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serotype 4
D39
serotype 2
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serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1239 [new locus tag: SPR_RS06195 ]
  • pan locus tag?: PNEUPAN002707000
  • symbol: amy
  • pan gene symbol?: amy
  • synonym:
  • product: Alpha-amylase precursor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1239 [new locus tag: SPR_RS06195 ]
  • symbol: amy
  • product: Alpha-amylase precursor
  • replicon: chromosome
  • strand: +
  • coordinates: 1237343..1238797
  • length: 1455
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAAAATCAAACACTCATGCAATACTTTGAATGGTATCTGCCCCACGACGGTCAACAC
    TGGACGCGTCTGGCTGAAAATGCTCCACACCTAGCTCATCTGGGGATCAGTCACGTCTGG
    ATGCCACCAGCCTTCAAGGCAACCAACGAAAAAGATGTCGGCTATGGGGTCTATGACTTA
    TTTGACTTAGGAGAGTTCAACCAAAAAGGGACTGTCCGCACCAAGTATGGTTTCAAAGAA
    GACTATCTTCAAGCCATTCAAGCCCTTAAAGCACAGGGAATTCAACCTATGGCCGATGTA
    GTTCTCAACCACAAGGCTGCTGCCGATCACAGGGAAGCCTTTCAGGTTATCGAAGTTGAT
    CCTGTAGACCGTACAGTTGAACTTGGAGAACCCTTCACCATCAATGGCTGGACTAGTTTT
    ACCTTCGATGGTCGCCAAGATACCTATAATGGCTTCCACTGGCATTGGTACCACTTCACC
    GGTACAGACTACGATGCCAAACGCAGTAAATCTGGGATTTATCTGATCCAAGGGGACAAC
    AAGGGCTGGGCTAACGAGGAATTGGTCGATAACGAAAACGGAAACTACGACTACCTCATG
    TATGCCGACCTAGACTTTAAACATCCTGAAGTCATCCAAAACATCTATGACTGGGCTGAT
    TGGTTCATGGAAACGACTGGTGTAGCTGGTTTCCGTTTGGATGCCGTTAAGCATATTGAC
    TCTTTCTTTATGCGCAACTTCATCCGCGATATGAAGGAAAAATACGGTGACGATTTCTAT
    GTTTTTGGTGAATTTTGGAACTCAGACAAGGAAGCCAATCTGGACTATCTCGAAAAAACG
    GAAGAACACTTTGACCTTGTCGATGTTCGTCTCCACCAGAATCTCTTTGAAGCCAGTCAA
    GCTGGCGCAAACTATGACCTTCGTGGCATTTTCACAGATAGCCTGGTTGAACTCAAGCCT
    GACAAGGCTGTGACTTTTGTCGACAACCACGATACCCAACGAGGACAAGCCCTTGAGTCT
    ACCGTTGAAGAATGGTTCAAGCCAGCAGCCTATGCCCTCATTTTGTTACGCCAAGACGGC
    CTTCCATGTGTCTTTTACGGAGACTACTATGGGATTTCAGGGCAGTATGCTCAACAAGAT
    TTCAAAGAAATCCTTGACCGCCTCCTAGCCATCCGAAAAGACTTGGCCTATGGAGAACAA
    AATGACTACTTTGACCATGCTAACTGTATCGGTTGGGTACGTTCAGGTGCTGAAAATCAA
    TCCCCAATCGCAGTCCTTATCTCAAATGACCAAGAAAACAGCAAGTCAATGTTTGTCGGT
    CAAGAATGGACTAATCAAACCTTTGTAGATTTACTTGGTAGCCACCAAGGTCAAGTTACA
    ATTGATGAGGAAGGTTATGGACAATTCCCTGTCTCAGCTAGATCCGTAAGTGTCTGGGCA
    GTCAATACCATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1455

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1239 [new locus tag: SPR_RS06195 ]
  • symbol: Amy
  • description: Alpha-amylase precursor
  • length: 484
  • theoretical pI: 4.43607
  • theoretical MW: 55879.4
  • GRAVY: -0.522521

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.1?  ExPASy
    Alpha-amylase Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 45.8)
    and 4 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 28.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 27)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 19.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 11.6)
  • TheSEED  :
    • Cytoplasmic alpha-amylase (EC 3.2.1.1)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Cytoplasmic alpha-amylase (EC 3.2.1.1)
    and 1 more
    Carbohydrates Polysaccharides Glycogen metabolism  Cytoplasmic alpha-amylase (EC 3.2.1.1)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 115.3)
    and 2 more
    GHD (CL0369) Alpha-amy_C_pro; Alpha-amylase C-terminal (PF09154; HMM-score: 47.8)
    Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 40.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016868
    • TAT(Tat/SPI): 0.000757
    • LIPO(Sec/SPII): 0.001168
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVLNHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFTGTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNSDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIRKDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGSHQGQVTIDEEGYGQFPVSARSVSVWAVNTI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MalR (repression) regulon
    MalR(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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