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serotype 4
D39
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D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
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A66
serotype 3
AP200
serotype 11A
ASP0581
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ATCC 49619
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ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr0286 [new locus tag: SPR_RS01490 ]
  • pan locus tag?: PNEUPAN001105000
  • symbol: hysA
  • pan gene symbol?: spnHL
  • synonym:
  • product: Hyaluronate lyase precursor (hyaluronidase/hyase)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr0286 [new locus tag: SPR_RS01490 ]
  • symbol: hysA
  • product: Hyaluronate lyase precursor (hyaluronidase/hyase)
  • replicon: chromosome
  • strand: +
  • coordinates: 285103..288339
  • length: 3237
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    TTGATTTTACAATATGTTTATTGGAGTGTATACATGCAAACAAAAACAAAGAAGCTCATT
    GTGAGTTTGTCTTCACTTGTTTTATCAGGATTTTTATTAAACCATTATATGACAGTTGGA
    GCGGAAGAAACGACTACGAATACCATTCAGCAAAGCCAGAAGGAAGTTCAGTATCAGCAA
    AGGGATACAAAAAATTTAGTTGAAAATGGTGATTTTGGTCAGACGGAGGACGGAAGCAGT
    CCGTGGACAGGAAGCAAAGCTCAGGGGTGGTCAGCTTGGGTAGACCAGAAGAATAGTTCT
    GCAGATGCCTCAACTCGAGTCATTGAGGCTAAGGATGGGGCTATCACTATCTCAAGCCCT
    GAGAAATTAAGGGCAGCGGTTCACCGTATGGTTCCTATTGAAGCTAAGAAAAAGTATAAA
    CTGCGTTTCAAGATTAAAACAGATAATAAAGTCGGGATTGCCAAAGTTCGTATCATTGAG
    GAAAGTGGTAAGGACAAGCGATTGTGGAATTCTGCAACGACGTCAGGAACAAAGGACTGG
    CAGACCATTGAAGCAGACTATAGCCCGACTTTAGATGTTGATAAAATCAAGCTGGAGTTA
    TTCTATGAAACAGGAACTGGGACTGTTTCCTTTAAGGATATTGAGCTGGTAGAGGTAGCA
    GACCAGCCTTCTGAGGATTCTCAAACAGATAAACAACTTGAGGAAAAGATTGATTTACCA
    ATTGGAAAAAAACATGTTTTTTCTCTTGCGGACTATACTTATAAGGTAGAAAATCCTGAC
    GTTGCTTCAGTCAAAAATGGAATTTTAGAACCTCTTAAGGAAGGGACAACCAATGTCATT
    GTCAGTAAAGATGGCAAGGAAGTGAAAAAGATTCCTTTGAAGATTCTAGCCTCTGTTAAG
    GATACATACACAGACCGTTTGGATGACTGGAATGGCATCATCGCTGGGAATCAATACTAT
    GATTCTAAAAATGAACAGATGGCCAAATTAAACCAGGAATTGGAAGGAAAGGTAGCTGAT
    AGCCTATCCAGTATTTCAAGTCAGGCGGACCGCATCTATTTGTGGGAAAAATTTTCAAAT
    TATAAAACGTCTGCAAATCTGACTGCCACTTATCGGAAATTGGAGGAGATGGCCAAGCAA
    GTGACCAATCCTTCTTCTCGTTATTATCAAGATGAAACTGTCGTTCGAACAGTCAGGGAT
    TCCATGGAATGGATGCATAAACATGTCTACAATAGTGAAAAGAGCATTGTTGGGAACTGG
    TGGGATTATGAAATCGGTACACCTCGTGCCATCAACAATACCTTGTCTCTGATGAAAGAA
    TACTTCTCTGATGAGGAAATTAAAAAATATACAGATGTGATTGAAAAATTTGTACCAGAT
    CCCGAACATTTCCGAAAGACGACTGATAACCCATTCAAGGCTCTAGGTGGAAACTTAGTT
    GATATGGGAAGGGTAAAAGTAATAGCTGGTTTACTGCGTAAGGATGATCAAGAAATTTCT
    TCTACCATTCGCTCGATTGAGCAAGTGTTCAAGTTGGTAGACCAAGGTGAAGGTTTTTAT
    CAAGATGGATCCTATATCGACCACACCAATGTTGCCTATACGGGTGCTTATGGGAATGTT
    TTGATTGATGGCCTGTCTCAACTGTTGCCAGTCATTCAAAAGACCAAGAATCCAATCGAT
    AAAGATAAAATGCAAACCATGTACCACTGGATTGATAAATCGTTTGCTCCTTTGCTGGTG
    AATGGAGAGCTGATGGATATGAGTCGTGGACGCTCGATCAGTCGTGCAAATAGCGAGGGG
    CACGTGGCCGCAGTAGAAGTACTAAGAGGGATTCACCGAATAGCGGATATGTCTGAAGGA
    GAAACCAAACAACGTTTGCAGAGTCTTGTGAAGACCATTGTTCAATCGGATAGTTATTAT
    GATGTCTTTAAGAATTTGAAGACTTATAAGGATATCAGTTTGATGCAATCCTTGTTAAGT
    GATGCAGGAGTCGCAAGTGTTCCAAGAACAAGTTACCTATCTGCCTTTAACAAGATGGAT
    AAAACAGCCATGTACAATGCAGAGAAAGGGTTTGGATTTGGCTTGTCACTCTTTTCCAGT
    CGTACCTTGAATTACGAACACATGAACAAGGAAAATAAACGTGGTTGGTATACGAGTGAT
    GGGATGTTCTATCTTTACAATGGCGATTTGAGTCACTATAGCGATGGCTACTGGCCAACA
    GTTAATCCATATAAGATGCCTGGTACAACAGAGACGGATGCTAAGAGAGCGGATAGCGAT
    ACAGGTAAAGTTTTACCGTCTGCTTTCGTTGGAACGAGCAAACTAGATGATGCCAATGCG
    ACAGCAACCATGGATTTCACCAACTGGAATCAAACATTGACTGCTCATAAGAGCTGGTTT
    ATGCTAAAGGATAAGATTGCCTTTTTAGGAAGCAATATCCAAAACACTTCAACAGATACT
    GCTGCAACTACAATTGACCAGAGAAAACTGGAATCAAGTAATCCATATAAAGTCTATGTC
    AATGATAAAGAAGCCTCCCTTACAGAACAAGAAAAGGATTATCCTGAAACCCAAAGTGTC
