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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr0232 [new locus tag: SPR_RS01215 ]
  • pan locus tag?: PNEUPAN000984000
  • symbol: pflF
  • pan gene symbol?: pfl
  • synonym:
  • product: Formate acetyltransferase 3

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr0232 [new locus tag: SPR_RS01215 ]
  • symbol: pflF
  • product: Formate acetyltransferase 3
  • replicon: chromosome
  • strand: +
  • coordinates: 222574..225021
  • length: 2448
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    GTGCTTACGATGGTAAATACAGAAGTAGCAAGAACAACAATCAAGACAGAATATTTTGGC
    AGCCTTACTGAAAGGATGAACAAATATCGAGAAGATGTTTTAAATAAAAAACCTTATATT
    GATGCTGAGAGAGCAGTTCTAGCAACACGCGCCTATGAACGATACAAGGAACAACCTAAT
    GTCCTAAAACGTGCATATATGCTGAAAGAAATTTTGGAAAATATGTCTATCTATATTGAA
    GAAGAATCTATGATTGCGGGAAATCAAGCTTCTTCCAATAAAGATGCTCCTATTTTTCCG
    GAATATACGCTAGAATTTGTTCTCAATGAGTTGGATCTTTTTGAAAAGCGTGATGGAGAT
    GTTTTCTATATTACAGAAGAAACAAAAGAACAACTTAGAAGTATTGCTCCGTTTTGGGAA
    AATAATAATTTACGTGCTAGAGCTGGTGCCTTATTACCTGAAGAAGTGTCTGTTTATATG
    GAAACAGGATTCTTCGGTATGGAAGGTAAGATGAATTCTGGAGATGCTCACTTAGCAGTT
    AACTATCAGAAACTTTTGCAATTTGGTTTAAGAGGTTTTGAAGAGCGGGCTCGTAAAGCA
    AAAGTAGCTCTAGATTTAACAGATCCAGCAAGTATTGATAAATATCATTTTTACGACTCT
    ATATTTATCGTAATCGATGCTATTAAAGTATATGCAAAGCGCTTTGTTGCTCTTGCTAAA
    AGTTTAGCCGAAAATGCAAATCCTAAACGTAAGAAAGAATTACTTGAGATTGCAGATATT
    TGCTCTAGAGTCCCATATGAACCGGCAACTACTTTTGCAGAAGCTATTCAATCAGTTTGG
    TTTATTCAATGTATTTTACAAATTGAATCTAATGGCCACTCTCTTTCATATGGCCGTTTT
    GATCAATATATGTATCCATATATGAAGGCTGATTTAGAAAGTGGTAAAGAAACAGAAGAT
    AGCATTGTTGAACGTCTGACAAATCTTTGGATTAAGACAATTACAATCAATAAGGTTCGC
    AGTCAATCACATACATTTTCTTCAGCAGGAAGTCCTTTATATCAAAATGTTACAATTGGT
    GGACAGACTCGAGATAAGAAGGATGCTGTTAACCCATTATCTTATTTGGTATTAAAATCA
    GTTGCACAAACCCATCTACCGCAACCTAATCTAACTGTACGTTACCATGCAGGTTTAGAT
    GCTCGTTTCATGAATGAGTGTATTGAAGTGATGAAACTTGGTTTTGGTATGCCTGCATTT
    AATAATGATGAGATTATTATTCCTTCTTTTATTGCAAAAGGAGTATTGGAAGATGATGCT
    TATGATTACAGTGCCATTGGATGTGTTGAAACGGCAGTTCCAGGGAAATGGGGCTATCGT
    TGCACAGGTATGAGTTATATGAACTTCCCTAAGGTTCTACTTATCACGATGAATGATGGA
    ATTGATCCGGCTTCGGGTAAACGGTTTGCACCAAGCTTTGGTCATTTTAAGGATATGAAG
    AACTTTTCTGAATTAGAAAATGCTTGGGATAAAACACTAAGATATTTGACACGAATGAGT
    GTTATTGTTGAAAATTCTATTGATTTATCATTGGAACGAGAAGTTCCTGATATTCTATGT
    TCAGCATTGACTGATGATTGTATTGGTCGTGGAAAACACCTTAAAGAAGGTGGAGCAGTA
    TATGATTATATATCAGGATTGCAAGTTGGAATTGCAAATTTGTCGGATTCATTAGCTGCA
    ATTAAAAAATTGGTGTTTGAGGAAGAACGTATAAGCCCAAGTCAGCTTTGGCATGCACTG
    GAAACAGATTATGCCGGAGAAGAAGGTAAGGTCATTCAAGAAATGTTGATTCATGATGCA
    CCTAAGTATGGTAATGATGATGATTATGCTGACAAATTGGTTACTGCTGCTTATGACATT
    TATGTTGATGAAATTGCTAAATATCCTAATACACGTTATGGAAGAGGGCCTATTGGAGGA
    ATTCGTTATTCAGGAACATCTTCTATATCAGCCAACGTAGGGCAGGGACGTGGAACATTA
    GCAACTCCAGATGGACGCAACGCGGGTACACCGTTAGCAGAGGGTTGTTCACCATCACAT
    AATATGGATCAACACGGCCCTACATCTGTTTTAAAATCTGTTTCAAAATTACCAACAGAT
    GAAATCGTAGGTGGGGTTCTCTTAAATCAGAAAGTAAATCCTCAAACGTTAGCCAAAGAA
    GAAGATAAATTAAAACTAATTGCTTTGTTACGAACATTCTTTAATCGTTTACATGGGTAC
    CATATTCAATACAATGTTGTTTCCAGAGAGACGTTGATTGACGCTCAGAAACATCCTGAA
    AAACACAGAGACTTAATTGTTCGTGTTGCAGGATACTCTGCATTCTTCAATGTTCTTTCT
    AAGGCAACCCAAGATGACATTATAGGACGTACTGAGCATACTTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2448

