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serotype 4
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D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr0122 [new locus tag: SPR_RS00670 ]
  • pan locus tag?: PNEUPAN000794000
  • symbol: trmU
  • pan gene symbol?: mnmA
  • synonym:
  • product: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr0122 [new locus tag: SPR_RS00670 ]
  • symbol: trmU
  • product: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 130562..131737
  • length: 1176
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGAAGCACACACGACCTTCAATCGTGAATAAACGAATAGATGGGAGACTTACCATGAGT
    GATAACTCTAAAACACGTGTTGTCGTGGGGATGAGTGGTGGTGTTGATTCGTCGGTGACG
    GCTCTTTTGCTCAAGGAGCAGGGCTACGATGTGATCGGTATCTTCATGAAGAACTGGGAT
    GACACAGATGAAAACGGCGTCTGTACGGCGACCGAAGATTACAAGGATGTGGTTGCGGTG
    GCAGATCAGATTGGCATTCCCTACTACTCTGTCAATTTTGAAAAAGAGTACTGGGACCGC
    GTTTTTGAGTATTTCCTAGCTGAATACCGTGCAGGGCGCACGCCAAATCCGGACGTTATG
    TGCAACAAGGAAATCAAGTTCAAGGCCTTTTTGGACTATGCCATGACCTTGGGGGCAGAC
    TATGTAGCGACTGGGCACTATGCTCGAGTGGCGCGTGATGAGGATGGCACTGTTCACATG
    CTTCGTGGCGTGGACAATGGCAAGGATCAGACCTATTTCCTCAGCCAACTTTCGCAAGAA
    CAACTTCAAAAAACCATGTTCCCACTAGGACATTTGAAAAAGCCTGAAGTTCGAAAACTA
    GCAGAAGAAGCAGGTCTTTCGACTGCTAAGAAGAAAGACTCGACAGGGATTTGCTTTATC
    GGAGAAAAGAACTTTAAAAACTTTCTCAGCAACTACCTGCCAGCTCAGCCTGGTCGTATG
    ATGACTGTGGATGGTCGTGATATGGGCGAGCATGCTGGTCTTATGTACTATACAATCGGT
    CAGCGTGGCGGACTCGGTATCGGTGGGCAACACGGTGGTGACAATGCCCCTTGGTTCGTT
    GTCGGAAAAGATCTAAGCAAGAATATTCTCTATGTAGGCCAAGGTTTCTACCATGATTCG
    CTCATGTCAACCAGCCTAGAGGCTAGCCAAGTCCACTTTACTCGTGAAATGCCAGAAGAG
    TTTACGCTAGAATGTACGGCTAAATTCCGTTACCGTCAGCCTGACTCTAAGGTGACCGTC
    CATGTCAAAGGAGACAAGGCAGAAGTTATCTTTGCGGAACCACAACGCGCGATTACACCA
    GGACAGGCAGTTGTCTTTTACGATGGCGAAGAGTGTCTAGGTGGCGGTTTGATTGACAAT
    GCTTACCGCGATGGACAAGTTTGTCAGTACATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr0122 [new locus tag: SPR_RS00670 ]
  • symbol: TrmU
  • description: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
  • length: 391
  • theoretical pI: 5.86731
  • theoretical MW: 43731.2
  • GRAVY: -0.4289

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.61?  ExPASy
    tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 457.3)
    and 11 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 29.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 23.5)
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 20.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 19.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 18.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 18.2)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 17)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 15.9)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 13.5)
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 11.4)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 10.9)
  • TheSEED  :
    • tRNA-uridine(34) 2-sulfurtransferase MnmA (EC 2.8.1.13)
  • PFAM:
    HUP (CL0039) tRNA_Me_trans; tRNA methyl transferase HUP domain (PF03054; HMM-score: 295.5)
    and 7 more
    no clan defined tRNA_Me_trans_C; Aminomethyltransferase beta-barrel domain (PF20258; HMM-score: 84.1)
    PRC-barrel (CL0350) tRNA_Me_trans_M; tRNA methyl transferase PRC-barrel domain (PF20259; HMM-score: 78.3)
    HUP (CL0039) NAD_synthase; NAD synthase (PF02540; HMM-score: 30.2)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 19.6)
    Asn_synthase; Asparagine synthase (PF00733; HMM-score: 16.8)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 16.8)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 15.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9837
    • Cytoplasmic Membrane Score: 0.002
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0144
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012693
    • TAT(Tat/SPI): 0.000868
    • LIPO(Sec/SPII): 0.001689
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKHTRPSIVNKRIDGRLTMSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTDENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLSQEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQPGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQGFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFAEPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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