    TTTTTAGAATCGTCCGATTCGAAAAAGAATATTGGTTACTTTTTCTTTAAGAAGAGTTCA
    ATCAGTATGAGTAAGGCTTTGCAAAAGGGAGCCTGGAAGGATATCAATGAAGGACAGTCA
    GACAAGGAAGTTGAAAATGAATTTCTTACGATTAGTCAGGCTCATAAGCAAAATGGAGAT
    TCTTATGGCTATATGCTCATTCCTAACGTGGATCGTGCCACCTTCAATCAAATGATAAAA
    GAGTTAGAAAGTAGCCTCATCGAAAATAACGAAACCCTTCAGTCTGTTTATGATGCCAAA
    CAAGGAGTTTGGGGCATTGTGAAATATGATGATTCTGTCTCTACTATTTCCAACCAATTC
    CAAGTTTTGAAACGTGGAGTCTATACTATTCGAAAAGAAGGGGATGAATATAAGATTGCC
    TACTATAATCCTGAAACCCAGGAATCAGCTCCAGATCAGGAAGTCTTTAAAAAGCTAGAG
    CAAGCAGCTCAGCCACAAGTACAGAATTCAAAAGAAAAGGAAAAATCTGAAGAGGAAAAG
    AACCATTCGGATCAAAAGAATCTCCCTCAGACAGGAGAAGGTCAGTCAATCTTGGCAAGT
    CTAGGGTTCTTGCTACTTGGGGCGTTTTATTTATTCCGTAGAGGAAAGAACAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3237

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr0286 [new locus tag: SPR_RS01490 ]
  • symbol: HysA
  • description: Hyaluronate lyase precursor (hyaluronidase/hyase)
  • length: 1078
  • theoretical pI: 5.89087
  • theoretical MW: 122176
  • GRAVY: -0.662338

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.2.1?  ExPASy
    Hyaluronate lyase Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
  • TIGRFAM:
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 26.5)
  • TheSEED  :
    • Hyaluronate lyase (EC 4.2.2.1)
    Virulence, Disease and Defense Virulence, Disease and Defense - no subcategory Streptococcus pyogenes Virulome  Hyaluronate lyase precursor (EC 4.2.2.1)
  • PFAM:
    Hy-ly_N (CL0372) Lyase_8_N; Polysaccharide lyase family 8, N terminal alpha-helical domain (PF08124; HMM-score: 434.4)
    and 5 more
    Gal_mutarotase (CL0103) Lyase_8; Polysaccharide lyase family 8, super-sandwich domain (PF02278; HMM-score: 229.9)
    no clan defined Lyase_8_C; Polysaccharide lyase family 8, C-terminal beta-sandwich domain (PF02884; HMM-score: 46.3)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 18.4)
    NusG_II; NusG domain II (PF07009; HMM-score: 10.6)
    DUF4449; Protein of unknown function (DUF4449) (PF14613; HMM-score: 9.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 9.49
    • Extracellular Score: 0.5
    • Internal Helices: 2
  • SignalP: Signal peptide SP(Sec/SPI) length 41 aa
    • SP(Sec/SPI): 0.844786
    • TAT(Tat/SPI): 0.003071
    • LIPO(Sec/SPII): 0.004643
    • Cleavage Site: CS pos: 41-42. VGA-EE. Pr: 0.7650
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MILQYVYWSVYMQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKEVQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDGAITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNSATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQTDKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEVKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSISSQADRIYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEWMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPEHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFYQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPLLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQSDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFGLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTETDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLGSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSKKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYMLIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLKRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNHSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, RegR (repression) regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 
    RegR(TF)important in Hyaluronate utilization; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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