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr0232 [new locus tag: SPR_RS01215 ]
  • symbol: PflF
  • description: Formate acetyltransferase 3
  • length: 815
  • theoretical pI: 5.69489
  • theoretical MW: 91684.6
  • GRAVY: -0.333374

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.54?  ExPASy
    Formate C-acetyltransferase Acetyl-CoA + formate = CoA + pyruvate
  • TIGRFAM:
    glycyl radical enzyme, PFL2/glycerol dehydratase family (TIGR01774; HMM-score: 909.8)
    and 3 more
    Metabolism Energy metabolism Amino acids and amines choline trimethylamine-lyase (TIGR04394; EC 4.3.99.4; HMM-score: 530.8)
    Metabolism Energy metabolism Fermentation formate acetyltransferase (TIGR01255; EC 2.3.1.54; HMM-score: 186.5)
    autonomous glycyl radical cofactor GrcA (TIGR04365; HMM-score: 45.6)
  • TheSEED  :
    • Glycerol dehydratase, B12-independent (EC 4.2.1.30)
    Carbohydrates Fermentation Fermentations: Mixed acid  Pyruvate formate-lyase (EC 2.3.1.54)
  • PFAM:
    PFL-like (CL0339) PFL-like; Pyruvate formate lyase-like (PF02901; HMM-score: 681.6)
    and 1 more
    Gly_radical; Glycine radical (PF01228; HMM-score: 111.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8514
    • Cytoplasmic Membrane Score: 0.002
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.1463
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00344
    • TAT(Tat/SPI): 0.000299
    • LIPO(Sec/SPII): 0.001354
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLTMVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETGFFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSIFIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITINKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQVGIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDDDYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDGRNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIGRTEHTL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: DeoR/PflR (repression) regulon
    DeoR/PflR(TF)important in Formate metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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