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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr0046 [new locus tag: SPR_RS00245 ]
  • pan locus tag?: PNEUPAN000690000
  • symbol: purL
  • pan gene symbol?: purL
  • synonym:
  • product: Phosphoribosylformylglycinamide synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr0046 [new locus tag: SPR_RS00245 ]
  • symbol: purL
  • product: Phosphoribosylformylglycinamide synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 46493..50221
  • length: 3729
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    TTGATGGATAAACGTATTTTTGTTGAAAAAAAGGCTGATTTTCAGGTCAAGTCAGAGAGT
    TTGGTTAGAGAGCTCCAGCACAACTTGGGACTGTCAAGCTTGAAAAGTATTCGTATTGTG
    CAAGTATATGATGTATTTGACTTGGCTGAGGACTTGTTTGCACCTGCAGAGAAGCACATT
    TTCTCTGAGCAGGGAACCGACCATGTTTTAGATGAAGTATCTGTGCAGGCGGATCTTGCT
    AACTATGCTTTCTTTGCCATTGAAAGTCTGCCAGGGCAGTTTGACCAGCGTGCAGCTTCG
    TCACAGGAAGCCTTGCTTTTGTTGGGAAGTTCGAGTGACGTGACAGTTAACACAGCCCAA
    CTTTACTTGGTGAATAAAGATATTGATGCGACTGAGTTGGAAGCTGTCAAAAACTACCTG
    CTCAATCCAGTTGATTCTCGTTTCAAGGATATCACGACAGGGATTGCCAAGCAGGAGTTT
    TCAGAGTCAGACAAGACCATTCCAAAATTGACTTTCTTTGAAAGCTATACAGCAGAAGAC
    TTTGCTCGCTACAAGGCCGAGCAAGGGATGGCCATGGAAGTGGATGATTTGCTCTTTATC
    CAAGACTACTTTAAGTCAATCGGGCGCGTGCCAACTGAGACTGAACTCAAGGTTTTGGAC
    ACTTACTGGTCTGACCACTGCCGTCATACGACTTTTGAGACAGAGTTGAAACACATCGAC
    TTTTCAGCTTCTAAATTTCAAAAGCAATTGCAGTCAACCTATGACAAGTATATTGCCATG
    CGCGAGGAGTTAGGTCGGTCTGAAAAACCACAAACCTTGATGGATATGGCGACTATTTTC
    GGTCGTTATGAGCGTGCTAACGGACGATTGGATGATATGGAAGTCTCTGACGAAATCAAT
    GCCTGCTCAGTTGAAATTGAAGTGGATGTTGATGGTGTCAAGGAACCTTGGCTCCTCATG
    TTTAAAAATGAAACCCACAACCATCCAACAGAAATTGAGCCATTTGGTGGAGCGGCTACC
    TGTATCGGTGGAGCTATTCGTGATCCGTTGTCAGGCCGTTCCTATGTTTACCAAGCCATG
    CGTATTTCAGGTGCTGGTGATATTACAGCACCGATTTCGGAAACTCGTGCTGGGAAATTG
    CCACAACAAGTCATTTCTAAAACAGCAGCTCATGGTTATTCTTCATATGGTAACCAGATT
    GGGCTTGCCACAACCTACGTTCGTGAATACTTCCACCCAGGCTTTGTAGCTAAACGTATG
    GAACTTGGTGCCGTTGTTGGTGCGGCTCCCAAGGGCAATGTTGTCCGTGAAAAACCTGAA
    GCAGGTGATGTGATCATCCTTCTCGGAGGTAAAACAGGTCGTGATGGTGTCGGTGGTGCG
    ACGGGCTCTTCTAAGGTTCAAACAGTTGAGTCTGTAGAGACTGCTGGTGCTGAGGTTCAA
    AAAGGAAATGCCATCGAAGAACGCAAGATTCAGCGCCTCTTCCGTAATGGCAATGTCACT
    CGTCTGATCAAGAAGTCCAATGACTTTGGGGCAGGCGGTGTCTGTGTGGCTATCGGTGAA
    TTGGCAGACGGTCTTGAAATCGACCTCAACAAGGTGCCTCTTAAATACCAGGGCTTGAAT
    GGTACAGAAATTGCCATCTCTGAATCACAAGAACGGATGGCGGTTGTGGTTCGTCCTGAA
    GATGTGGATACCTTCGTTGCCGAATGTAACAAAGAAAATATTGATGCTGTTGTGGTGGCG
    ACAGTAACTGAAAAACCAAATCTTGTCATGCACTGGAATGGTGAGACAATCGTTGACTTG
    GAGCGTCGTTTCCTTGACATCAATGGTGTGCGCGTGGTTGTCGATGCCAAAGTTGTGGAC
    AAGGATGTCAAACTCCCAGAAGAGCGTCAAACATCTGCTGAAACACTGGAATCAGATACC
    CTTACGGTTCTATCTGACCTCAACCATGCAAGTCAAAAAGGATTACAGACTATCTTTGAC
    TGCTCTGTTGGACGCTCAACGGTTAATCACCCACTTGGTGGTCGTTACCAACTCACACCA
    ACTGAGGCATCTGTGCAGAAATTGCCAGTTCAACACGGTGTGACTCATACTGCGTCGGTC
    ATTGCTCAAGGTTTCAACCCATATGTAGCTGAATGGTCTCCATACCACGGTGCTGCTTAT
    GCGGTTATCGAAGCAACTGCTCGTTTGGTGGCTGCTGGTGCCAACTGGTCCAAGGCTCGT
    TTCTCTTACCAAGAATATTTCGAGCGTATGGACAAGCAAGCAGAGCGTTTTGGTCAGCCA
    GTAGCCGCTCTTCTAGGCTCTATTGAAGCTCAGATTCAACTTGGCTTGCCATCTATCGGT
    GGTAAGGACTCCATGTCTGGTACCTTTGAAGAATTGACCGTTCCGCCAACCTTGGTTGCC
    TTTGGGGTGACGACGGCAGATAGCCGTAAGGTGCTCTCTCCAGAATTTAAAGCTGTTGGG
    GAAAATATCTACTACATCCCAGGTCAAGCCCTCTCTGCAGAGATTGATTTTGACTTGATT
    AAGAAAAATTTTGCTCAGTTTGAAGCAAGCCAAGCTGACCATAAAGTGACATCTGCATCA
    GCTGTCAAATACGGTGGTGTAGTTGAAAGTTTGGCTCTTGCTACCTTTGGAAACTATATT
    GGTGCAGAGGTGACCTTGCCTGAACTTAAAACAGCTTTGACAGCTCAATTAGGCGGCTTT
    GTCTTCACATCTCCTGAAGAAATTGCTGGAGTAGAGAAGATTGGACAAACGAAAGCAGAC
    TTTACACTGACTGTCAACGGTGTGAAGCTAGATGGACACAAGCTTGACAGTGCATTTCAA
    GGGACATTGGAAGAAGTTTACCCAACAGAATTTACCCAAGCGAAAGAACTAGAAGAAGTA
    CCAGCTGTGGCATCAGATGTTGTGATTAAAGCCAAAGAAAAGGTTGAAAAACCTGTGGTT
