⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae EF3030
- locus tag: EF3030_09720 [new locus tag: EF3030_RS09720 ]
- pan locus tag?: PNEUPAN003543000
- symbol: EF3030_09720
- pan gene symbol?: rr11
- synonym:
- product: response regulator transcription factor
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: EF3030_09720 [new locus tag: EF3030_RS09720 ]
- symbol: EF3030_09720
- product: response regulator transcription factor
- replicon: chromosome
- strand: -
- coordinates: 1838611..1839210
- length: 600
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: CP035897 (1838611..1839210) NCBI
- BioCyc: see EF3030_RS09720
- MicrobesOnline:
- PneumoBrowse for strain D39V: SPV_1798 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGAAAGTATTAGTCGCAGAAGATCAAAGTATGTTGCGAGATGCCATGTGTCAATTGCTC
ACGCTTCAACCAGATGTAGAGTCTGTCTTACAAGCCAAGAATGGGCAAGAAGCAATCCAA
CTATTAGAAAAGGAGTCTGTAGATATCGCCATCCTTGACGTAGAAATGCCTGTTAAGACA
GGTCTTGAAGTCTTGGAGTGGATACGATCAGAAAAGCTTGAAACAAAGGTGGTTGTGGTG
ACGACCTTCAAGCGTGCTGGGTATTTTGAACGTGCGGTCAAGGCTGGAGTGGATGCTTAT
GTATTAAAGGAACGAAGCATTGCAGACCTCATGCAAACCTTGCACACCGTCCTCGAAGGA
CGCAAGGAGTATTCGCCTGAATTGATGGAAGTGGTGATGACCCGTCCCAATCCGTTGACA
GAACAAGAGATTGCTGTCTTAAAGGGAATTGCCCGGGGCTTATCCAACCAAGAAATCGCA
GATCAGCTTTACCTCTCAAACGGAACTATTCGAAACTATGTCACCAATATTCTTTCAAAA
CTGGATGCTGGTAATCGAACAAAGGCAGCTAATATCGCAAAAGAATCTGGTTGGTTATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: EF3030_09720 [new locus tag: EF3030_RS09720 ]
- symbol: EF3030_09720
- description: response regulator transcription factor
- length: 199
- theoretical pI: 4.84475
- theoretical MW: 22232.6
- GRAVY: -0.11809
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 61.9)and 15 moreRegulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 42.9)Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 42.9)Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 42.5)transcriptional regulator EpsA (TIGR03020; HMM-score: 42.1)Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 32.3)Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 31.6)Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 31.6)Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 31.6)LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 23.3)proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 20.9)RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.6)RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 19.5)Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 18.5)Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 16.7)Regulatory functions DNA interactions DNA-binding protein, Tfx family (TIGR00721; HMM-score: 14.2)
- TheSEED: data available for D39, Hungary19A-6, TIGR4
- PFAM: CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 87.6)and 11 moreHTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 64.3)CheY (CL0304) cREC_REC; Cyclic-phosphate processing Receiver domain (PF20274; HMM-score: 21.7)HTH (CL0123) Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 18)HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18)HTH_11; HTH domain (PF08279; HMM-score: 16)HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.4)PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 14.4)HTH (CL0123) Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 14.3)HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 14)P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 12.5)HTH (CL0123) HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003965
- TAT(Tat/SPI): 0.000206
- LIPO(Sec/SPII): 0.000541
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: QBF69709 NCBI
- UniProt:
⊟Protein sequence[edit | edit source]
- MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEKESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVLKERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIADQLYLSNGTIRNYVTNILSKLDAGNRTKAANIAKESGWL
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1798 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Wolfgang Haas, Deepak Kaushal, Jack Sublett, Caroline Obert, Elaine I Tuomanen
Vancomycin stress response in a sensitive and a tolerant strain of Streptococcus pneumoniae.
J Bacteriol: 2005, 187(23);8205-10
[PubMed:16291696] [WorldCat.org] [DOI] (P p)Riana Cockeran, Jenny A Herbert, Timothy J Mitchell, Thérèse Dix-Peek, Caroline Dickens, Ronald Anderson, Charles Feldman
Exposure of a 23F serotype strain of Streptococcus pneumoniae to cigarette smoke condensate is associated with selective upregulation of genes encoding the two-component regulatory system 11 (TCS11).
Biomed Res Int: 2014, 2014;976347
[PubMed:25013815] [WorldCat.org] [DOI] (I p)