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serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1990 [new locus tag: SPR_RS10140 ]
  • pan locus tag?: PNEUPAN003845000
  • symbol: spr1990
  • pan gene symbol?: glpO
  • synonym:
  • product: Glycerol-3-phosphate dehydrogenase, truncation

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1990 [new locus tag: SPR_RS10140 ]
  • symbol: spr1990
  • product: Glycerol-3-phosphate dehydrogenase, truncation
  • replicon: chromosome
  • strand: -
  • coordinates: 1980789..1982174
  • length: 1386
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAATTTTCAAAAAAAACACGTGAATTGTCAATTAAAAAAATGCAGGAACGCACCCTG
    GACCTCTTGATTATCGGTGGAGGAATCACAGGAGCTGGTGTAGCCTTGCAGGCGGCAGCT
    AGCGGTCTTGAGACTGGTTTGATTGAAATGCAAGACTTTGCAGAAGGAACATCTAGTCGT
    TCAACAAAATTGGTTCACGGAGGTCTTCGCTACCTCAAACAATTCGACGTAGAAGTGGTC
    TCAGATACGGTTTCTGAACGTGCAGTGGTTCAACAAATCGCTCCACACATTCCAAAACCA
    GATCCAATGCTCTTACCAGTTTACGATGAAGATGGAGCAACCTTTAGCCTCTTCCGTCTT
    AAAGTAGCCATGGACTTGTACGACCTCTTGGCAGGTGTTAGCAACACACCAACTGCGAAC
    AAGGTTTTGAGCAAGGATCAAGTCTTGGAACGCCAGCCAAACTTGAAGAAGGAAGGCTTG
    GTAGGAGGTGGAGTGTATCTTGACTTCCGTAACAACGATGCGCGTCTCGTGATTGAAAAC
    ATCAAACGTGCCAACCAAGACGGTGCCCTCATTGCCAACCACGTGAAGGCAGAAGGCTTC
    CTCTTTGACGAAAGTGGCAAGATTACAGGTGTTGTAGCTCGTGATCTCTTGACAGACCAA
    GTATTTGAAATCAAGGCCCGTCTGGTTATTAATACAACAGGTCCTTGGAGTGATAAAGTA
    CGTAATTTGTCTAATAAGGGAACGCAATTCTCACAAATGCGCCCAACTAAGGGAGTTCAC
    TTGGTAGTGGATTCAAGCAAAATCAAGGTTTCACAGCCAGTTTACTTTGACACAGGTTTG
    GGTGACGGTCGTATGGTCTTTGTTCTCCCACGTGAAAACAAGACTTACTTTGGTACAACT
    GATACAGACTACACAGGTGATTTGGAGCATCCAAAAGTAACTCAAGAAGATGTAGATTAT
    CTACTTGGCATTGTCAACAACCGCTTTCCAGAATCCAACATCACCATTGATGATATCGAA
    AGCAGCTGGGCAGGTCTTCGTCCATTGATTGCAGGGAACAGTGCCTCTGACTATAATGGT
    GGAAATAACGGTACCATCAGTGATGAAAGCTTTGACAACTTGATTGCGACTGTTGAATCT
    TATCTCTCCAAAGAAAAAACACGTGAAGATGTTGAGTCTGCTGTCAGCAAGCTTGAAAGT
    AGCACATCTGAGAAACATTTGGATCCATCTGCAGTTTCTCGTGGGTCTAGCTTGGATCGT
    GATGACAATGGTCTTTTGACTCTTGCTGGCGGTAAAATCACAGACTACCGTAAGATGGGT
    GACGAGGCGCTATGGAGCGCGTGGTTGACATCCTCAAAGCAGAATTTGACCGTAGCTTTA
    AATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1990 [new locus tag: SPR_RS10140 ]
  • symbol: spr1990
  • description: Glycerol-3-phosphate dehydrogenase, truncation
  • length: 461
  • theoretical pI: 4.90629
  • theoretical MW: 50415.4
  • GRAVY: -0.34013

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, A subunit (TIGR03377; EC 1.1.5.3; HMM-score: 139.8)
    and 17 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 33.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 22.5)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 22.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 18.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 17.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 17.7)
    lycopene cyclase family protein (TIGR01790; HMM-score: 15.2)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 14.3)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 14.3)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 13.5)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 12.7)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 12.3)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.3)
    Metabolism Energy metabolism Anaerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.3)
    Metabolism Energy metabolism Aerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.3)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 12.3)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 10.2)
  • TheSEED  :
    • Glycerol-3-phosphate oxidase (EC 1.1.3.21)
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Alpha-glycerophosphate oxidase (EC 1.1.3.21)
    and 1 more
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alpha-glycerophosphate oxidase (EC 1.1.3.21)
  • PFAM:
    NADP_Rossmann (CL0063) DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 189.4)
    and 10 more
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 22.6)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 21.3)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 21.2)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 16.3)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 16.1)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 13.3)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 13.2)
    Saposin_like (CL0707) SapB_1; Saposin-like type B, region 1 (PF05184; HMM-score: 11.4)
    NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 10.9)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • SignalP: Signal peptide TAT(Tat/SPI) length 40 aa
    • SP(Sec/SPI): 0.23359
    • TAT(Tat/SPI): 0.486981
    • LIPO(Sec/SPII): 0.014889
    • Cleavage Site: CS pos: 40-41. AAA-SG. Pr: 0.3443
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLETGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDPMLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGLVGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLTDQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYFDTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNITIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVESAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMGDEALWSAWLTSSKQNLTVALN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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