    TACATCCCAGTCTTTCCAGGAACCAACTCAGAATATGATTCAGCTAAGGCCTTCGAAAAA
    GAAGGTGCAGAGGTCAATTTGGTGCCATTCGTGACCTTGAATGAAGAAGCTATTGTCAAG
    TCAGTTGAAACTATGGTTGACAATATCGACAAGACTAATATTCTCTTCTTTGCTGGTGGA
    TTCTCGGCTGCGGATGAACCAGATGGTTCAGCTAAGTTTATCGTCAATATCCTGCTTAAT
    GAAAAAGTGCGTGTGGCTATTGATAGCTTTATCGCCCGTGGTGGTTTGATTATCGGTATT
    TGTAATGGATTCCAAGCCTTAGTCAAATCGGGTCTCCTACCCTACGGAAACTTTGAAGAT
    GCTAACAATACTAGCCCAACCCTCTTCTACAATGATGCCAACCAACACGTGGCCAAGATG
    GTGGAAACTCGCATTGCCAATACCAACTCACCATGGTTGGCTGGTGTGCAAGTGGGCGAT
    ATCCACGCTATTCCTGTTTCGCACGGTGAAGGGAAGTTTGTCGTGACGGCTGAGGAATTT
    GCAGAGCTCCGTGACAATGGACAAATTTTCAGCCAATACGTTGACTTTAACGGTAAACCA
    AGTATGGATTCTAAGTACAATCCGAATGGTTCTGTCCATGCCATCGAAGGAATTACCAGC
    AAGAATGGTCAAATCATCGGTAAGATGGGCCATTCAGAACGTTATGAGGACGGTCTTTTC
    CAAAATATCCCAGGCAATAAAGACCAGCACCTGTTCGCGTCAGCGGTTAAACATTTCACT
    GGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3729

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr0046 [new locus tag: SPR_RS00245 ]
  • symbol: PurL
  • description: Phosphoribosylformylglycinamide synthetase
  • length: 1242
  • theoretical pI: 4.71859
  • theoretical MW: 135703
  • GRAVY: -0.214815

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.5.3?  ExPASy
    Phosphoribosylformylglycinamidine synthase ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 2207.4)
    and 5 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 276.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 232.7)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 146.8)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 14.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 12.7)
  • TheSEED  :
    • Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
    • Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
    and 1 more
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)
  • PFAM:
    Glutaminase_I (CL0014) GATase_5; CobB/CobQ-like glutamine amidotransferase domain (PF13507; HMM-score: 258.5)
    and 4 more
    no clan defined AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 85.1)
    FGAR-AT_linker; Formylglycinamide ribonucleotide amidotransferase linker domain (PF18072; HMM-score: 46.4)
    Glutaminase_I (CL0014) GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 18.3)
    DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001204
    • TAT(Tat/SPI): 0.000218
    • LIPO(Sec/SPII): 0.000196
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDVFDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQEALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFSESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVLDTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMATIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPFGGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGYSSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGKTGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDFGAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVAECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKLPEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEASVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARFSYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLVAFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVTSASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIGQTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKEKVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDKTNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVKSGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVSHGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQIIGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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