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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 24-APR-2024

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_RS08690 [old locus tag: SPG_1736 ]
  • pan locus tag?: PNEUPAN003346000
  • symbol: SPG_RS08690
  • pan gene symbol?: paaI
  • synonym:
  • product: PaaI family thioesterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_RS08690 [old locus tag: SPG_1736 ]
  • symbol: SPG_RS08690
  • product: PaaI family thioesterase
  • replicon: chromosome
  • strand: -
  • coordinates: 1673528..1673932
  • length: 405
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_011072 (1673528..1673932) NCBI
  • BioCyc: SPG_RS08690 BioCyc
  • MicrobesOnline: see SPG_1736
  • PneumoBrowse for strain D39V: SPV_1632 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAAAGACTTTCATTTTGACGCTATATCTGCCTTTGAAAATTACGAAATTGAAAAAATG
    AGAGATGGTCACGTTGTGGTGACGACCAAAGTAGTGAACTCGTCGCTCAACTACTATGGC
    AATGCCCATGGTGGCTATCTATTCACGCTATGTGACCAAATCAGTGGTTTGGTGGTTATC
    TCGCTGGGACTTGATGGGGTGACACTCCAATCCTCTATCAACTACCTTAAGGCAGGAAAA
    CTCGACGATGTATTGACCATTAAAGGAGAATGTGTCCATCAAGGTCGTACAACCTGTGTG
    ATGGATGTGGATATCACCAATCAAGAGGGCAGAAATGTCTGTAAAGCAACCTTTACCATG
    TTTGTCACAGGCCAGCGGTCAGAAGAAAGACAGGTAAGGATATAA
    60
    120
    180
    240
    300
    360
    405

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_RS08690 [old locus tag: SPG_1736 ]
  • symbol: SPG_RS08690
  • description: PaaI family thioesterase
  • length: 134
  • theoretical pI: 6.08651
  • theoretical MW: 14916.9
  • GRAVY: -0.101493

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.2.-?  ExPASy
  • ⊞TIGRFAM:
    phenylacetic acid degradation protein PaaD (TIGR02286; HMM-score: 66.9)
    uncharacterized domain 1 (TIGR00369; HMM-score: 66.2)
    and 2 more
    Unknown function General acyl-CoA thioester hydrolase, YbgC/YbaW family (TIGR00051; HMM-score: 28.7)
    tol-pal system-associated acyl-CoA thioesterase (TIGR02799; EC 3.1.2.-; HMM-score: 19.4)
  • TheSEED: see SPG_1736
  • ⊞PFAM:
    HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 70.1)
    and 3 more
    DUF4442; Domain of unknown function (DUF4442) (PF14539; HMM-score: 27.7)
    4HBT_3; Thioesterase-like superfamily (PF13622; HMM-score: 26.1)
    Acyl-ACP_TE; Acyl-ACP thioesterase (PF01643; HMM-score: 17.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.076479
    • TAT(Tat/SPI): 0.000431
    • LIPO(Sec/SPII): 0.000542
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000651211 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERQVRI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SPG_RS08690 < SPG_RS08695 < SPG_RS08700

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1632 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPG_RS08690&oldid=72549"
  • This page was last edited on 20 January 2022, at 15:50.
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