Jump to navigation
Jump to search
PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BM6001serotype 19F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7465serotype 1
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 30-JAN-2014
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae R6
- locus tag: spr0554 [new locus tag: SPR_RS02795 ]
- pan locus tag?: PNEUPAN001523000
- symbol: spr0554
- pan gene symbol?: dacB
- synonym: ldcB
- product: Conserved hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: spr0554 [new locus tag: SPR_RS02795 ]
- symbol: spr0554
- product: Conserved hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 553724..554440
- length: 717
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: AE007317 (553724..554440) NCBI
- BioCyc: SPR0554 BioCyc
- MicrobesOnline: 133439 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_0549 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661ATGAAGAAAAGATACCTAGTCTTGACAGCTTTGCTAGCCTTGAGTCTAGCAGCTTGTTCA
CAAGAAAAAACAAAAAATGAAGATGGAGAAACTAAGACAGAACAGACAGCCAAAGCTGAT
GGAACAGTCGGTAGTAAGTCTCAAGGAGCTGCCCAGAAGAAAGCAGAAGTGGTCAATAAA
GGTGATTACTACAGCATTCAAGGGAAATACGATGAAATCATCGTAGCCAACAAACACTAT
CCATTGTCTAAAGACTATAATCCAGGGGAAAATCCAACAGCCAAGGCAGAGTTGGTCAAA
CTCATCAAAGCCATGCAAGAGGCAGGTTTCCCCATTAGCGATCATTACAGTGGTTTTAGA
AGTTATGAAACTCAGACCAAACTCTATCAAGATTATGTTAATCAAGATGGGAAAGAAGCT
GCCGACCGTTATTCTGCTCGACCTGGTTATAGCGAACACCAGACAGGCTTGGCCTTTGAT
GTGATTGGGACTGATGGTGATTTGGTAACAGAAGAAAAAGCAGCCCAATGGCTTTTGGAC
CATGCAGCTGATTATGGCTTTGTTGTCCGTTATCTCAAAGGTAAGGAAAAAGAAACAGGC
TATATGGCTGAGGAATGGCACCTGCGTTATGTCGGAAAAGAAGCAAAAGAAATTGCTGAG
AGTGGTCTCAGTTTGGAAGAATACTATGGCTTTGAAGGCGGAGACTACGTCGATTAA60
120
180
240
300
360
420
480
540
600
660
717
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: spr0554 [new locus tag: SPR_RS02795 ]
- symbol: spr0554
- description: Conserved hypothetical protein
- length: 238
- theoretical pI: 4.86696
- theoretical MW: 26538.2
- GRAVY: -0.744538
⊟Function[edit | edit source]
- TIGRFAM: Cell envelope Other uncharacterized lipoprotein (TIGR02722; HMM-score: 18.7)and 2 moreCellular processes Pathogenesis type VI secretion lipoprotein, VC_A0113 family (TIGR03352; HMM-score: 11.4)Protein fate Protein and peptide secretion and trafficking type VI secretion lipoprotein, VC_A0113 family (TIGR03352; HMM-score: 11.4)
- TheSEED :
- D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)
Cell Wall and Capsule Cell Wall and Capsule - no subcategory Murein Hydrolases D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)and 1 more - PFAM: Peptidase_MD (CL0170) VanY; D-alanyl-D-alanine carboxypeptidase (PF02557; HMM-score: 128.9)and 4 moreno clan defined DUF6491; Family of unknown function (DUF6491) (PF20101; HMM-score: 13.9)Myco_19_kDa; Mycobacterium 19 kDa lipoprotein antigen (PF05481; HMM-score: 13.8)Peptidase_MD (CL0170) Peptidase_M15_4; D-alanyl-D-alanine carboxypeptidase (PF13539; HMM-score: 13.8)Ferritin (CL0044) DUF6692; Domain of unknown function (DUF6692) (PF20402; HMM-score: 13)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.0013
- Cytoplasmic Membrane Score: 0.853
- Cell wall & surface Score: 0.0389
- Extracellular Score: 0.1067
- SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
- SP(Sec/SPI): 0.000523
- TAT(Tat/SPI): 0.00004
- LIPO(Sec/SPII): 0.999329
- Cleavage Site: CS pos: 18-19. LAA-CS. Pr: 0.9998
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVGSKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLIKAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFDVIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEIAESGLSLEEYYGFEGGDYVD
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription[edit | edit source]
- transcription start site:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0549 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Skye M Barendt, Lok-To Sham, Malcolm E Winkler
Characterization of mutants deficient in the L,D-carboxypeptidase (DacB) and WalRK (VicRK) regulon, involved in peptidoglycan maturation of Streptococcus pneumoniae serotype 2 strain D39.
J Bacteriol: 2011, 193(9);2290-300
[PubMed:21378199] [WorldCat.org] [DOI] (I p)Mohammed R Abdullah, Javier Gutiérrez-Fernández, Thomas Pribyl, Nicolas Gisch, Malek Saleh, Manfred Rohde, Lothar Petruschka, Gerhard Burchhardt, Dominik Schwudke, Juan A Hermoso, Sven Hammerschmidt
Structure of the pneumococcal l,d-carboxypeptidase DacB and pathophysiological effects of disabled cell wall hydrolases DacA and DacB.
Mol Microbiol: 2014, 93(6);1183-206
[PubMed:25060741] [WorldCat.org] [DOI] (I p)Juan Zhang, Yi-Hu Yang, Yong-Liang Jiang, Cong-Zhao Zhou, Yuxing Chen
Structural and biochemical analyses of the Streptococcus pneumonia L,D-carboxypeptidase DacB.
Acta Crystallogr D Biol Crystallogr: 2015, 71(Pt 2);283-92
[PubMed:25664738] [WorldCat.org] [DOI] (I p)