From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1032 [new locus tag: SPR_RS05145 ]
  • pan locus tag?: PNEUPAN002271000
  • symbol: glgA
  • pan gene symbol?: glgA
  • synonym:
  • product: Glycogen synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1032 [new locus tag: SPR_RS05145 ]
  • symbol: glgA
  • product: Glycogen synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1014454..1015887
  • length: 1434
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAAATTTTATTTGTAGCAGCAGAGGGTGCACCCTTTTCAAAAACAGGTGGTTTGGGA
    GACGTCATTGGCGCTCTTCCAAAATCACTGGTAAAAGCTGGGCACGAAGTTGCAGTGATT
    TTACCCTACTATGATATGGTAGAGGCTAAATTTGGAAATCAGATTGAAGATGTGCTTCAT
    TTTGAGGTGAGCGTTGGTTGGCGCAGACAGTATTGTGGAATTAAGAAAACAGTATTAAAT
    GGTGTAACCTTCTACTTTATTGACAATCAATATTATTTCTTCCGTGGTCATGTTTACGGT
    GATTTTGATGACGGAGAACGCTTTGCCTTTTTCCAACTGGCTGCCATTGAGGCTATGGAA
    AGGATTGACTTTATTCCTGATCTTCTCCATGTTCATGACTACCATACAGCTATGATTCCT
    TTCTTGTTGAAGGAAAAATACCGTTGGATTCAAGCCTATGAGGACATTGAAACAGTTTTA
    ACCATTCATAATTTAGAATTCCAAGGACAATTTTCAGAAGGAATGTTGGGTGATTTGTTT
    GGAGTTGGCTTTGAACGTTACGCTGATGGCACCCTTCGATGGAACAACTGTCTGAACTGG
    ATGAAGGCAGGTATTCTCTATGCGAACCGTGTTTCAACCGTTTCACCTAGCTATGCTCAT
    GAAATTATGACTAGTCAGTTTGGATGTAATTTGGATCATATTCTTAAAATGGAGTCTGGT
    AAAGTATCTGGTATCGTGAATGGGATTGATGCTGATCTTTATAATCCTCAGACGGATGCT
    CTTTTAGACTATCATTTCAATCAGGAAGATTTGTCTGGGAAAGCCAAAAATAAGGCAAAA
    TTGCAAGAAAGAGTTGGCTTGCCTGTTAGAGCAGACGTTCCACTGGTGGGAATTGTTTCT
    CGTTTGACACGTCAAAAAGGTTTTGATGTGGTGGTCGAAAGTCTTCACCATATCTTGCAA
    GAAGATGTTCAGATTGTTCTTTTGGGAACTGGCGATCCAGCCTTTGAAGGAGCTTTCTCA
    TGGTTTGCTCAGATTTACCCAGACAAGCTATCAACAAATATCACTTTTGATGTCAAACTT
    GCTCAGGAAATCTACGCTGCTTGTGACCTCTTCCTCATGCCAAGTCGTTTTGAACCGTGT
    GGCTTGTCTCAAATGATGGCTATGCGTTATGGAACCTTGCCATTGGTCCATGAAGTTGGA
    GGCTTGCGAGATACAGTTCGCGCTTTCAATCCAATCGAAGGAAGCGGTACTGGCTTTAGC
    TTTGACAATCTATCTCCTTATTGGTTAAATTGGACTTTCCAAACAGCATTGGACTTGTAT
    AGAAACCATCCAGACATTTGGAGAAACCTACAAAAACAAGCTATGGAGAGTGACTTCTCA
    TGGGATACAGCCTGCAAGTCATACCTTGACTTGTACCATAGTTTAGTTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1434

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1032 [new locus tag: SPR_RS05145 ]
  • symbol: GlgA
  • description: Glycogen synthase
  • length: 477
  • theoretical pI: 5.07901
  • theoretical MW: 54167.4
  • GRAVY: -0.0828092

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.21?  ExPASy
    Starch synthase ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1)
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 618.3)
    and 6 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 54.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 34.5)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 31.4)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 28.1)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 21.6)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 17.5)
  • TheSEED  :
    • Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)
    Carbohydrates Polysaccharides Glycogen metabolism  Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)
  • PFAM:
    GT-B (CL0113) Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 272.9)
    and 4 more
    Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 50.2)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 42.5)
    Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 35.3)
    Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 26)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019569
    • TAT(Tat/SPI): 0.000957
    • LIPO(Sec/SPII): 0.002767
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYYDMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDFDDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVLTIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSYAHEIMTSQFGCNLDHILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSWDTACKSYLDLYHSLVN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=spr1032&oldid=129691"