⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 03-APR-2017

⊟Summary

• organism: Streptococcus pneumoniae Hu15
locus tag: SPNHU15_02273 [new locus tag: SPNHU15_RS10760 ]
pan locus tag^?: PNEUPAN003922000
symbol: SPNHU15_02273
pan gene symbol^?: comD
• synonym:
• product: ComD

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNHU15_02273 [new locus tag: SPNHU15_RS10760 ]
• symbol: SPNHU15_02273
• product: ComD
• replicon: chromosome
• strand: -
• coordinates: 2143566..2144891
• length: 1326
• essential: unknown

⊟Accession numbers

• Location: CP020551 (2143566..2144891) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_2064 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGGATTTATTTGGATTTGGGACGGTTATTGTTCATTTTTTAATTATTAGTCACAGTTAC
  CATTTTATTTGTAAAGGTCAAATAAATAGAAAAGAATTATTCGTTTTTGGTGCTTATACA
  TTACTAACAGAAATAGTATTTGATTTTCCCTTATATATTCTATATTTAGATGGGTTAGGG
  ATTGAAAGATTTTTATTTCCTTTGGGATTATATTCCTATTTTCGATGGATGAAACAGTAT
  GAGAGGGATAGAGGACTATTCCTAAGTTTACTACTATCTCTTTTATATGAGAGCACTCAT
  AACTTTCTGTCCGTAACTTTCTCCTCTATAACAGGAGATAATTTTGTTTTACAATATCAT
  TTCCCATTCTTTTTCGTTGTGACGGTGTTAACCTATTTTGTTACATTAAAAATCATTTAC
  TATTTCCATTTGGAACTAGCCTATTTTGACGAGGACTACCTTTATCCTTTCTTGAAAAAA
  GTATTTTTTGCTTTACTATTGCTACATATTGTATCTTTCGTTTCAGATATGGTAAGTACG
  ATTAAACATTTGAATAGTTTTGGAAGTATTTTGTCATCTATTGTCTTTATCTCTCTCCTT
  TTGACCTTCTTTGCAATGAATTCTCATAAAGTTCAAATGGAGAAAGAGATTGCTTTGAAG
  CAGAAGAAATTTGAACAGAAACATTTACAGAATTACACAGATGAAATTGTTGGTCTGTAT
  AATGAAATCCGTGGTTTTCGACATGATTATGCTGGAATGCTTGTCAGCATGCAGATGGCA
  ATTGACAGTGGTAATTTACAGGAAATTGACAGAATTTACAATGAAGTTTTAGTCAAAGCA
  AATCATAAATTGCGTTCAGATAAGTACACTTACTTTGATTTGAACAACATAGAAGACTCA
  GCTTTACGAAGTTTGGTTGCTCAGTCAATTGTCTATGCTCGAAATAATGGTGTAGAGTTT
  ACACTGGAAGTAAAAGATACGATTACCAAGCTTCCAATTGAACTATTGGATTTGGTTCGT
  ATCATGAGCGTTTTATTGAATAATGCTGTCGAAGGATCGGCTGATAGCTATAAAAAGCAG
  ATGGAAGTAGCAGTTATTAAGATGGAAACTGAAACAGTTATTGTGATTCAGAATTCATGT
  AAAATGACGATGACTCCTTCAGGAGATCTATTTGCCTTAGGATTCTCCACTAAGGGAAGA
  AATCGCGGAGTCGGATTAAATAATGTGAAAGAACTACTAGATAAGTACAACAATATTATT
  TTAGAAACAGAGATGGAAGGCAGTACATTTAGACAAATCATTAGATTTAAGAGGGAATTT
  GAATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1326
  

⊟Protein

⊟General

• locus tag: SPNHU15_02273 [new locus tag: SPNHU15_RS10760 ]
• symbol: SPNHU15_02273
• description: ComD
• length: 441
• theoretical pI: 6.65561
• theoretical MW: 51353.4
• GRAVY: 0.193651

⊟Function

• TIGRFAM:
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 51.8)
  and 4 more
  HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 27.7)
  no clan defined DUF6172; Family of unknown function (DUF6172) (PF19669; HMM-score: 12.6)
  DUF1514; Protein of unknown function (DUF1514) (PF07438; HMM-score: 10.7)
  DUF6524; Family of unknown function (DUF6524) (PF20134; HMM-score: 10)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009758
  • TAT(Tat/SPI): 0.000247
  • LIPO(Sec/SPII): 0.014038
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI:
• RefSeq: ARD38013 NCBI
• UniProt:

⊟Protein sequence

• MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTEIVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNFLSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for D39, TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_2064 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

O Ween, P Gaustad, L S Håvarstein
Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.
Mol Microbiol: 1999, 33(4);817-27
[PubMed:10447890] [WorldCat.org] [DOI] (P p)Bernard Martin, Chantal Granadel, Nathalie Campo, Vincent Hénard, Marc Prudhomme, Jean-Pierre Claverys
Expression and maintenance of ComD-ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae.
Mol Microbiol: 2010, 75(6);1513-28
[PubMed:20180906] [WorldCat.org] [DOI] (I p)Claudia Trappetti, Luciana Gualdi, Lorenzo Di Meola, Prashant Jain, Cindy C Korir, Paul Edmonds, Francesco Iannelli, Susanna Ricci, Gianni Pozzi, Marco R Oggioni
The impact of the competence quorum sensing system on Streptococcus pneumoniae biofilms varies depending on the experimental model.
BMC Microbiol: 2011, 11;75
[PubMed:21492426] [WorldCat.org] [DOI] (I e)M S Chandler, D A Morrison
Competence for genetic transformation in Streptococcus pneumoniae: molecular cloning of com, a competence control locus.
J Bacteriol: 1987, 169(5);2005-11
[PubMed:3032904] [WorldCat.org] [DOI] (P p)L S Håvarstein, G Coomaraswamy, D A Morrison
An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
Proc Natl Acad Sci U S A: 1995, 92(24);11140-4
[PubMed:7479953] [WorldCat.org] [DOI] (P p)E V Pestova, L S Håvarstein, D A Morrison
Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system.
Mol Microbiol: 1996, 21(4);853-62
[PubMed:8878046] [WorldCat.org] [DOI] (P p)L S Håvarstein, P Gaustad, I F Nes, D A Morrison
Identification of the streptococcal competence-pheromone receptor.
Mol Microbiol: 1996, 21(4);863-9
[PubMed:8878047] [WorldCat.org] [DOI] (P p)