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PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 30-JAN-2014
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae R6
- locus tag: spr0454 [new locus tag: SPR_RS02325 ]
- pan locus tag?: PNEUPAN001403000
- symbol: grpE
- pan gene symbol?: grpE
- synonym:
- product: Heat-shock protein (activation of DnaK)
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: spr0454 [new locus tag: SPR_RS02325 ]
- symbol: grpE
- product: Heat-shock protein (activation of DnaK)
- replicon: chromosome
- strand: +
- coordinates: 461030..461578
- length: 549
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: AE007317 (461030..461578) NCBI
- BioCyc: SPR0454 BioCyc
- MicrobesOnline: 133339 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_0459 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541TTGAAATCATTAAAGGAGGCGAACATGGCCCAAGATATAAAAAATGAAGAAGTAGAAGAA
GTTCAAGAAGAGGAAGTTGTGGAAACAGCTGAAGAAACAACTCCTGAAAAGTCTGAGTTG
GACTTGGCAAATGAACGTGCAGATGAGTTCGAAAACAAATATCTTCGCGCTCATGCAGAA
ATGCAAAATATCCAACGCCGTGCCAATGAAGAACGTCAAAACTTGCAACGTTATCGTAGC
CAGGACTTGGCAAAAGCAATCTTACCATCTCTTGACAACCTTGAGCGTGCACTTGCAGTT
GAAGGTTTGACAGATGATGTGAAGAAGGGCTTGGCGATGGTGCAAGAAAGCTTGATTCAC
GCTTTGAAAGAAGAAGGAATTGAAGAAATCGCAGCAGATGGCGAATTTGACCATAACTAC
CATATGGCCATCCAAACTCTCCCAGGAGACGATGAACACCCAGTAGATACCATCGCCCAA
GTCTTTCAAAAAGGCTACAAACTCCATGACCGCATCCTACGCCCAGCAATGGTAGTGGTG
TATAACTAA60
120
180
240
300
360
420
480
540
549
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: spr0454 [new locus tag: SPR_RS02325 ]
- symbol: GrpE
- description: Heat-shock protein (activation of DnaK)
- length: 182
- theoretical pI: 4.33068
- theoretical MW: 20871.1
- GRAVY: -0.767582
⊟Function[edit | edit source]
- TIGRFAM: TraF-like protein (TIGR02740; HMM-score: 7.1)and 1 moreSH3 domain protein (TIGR04211; HMM-score: 5.3)
- TheSEED :
- Heat shock protein GrpE
and 1 more - PFAM: no clan defined GrpE; GrpE (PF01025; HMM-score: 147.6)and 2 moreGlyco_hydro_tim (CL0058) Glyco_hydro_17; Glycosyl hydrolases family 17 (PF00332; HMM-score: 15.4)no clan defined DUF763; Protein of unknown function (DUF763) (PF05559; HMM-score: 11.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004844
- TAT(Tat/SPI): 0.020091
- LIPO(Sec/SPII): 0.001531
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKSLKEANMAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFENKYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGLAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPGDDEHPVDTIAQVFQKGYKLHDRILRPAMVVVYN
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: hrcA > grpE > dnaK > dnaJ
⊟Regulation[edit | edit source]
- regulator: HrcA (repression) regulon
HrcA (TF) important in Heat shock response; RegPrecise transcription unit transferred from TIGR4 data RegPrecise
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0459 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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