP_RS07930 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1413 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  
  GTGAAATTTAATGAATTAAACTTGTCTGCTGATTTGCTAGCAGAAATTGAAAAAGCTGGT
  TTTGTAGAAGCTAGTCCTATCCAAGAACAAACTATTCCTTTGGCCCTTGAAGGCAAGGAT
  GTTATCGGTCAAGCTCAGACTGGTACAGGAAAAACTGCAGCCTTTGGCTTGCCTACCCTT
  GAAAAAATCCGTACAGAAGAAGCGACTATCCAAGCTTTGGTCATCGCTCCAACTCGTGAA
  CTAGCTGTCCAAAGTCAAGAAGAACTCTTCCGCTTTGGTCGTAGTAAGGGAGTCAAAGTC
  CGTTCAGTATATGGCGGATCAAGCATTGAAAAACAAATTAAGGCTCTTAAATCTGGTGCC
  CATATCGTGGTGGGAACTCCAGGTCGCCTCTTGGACTTGATTAAACGCAAGGCCTTGAAA
  TTACAAGACATTGAAACCCTTATCCTTGACGAAGCGGATGAAATGCTTAACATGGGCTTC
  CTTGAAGACATCGAAGCCATTATTTCCCGTGTACCTGAGAACCGTCAAACTTTGCTTTTC
  TCAGCAACTATGCCAGATGCCATCAAACGTATCGGTGTTCAGTTTATGAAAGCCCCTGAA
  CATGTCAAGATTGCGGCTAAGGAATTGACAACAGAATTGGTTGACCAGTACTATATCCGT
  GTTAAGGAACAAGAAAAATTTGACACCATGACTCGTCTCATGGATGTGGCACAACCAGAA
  CTCGCTATTGTATTTGGTCGTACCAAACGCCGTGTGGATGAATTGACTCGTGGTTTGAAA
  ATTCGTGGCTTCCGTGCAGAAGGAATTCATGGCGACCTAGACCAAAACAAACGTCTTCGT
  GTCCTTCGTGACTTTAAAAATGGCAATCTTGATGTTTTGGTTGCGACAGACGTTGCAGCG
  CGTGGTTTGGATATTTCAGGTGTGACCCATGTCTACAACTACGATATTCCACAAGATCCT
  GAGAGTTATGTTCACCGTATCGGTCGTACAGGTCGTGCTGGTAAGTCAGGTCAATCTATT
  ACTTTTGTTGCTCCAAACGAAATGGGTTACCTTCAAATCATTGAAAACTTGACTAAGAAA
  CGCATGAAAGGTCTCAAACCTGCAAGTGTAGAAGAATCCTTCCAATCAAAAAAACAGGTA
  GCTCTCAAGAAAATCGAACGTGATTTTGCAGATGAAACCATTCGTGCCAACTTTGAGAAA
  TTTGGTAAGGATGCTCGCAAATTGGCTGCTGAGTTTACTCCAGAAGAATTGGCAATGTAT
  ATCTTGAGTCTGACAGTCCAAGACCCAGATAGCCTTCCAGAAGTGGAGATTGCACGTGAA
  AAACCACTACCGTTTAAACCATCAGGTAATGGTTTCGGTGGTAAAGCTAAGGGAGGTCGT
  GGAGGCCGTCGTGGGGACGACCGTCGAGAGCGTGATCGCCGTGGCAATGGTCGCCGTGAT
  GAGTTCAAAAAAGGAAGTCGTGGCAACGATCGTTTTGATAAGGAAAAACGTTACCGTAAG
  GATAATAAAAAACCACGCAATACTTTAAGCGAAAAGCAAACAGGCTTTGTTATTCGTAAC
  AAGGGCGATAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1575
  

⊟Protein

⊟General

• locus tag: SPAP_RS07930 [old locus tag: SPAP_1607 ]
• symbol: SPAP_RS07930
• description: DEAD/DEAH box helicase
• length: 524
• theoretical pI: 10.2388
• theoretical MW: 58827
• GRAVY: -0.597901

⊟Function

• reaction:
  EC 3.6.4.-?  ExPASy
• ⊞⊞TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 82.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 72.7)
  DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 66.8)
  and 8 more
  helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 49)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 38)
  DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 37.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 34.5)
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 28.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 20.5)
  Unknown function Enzymes of unknown specificity ATP-dependent helicase HrpB (TIGR01970; EC 3.6.4.-; HMM-score: 18.9)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 16.6)
• TheSEED: see SPAP_1607
• ⊞⊞PFAM:
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 176.7)
  and 7 more
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 110.6)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 28.3)
  CMS1; U3-containing 90S pre-ribosomal complex subunit (PF14617; HMM-score: 19.1)
  Helicase_RecD; Helicase (PF05127; HMM-score: 17.1)
  AAA_30; AAA domain (PF13604; HMM-score: 17.1)
  no clan defined DUF6363; Domain of unknown function (DUF6363) (PF19890; HMM-score: 11.8)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009073
  • TAT(Tat/SPI): 0.00156
  • LIPO(Sec/SPII): 0.000933
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000671113 NCBI
• UniProt:

⊟Protein sequence

• MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQAQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRSVYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGFLEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYYIRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQNKRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAGKSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADETIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGNGFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNTLSEKQTGFVIRNKGDK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1413 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications