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serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1515 [new locus tag: SPR_RS07515 ]
  • pan locus tag?: PNEUPAN003087000
  • symbol: ddl
  • pan gene symbol?: ddl
  • synonym:
  • product: D-alanine-D-alanine ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1515 [new locus tag: SPR_RS07515 ]
  • symbol: ddl
  • product: D-alanine-D-alanine ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1492390..1493433
  • length: 1044
  • essential: yes [1] DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAACAAACGATTATTCTTTTATATGGTGGACGGAGTGCGGAACGCGAAGTCTCTGTC
    CTTTCAGCTGAGAGTGTCATGCGTGCGGTCGATTACGACCGTTTCACAGTCAAGACTTTC
    TTTATCAGTCAGTCAGGTGACTTTATCAAAACACAGGAATTTAGTCATGCTCCGGGGCAA
    GAAGACCGTCTCATGACCAATGAAACCATTGATTGGGATAAGAAAGTTGCACCAAGTGCT
    ATCTACGAAGAAGGTGCAGTGGTCTTTCCAGTCCTTCACGGGCCAATGGGAGAAGATGGC
    TCTGTTCAAGGATTCTTGGAAGTTTTGAAAATGCCTTACGTTGGTTGCAACATTTTGTCA
    TCAAGTCTTGCCATGGATAAAATCACGACTAAGCGTGTTCTGGAATCTGCTGGTATTGCC
    CAAGTTCCTTATGTGGCTATCGTTGAAGGCGATGATGTGACTGCTAAAATCGCTGAAGTG
    GAAGAAAAATTGGCTTATCCAGTCTTCACTAAGCCGTCAAACATGGGGTCTAGTGTCGGT
    ATTTCTAAGTCTGAAAACCAAGAAGAACTCCGTCAAGCCTTAAAACTTGCCTTCCGATAT
    GACAGCCGTGTCTTGGTTGAGCAAGGAGTGAATGCCCGTGAAATTGAGGTTGGCCTCTTG
    GGTAACTACGATGTCAAGAGCACGCTACCTGGAGAAGTTGTCAAGGACGTTGCCTTTTAT
    GACTACGATGCCAAGTATATTGATAACAAGGTTACTATGGATATTCCTGCCAAAATCAGT
    GATGATGTGGTGGCTGTCATGCGTCAAAATGCAGAAACAGCCTTCCGTGCCATTGGTGGC
    CTTGGTCTATCTCGTTGCGATTTCTTCTATACAGATAAGGGAGAGATTTTTCTCAACGAG
    CTCAATACCATGCCAGGTTTCACCCAGTGGTCTATGTACCCACTACTTTGGGAAAATATG
    GGGATCAGCTACCCAGAACTAATCGAGCGTTTGGTTGACCTTGCCAAGGAAAGTTTTGAC
    AAGCGCGAAGCGCATTTGATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1515 [new locus tag: SPR_RS07515 ]
  • symbol: Ddl
  • description: D-alanine-D-alanine ligase
  • length: 347
  • theoretical pI: 4.46741
  • theoretical MW: 38717.9
  • GRAVY: -0.129971

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.4?  ExPASy
    D-alanine--D-alanine ligase ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 332.4)
    and 12 more
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 34.4)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 29.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 27)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 26.2)
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 25.1)
    GNAT-family acetyltransferase TIGR03103 (TIGR03103; HMM-score: 19.7)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 18.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs glutamate--cysteine ligase/glutathione synthase (TIGR01435; EC 6.3.2.2,6.3.2.3; HMM-score: 17.8)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 16.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme gamma-F420-2:alpha-L-glutamate ligase (TIGR04443; EC 6.3.2.32; HMM-score: 15.3)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 14.8)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 11.8)
  • TheSEED  :
    • D-alanine--D-alanine ligase (EC 6.3.2.4)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  D-alanine--D-alanine ligase (EC 6.3.2.4)
    and 2 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan biosynthesis--gjo  D-alanine--D-alanine ligase (EC 6.3.2.4)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan biosynthesis--gjo  D-alanine--D-alanine ligase A (EC 6.3.2.4)
  • PFAM:
    ATP-grasp (CL0179) Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 241.6)
    and 4 more
    PreATP-grasp (CL0483) Dala_Dala_lig_N; D-ala D-ala ligase N-terminus (PF01820; HMM-score: 95.8)
    ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 34.4)
    CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 27.7)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 16)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9804
    • Cytoplasmic Membrane Score: 0.0025
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0169
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012417
    • TAT(Tat/SPI): 0.000658
    • LIPO(Sec/SPII): 0.001744
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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