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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 03-SEP-2019

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae ASP0581
  • locus tag: ASP0581_04010 [new locus tag: EL334_RS02150 ]
  • pan locus tag?: PNEUPAN001242000
  • symbol: tig
  • pan gene symbol?: tig
  • synonym:
  • product: trigger factor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: ASP0581_04010 [new locus tag: EL334_RS02150 ]
  • symbol: tig
  • product: trigger factor
  • replicon: chromosome
  • strand: +
  • coordinates: 388037..389320
  • length: 1284
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AP019192 (388037..389320) NCBI
  • BioCyc: see EL334_RS02150
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0365 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTGTATCATTTGAAAACAAAGAAACAAACCGTGGTGTCTTGACTTTCACTATCTCT
    CAAGACCAAATCAAACCAGAATTGGACCGTGTCTTCAAGTCAGTGAAGAAATCTCTTAAT
    GTTCCAGGTTTCCGTAAAGGTCACCTTCCACGCCCTATCTTCGACCAAAAATTTGGTGAA
    GAAGCTCTTTATCAAGATGCAATGAACGCACTTTTGCCAAACGCTTATGAAGCAGCTGTA
    AAAGAAGCTGGTCTTGAAGTGGTTGCCCAACCAAAAATTGACGTAACTTCAATGGAAAAA
    GGTCAAGACTGGGTTATCACTGCTGAAGTCGTTACAAAACCTGAAGTAAAATTGGGTGAC
    TACAAAAACCTTGAAGTATCAGTTGATGTAGAAAAAGAAGTAACTGACGCTGATGTCGAA
    GAGCGTATCGAACGCGAACGCAACAACCTGGCTGAATTGGTTATCAAGGAAGCTGCTGCT
    GAAAACGGCGACACTGTTGTGATCGACTTCGTTGGTTCTATCGACGGTGTTGAATTTGAC
    GGTGGAAAAGGTGAAAACTTCTCACTTGGACTTGGTTCAGGTCAATTCATCCCTGGTTTC
    GAAGACCAATTGGTAGGTCACTCAGCTGGCGAAACCGTTGATGTTATCGTAACATTCCCA
    GAAGACTACCAAGCAGAAGACCTTGCAGGTAAAGAAGCTAAATTCGTGACAACTATCCAC
    GAAGTAAAAGCTAAAGAAGTTCCAGCTCTTGACGATGAACTTGCAAAAGACATTGATGAA
    GAAGTTGAAACACTTGCTGACTTGAAAGAAAAATACCGCAAAGAATTGGCTGCTGCTAAA
    GAAGAAACTTACAAAGATACAGTTGAAGGTGCAGCAATTGATACAGCTGTAGAAAACGCT
    GAAATCGTAGAACTTCCAGAAGAAATGATCCATGAAGAAGTTCACCGTTCAGTAAATGAA
    TTCCTTGGGAACTTGCAACGTCAAGGGATCAACCCTGACATGTACTTCCAAATCACTGGA
    ACTACTCAAGAAGACCTTCACAACCAATACCAAGCAGAAGCTGAGTCACGTACTAAGACT
    AACCTTGTTATCGAAGCAGTTGCCAAAGCTGAAGGATTTGATGCTTCAGAAGAAGAAATA
    CAAAAAGAAGTTGAGCAATTGGCAGCAGACTACAACATGGAAGTTGCACAAGTTCAAAAC
    TTGCTTTCAGCTGACATGTTGAAACATGATATCACTATCAAAAAAGCTGTTGAATTGATC
    ACAAGCACAGCAACAGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1284

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: ASP0581_04010 [new locus tag: EL334_RS02150 ]
  • symbol: Tig
  • description: trigger factor
  • length: 427
  • theoretical pI: 4.15295
  • theoretical MW: 47327.4
  • GRAVY: -0.429508

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization trigger factor (TIGR00115; EC 5.2.1.8; HMM-score: 426.8)
    and 1 more
    Cellular processes Cellular processes Biosynthesis of natural products nif11-like leader peptide domain (TIGR03798; HMM-score: 8.8)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • ⊞PFAM:
    Trigger_C (CL0262) Trigger_C; Bacterial trigger factor protein (TF) C-terminus (PF05698; HMM-score: 147.7)
    no clan defined Trigger_N; Bacterial trigger factor protein (TF) (PF05697; HMM-score: 135)
    and 6 more
    FKBP (CL0487) FKBP_C; FKBP-type peptidyl-prolyl cis-trans isomerase (PF00254; HMM-score: 58.2)
    HTH (CL0123) MADF_DNA_bdg; Alcohol dehydrogenase transcription factor Myb/SANT-like (PF10545; HMM-score: 18.1)
    S15_NS1 (CL0600) WHEP-TRS; WHEP-TRS domain (PF00458; HMM-score: 13.3)
    no clan defined Focal_AT; Focal adhesion targeting region (PF03623; HMM-score: 11.3)
    Trigger_C (CL0262) SurA_N; SurA N-terminal domain (PF09312; HMM-score: 10.9)
    HTH (CL0123) PqqD; Coenzyme PQQ synthesis protein D (PqqD) (PF05402; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9981
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0015
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003942
    • TAT(Tat/SPI): 0.000517
    • LIPO(Sec/SPII): 0.001525
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: BBG80923 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEETYKDTVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNLLSADMLKHDITIKKAVELITSTATVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for TIGR4

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0365 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=ASP0581_04010&oldid=47091"
  • This page was last edited on 20 January 2022, at 14:51.
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