⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 07-FEB-2021

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_RS09400 [old locus tag: SPAP_1895 ]
pan locus tag^?: PNEUPAN003395000
symbol: SPAP_RS09400
pan gene symbol^?: msmE
• synonym:
• product: extracellular solute-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_RS09400 [old locus tag: SPAP_1895 ]
• symbol: SPAP_RS09400
• product: extracellular solute-binding protein
• replicon: chromosome
• strand: -
• coordinates: 1785644..1786903
• length: 1260
• essential: unknown

⊟Accession numbers

• Location: NC_014494 (1785644..1786903) NCBI
• BioCyc: SPAP_RS09400 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1677 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGGAATGGTATAAAAAAATCGGACTTCTTGCAACTACAGGTTTAGCTTTGGTTGGGCTC
  GGCGCTTGCTCCAACTATGGTAAATCTGCGGATGGCACAGTGACCATCGAGTATTTCAAC
  CAGAAAAAAGAAATGACCAAAACCTTGGAAGAAATCACTCGTGATTTTGAGAAGGAAAAC
  CCTAAGATCAAGGTCAAAGTCGTCAATGTACCAAATGCTGGTGAAGTATTGAAGACACGC
  GTTCTCGCAGGAGATGTGCCTGATGTGGTCAATATTTACCCACAGTCCATCGAACTGCAA
  GAATGGGCAAAAGCAGGTGTCTTTGAAGATTTGAGCAACAAAGACTACCTGAAACGCGTG
  AAAAATGGCTACGCTGAAAAATATGCTGTAAACGAAAAAGTTTACAACGTTCCTTTTACA
  GCTAATGCTTATGGAATTTACTACAACAAAGATAAATTCGAAGAACTGGGCTTGAAGGTT
  CCTGAAACCTGGGATGAATTTGAACAGTTAGTCAAAGATATCGTTGCTAAAGGACAAACA
  CCATTTGGAATTGCAGGTGCAGATGCTTGGACACTCAATGGTTACAATCAATTAGCCTTT
  GCGACAGCAACAGGTGGAGGAAAAGAAGCAAATCAATACCTTCGTTATTCTCAACCAAAT
  GCCATTAAATTGTCGGATCCGATTATGAAAGATGATATCAAGGTCATGGACATCCTTCGC
  ATCAATGGCTCTAAGCAAAAGAACTGGGAAGGTGCTGGCTATACCGATGTTATCGGAGCC
  TTCGCACGTGGGGATGTCCTCATGACACCAAATGGGTCTTGGGCGATCACAGCGATTAAT
  GAACAAAAACCGAACTTTAAGATTGGGACCTTCATGATTCCAGGAAAAGAAAAAGGACAA
  AGCTTAACCGTTGGTGCGGGAGACTTGGCATGGTCTATCTCAGCCACCACCAAACATCCA
  AAAGAAGCCAATGCCTTTGTGGAATATATGACCCGTCCAGAAGTCATGCAAAAATACTAC
  GATGTGGACGGATCTCCAACAGCGATCGAAGGGGTCAAACAAGCAGGAGAAGATTCACCG
  CTTGCTGGTATGACCGAATATGCCTTTACGGATCGTCACTTGGTCTGGTTGCAACAATAC
  TGGACCAGTGAAGCAGACTTCCATACCTTGACCATGAACTATGTCTTGACCGGTGATAAA
  CAAGGCATGGTCAATGATTTGAATGCCTTCTTTAACCCGATGAAAGCGGATGTGGATTAG
  60
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  360
  420
  480
  540
  600
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  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  

⊟Protein

⊟General

• locus tag: SPAP_RS09400 [old locus tag: SPAP_1895 ]
• symbol: SPAP_RS09400
• description: extracellular solute-binding protein
• length: 419
• theoretical pI: 4.92369
• theoretical MW: 46612.5
• GRAVY: -0.410979

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter, N-acetylglucosamine/diacetylchitobiose-binding protein (TIGR03851; HMM-score: 83.4)
  and 1 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 65.7)
• TheSEED: see SPAP_1895
• ⊞⊞PFAM:
  PBP (CL0177) SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 146)
  SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 145.4)
  and 3 more
  SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 34.3)
  SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 21.9)
  no clan defined ACP_PD; Acyl carrier protein phosphodiesterase (PF04336; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞⊞SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
  • SP(Sec/SPI): 0.003397
  • TAT(Tat/SPI): 0.000181
  • LIPO(Sec/SPII): 0.995935
  • Cleavage Site: CS pos: 22-23. LGA-CS. Pr: 0.9965
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000455429 NCBI
• UniProt:

⊟Protein sequence

• MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKEMTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKVPETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAITAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPEVMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTMNYVLTGDKQGMVNDLNAFFNPMKADVD

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1677 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications