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D39V
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Hungary19A-6
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EF3030
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670-6B
serotype 6B
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70585
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A026
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A66
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AP200
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ATCC 49619
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HU-OH
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Hu15
serotype 19A
Hu17
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INV104
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INV200
serotype 14
JJA
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MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
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R6
serotype 2
SP49
serotype 19A
SPN032672
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SPN034156
serotype 3
SPN034183
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SPN994038
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SPN994039
serotype 3
SPNA45
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ST556
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TCH8431/19A
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Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1945 [new locus tag: SPR_RS09920 ]
  • pan locus tag?: PNEUPAN003823000
  • symbol: pcpA
  • pan gene symbol?: cbpN
  • synonym:
  • product: Choline-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1945 [new locus tag: SPR_RS09920 ]
  • symbol: pcpA
  • product: Choline-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1925783..1927855
  • length: 2073
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAAAAAAACTACAATATTATCATTAACTACAGCTGCGGTTATTTTAGCAGCATATGTC
    CCTAATGAACCAATCCTAGCAGCATATGTCCCTAATGAACCAATCCTAGCAGATACTCCT
    AGTTCGGAAGTAATCAAAGAGACTAAAGTTGGAAGTATTATTCAACAAAATAATATCAAA
    TATAAGGTTCTAACTGTAGAAGGTAACATAGGAACTGTTCAAGTGGGTAATGGAGTTACT
    CCTGTAGAGTTTGAAGCTGGTCAAGATGGAAAACCATTCACGATTCCTACAAAAATCACA
    GTAGGTGATAAAGTATTTACCGTTACTGAAGTAGCTAGTCAAGCTTTTAGTTATTATCCA
    GATGAAACAGGTAGAATTGTCTACTATCCTAGCTCTATTACTATCCCATCAAGCATAAAA
    AAAATACAAAAAAAAGGCTTCCATGGAAGTAAAGCTAAAACTATTATTTTTGACAAAGGC
    AGTCAGCTGGAGAAAATTGAAGATAGAGCTTTTGATTTTTCTGAATTAGAAGAGATTGAA
    TTGCCTGCATCTCTAGAATATATTGGAACAAGTGCATTTTCTTTTAGTCAAAAATTGAAA
    AAGCTAACCTTTTCCTCAAGTTCAAAATTAGAATTAATATCACATGAGGCTTTTGCTAAT
    TTATCAAATTTAGAGAAACTAACATTACCAAAATCGGTTAAAACATTAGGAAGTAATCTA
    TTTAGACTCACTACTAGCTTAAAACATGTTGATGTTGAAGAAGGAAATGAATCGTTTGCC
    TCAGTTGATGGTGTTTTGTTTTCAAAAGATAAAACTCAATTAATTTATTATCCAAGTCAA
    AAAAATGACGAAAGTTATAAAACGCCTAAGGAGACAAAAGAACTTGCATCATATTCGTTT
    AATAAAAATTCTTACTTGAAAAAACTCGAATTGAATGAAGGTTTAGAAAAAATCGGTACT
    TTTGCATTTGCGGATGCGATTAAACTTGAAGAAATTAGCTTACCAAATAGTTTAGAAACT
    ATTGAACGTTTAGCCTTTTACGGTAATTTAGAATTAAAAGAACTTATATTACCAGATAAT
    GTTAAAAATTTTGGTAAACACGTTATGAACGGTTTACCAAAATTTTTAACATTATCTGGT
    AATAATATCAACTCATTGCCGTCCTTCTTCCTAAGTGGCGTCTTAGATTCATTAAAGGAA
    ATTCATATTAAGAATAAAAGTACAGAGTTTTCTGTGAAAAAAGATACATTTGCAATTCCT
    GAAACTGTTAAGTTCTATGTAACATCAGAACATATAAAAGATGTTCTTAAATCAAATTTA
    TCTACTAGTAATGATATCATTGTTGAAAAAGTAGATAATATAAAACAAGAAACTGATGTA
    GCTAAACCTAAAAAGAATTCTAATCAGGGAGTAGTTGGTTGGGTTAAAGACAAAGGTTTA
    TGGTATTACTTAAACGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGTTTA
    TGGTATTACTTAAACGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGTTTA
    TGGTATTACTTAAATGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGCTTA
    TGGTATTACTTAAACGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGCTTA
    TGGTATTACTTAAACGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGCTTA
    TGGTATTACTTAAATGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGCTTA
    TGGTATTACTTAAACGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGCTTA
    TGGTATTACTTAAACGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGACAAAGGCTTA
    TGGTATTACTTAAATGAATCAGGTTCAATGGCTACTGGTTGGGTTAAAGTTTCTGGTAAA
    TGGTACTATACCTATAATTCAGGAGATTTATTAGTAAACACGACTACACCCGATGGCTAT
    CGAGTCAATGCTAACGGTGAGTGGGTAGGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2073

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1945 [new locus tag: SPR_RS09920 ]
  • symbol: PcpA
  • description: Choline-binding protein
  • length: 690
  • theoretical pI: 6.82893
  • theoretical MW: 77100.9
  • GRAVY: -0.347536

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 41)
  • TheSEED  :
    • Choline binding protein PcpA
  • PFAM:
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 211.9)
    Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 180.7)
    LRR (CL0022) LRR_5; BspA type Leucine rich repeat region (6 copies) (PF13306; HMM-score: 177.1)
    and 3 more
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 60.4)
    LRR (CL0022) LRR_8; Leucine Rich Repeat (PF13855; HMM-score: 35.1)
    LRR_4; Leucine Rich repeats (2 copies) (PF12799; HMM-score: 17.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0006
    • Cytoplasmic Membrane Score: 0.017
    • Cell wall & surface Score: 0.1028
    • Extracellular Score: 0.8796
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.972584
    • TAT(Tat/SPI): 0.003663
    • LIPO(Sec/SPII): 0.011626
    • Cleavage Site: CS pos: 27-28. ILA-AY. Pr: 0.8750
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKTTILSLTTAAVILAAYVPNEPILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELILPDNVKNFGKHVMNGLPKFLTLSGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Laura Selva, Pilar Ciruela, Krystle Blanchette, Eva del Amo, Roman Pallares, Carlos J Orihuela, Carmen Muñoz-Almagro
Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae.
PLoS One: 2012, 7(7);e41587
[PubMed:22848535] [WorldCat.org] [DOI] (I p)

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