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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7466
  • locus tag: DQM66_RS03065 [old locus tag: NCTC7466_00587 ]
  • pan locus tag?: PNEUPAN001559000
  • symbol: DQM66_RS03065
  • pan gene symbol?: ytqB
  • synonym:
  • product: tRNA (mnm(5)s(2)U34)-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: DQM66_RS03065 [old locus tag: NCTC7466_00587 ]
  • symbol: DQM66_RS03065
  • product: tRNA (mnm(5)s(2)U34)-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 589502..590059
  • length: 558
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_LS483374 (589502..590059) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0567 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAAAGACCACTTGAGATGGCACATGATTTTTTGGCTGAGGTCGTGACAAAAGAGGAT
    GTCGTAGTGGATGCGACTATGGGAAATGGTCATGACACGCTTTTTTTAGCCAAGCTAGCC
    AAGCAAGTCTATGCCTTTGATATTCAGAAGCAAGCCTTGGAAAAGACCCAAGAGCGTTTG
    CATCAGGCTGGCTTGACAAATGCCCAGTTAATCTTGCAAGGCCATGAGACACTGGACCAG
    TTTGTGACAAAAGCTAAGGCAGGGATTTTTAATTTGGGCTATTTGCCGTCAGCTGATAAG
    TCTGTCATCACCCGACCGCAGACAACGATTGAGGCATTAGAAAAGCTATGTGGCTTACTT
    GTCAAGGGTGGACGAATTGCTATTATGATTTACTATGGTCATGAAGGAGGCGACCTCGAG
    AGAGATGCTGTCTTGGATTTTGTAATCCAGTTGAACCAACAAGAGTACACAGCTGCCATT
    TACCGAACTTTAAACCAAGTCAACAACCCGCCGTTTTTAGTGATGATTGAAAAATTAGAG
    AGATACAGACATGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: DQM66_RS03065 [old locus tag: NCTC7466_00587 ]
  • symbol: DQM66_RS03065
  • description: tRNA (mnm(5)s(2)U34)-methyltransferase
  • length: 185
  • theoretical pI: 6.51567
  • theoretical MW: 20812.8
  • GRAVY: -0.168108

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.61?  ExPASy
    tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 32.4)
    and 9 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 18.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (uracil(54)-C(5))-methyltransferase (TIGR02143; EC 2.1.1.35; HMM-score: 16.7)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 16.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 15.7)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 14)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 13.9)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 13.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 12.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 11.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 166.5)
    and 18 more
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 33)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 31.3)
    Methyltransf_5; MraW methylase family (PF01795; HMM-score: 23.7)
    Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 20.7)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 18)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 17.8)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 17.2)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 17.1)
    Met_10; Met-10+ like-protein (PF02475; HMM-score: 16.4)
    tRNA_U5-meth_tr; tRNA (Uracil-5-)-methyltransferase (PF05958; HMM-score: 16.3)
    Methyltrans_SAM; S-adenosylmethionine-dependent methyltransferase (PF10672; HMM-score: 15.1)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 14.9)
    N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 14.8)
    RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 14.1)
    Methyltransf_33; Histidine-specific methyltransferase, SAM-dependent (PF10017; HMM-score: 13.3)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 13)
    PDDEXK (CL0236) ERCC4; ERCC4 domain (PF02732; HMM-score: 12.2)
    NADP_Rossmann (CL0063) TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9968
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0.0012
    • Extracellular Score: 0.001
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003319
    • TAT(Tat/SPI): 0.000488
    • LIPO(Sec/SPII): 0.000355
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000828435 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVYAFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSVITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAIYRTLNQVNNPPFLVMIEKLERYRHG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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