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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_0772 [new locus tag: SPD_RS04135 ]
  • pan locus tag?: PNEUPAN001875000
  • symbol: SPD_0772
  • pan gene symbol?: fruB
  • synonym: pfkB
  • product: 1-phosphofructokinase, putative

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_0772 [new locus tag: SPD_RS04135 ]
  • symbol: SPD_0772
  • product: 1-phosphofructokinase, putative
  • replicon: chromosome
  • strand: +
  • coordinates: 782655..783566
  • length: 912
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP000410 (782655..783566) NCBI
  • BioCyc:
  • MicrobesOnline: 2017686 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_0772 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTTATACAGTCACACTCAATCCATCCATTGACTATATCGTTCGTTTGGACCAAGTC
    AAAGTTGGTAGTGTAAATCGTATGGACAGTGATGATAAGTTTGCTGGTGGGAAAGGAATC
    AATGTCAGCCGTGTCTTGAAACGTTTGAATATACCAAATACAGCGACGGGATTTATCGGT
    GGCTTTACTGGTAAATTTATCACAGATACTTTAGCAGAGGAAGAAATCGAGACACGTTTT
    GTCCAGGTGGCAGAAGATACTCGTATCAATGTTAAAATCAAAGCAGACCAAGAAACAGAA
    ATCAACGGAACGGGTCCAACTGTTGAACCGGTTCAGCTAGAAGAATTGAAAGCTATTTTA
    TCTAGTCTGACAGCAGAAGATACAGTTGTCTTTGCAGGTTCAAGTGCTAAAAATCTAGGC
    AATGTTATCTATAAGGATTTGATTTCCTTGACGCGCCAGACTGGTGCGCAAGTGGTCTGT
    GACTTTGAAGGACAGACCTTAATTGATAGTTTGGACTACCAGCCTCTTCTTGTAAAACCA
    AACAATCATGAACTTGGAGCGATTTTTGGGGTTAAACTCGAAAGTTTAGATGAAATTGAG
    AAATACGCTCGTGAGTTACTGGCTAAGGGTGCTCAAAATGTTATTATCTCTATGGCTGGT
    GATGGCGCCCTTCTTGTCACATCTGAGGGAGCTTACTTCGCTAAACCAATCAAAGGAACA
    GTCAAAAATTCAGTTGGAGCTGGTGATTCTATGGTTGCTGGATTCACAGGTGAATTTGTC
    AAATCAAAAGACGCAGTAGAAGCCTTCAAATGGGGAGTGGCTTGCGGAACGGCAACTACC
    TTCTCAGATGACTTGGCAACGGCGGAATTTATTAAAGAAACATATGGAAAAGTTGAGGTA
    GAAAAAAGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    912

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_0772 [new locus tag: SPD_RS04135 ]
  • symbol: SPD_0772
  • description: 1-phosphofructokinase, putative
  • length: 303
  • theoretical pI: 4.54145
  • theoretical MW: 32650.8
  • GRAVY: -0.060396

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.1.56?  ExPASy
    1-phosphofructokinase ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate
  • ⊞TIGRFAM:
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 367.3)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 361.3)
    and 6 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 156.7)
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 62.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 54)
    Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 48.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 26.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 24.9)
  • TheSEED  :
    • 1-phosphofructokinase (EC 2.7.1.56)
    Carbohydrates Monosaccharides Fructose utilization  1-phosphofructokinase (EC 2.7.1.56)
  • ⊞PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 176.2)
    and 2 more
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 30.7)
    Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021715
    • TAT(Tat/SPI): 0.000473
    • LIPO(Sec/SPII): 0.000966
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ABJ55168 NCBI
  • UniProt: A0A0H2ZPQ7 UniProt

⊟Protein sequence[edit | edit source]

  • MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSRVLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEINGTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVCDFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISMAGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACGTATTFSDDLATAEFIKETYGKVEVEKR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: lacR1 > SPD_0772 > SPD_0773 > SPD_0774

⊟Regulation[edit | edit source]

  • ⊞regulator: FruR* (repression) regulon
    FruR*(TF)important in Fructose utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0772 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPD_0772&oldid=7355"
  • This page was last edited on 20 January 2022, at 13:20.
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