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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-MAY-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_2333 [new locus tag: HKM25_RS11355 ]
  • pan locus tag?: PNEUPAN003934000
  • symbol: HKM25_2333
  • pan gene symbol?: comD
  • synonym:
  • product: histidine kinase-, DNA gyrase B-, and HSP90-like ATPase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_2333 [new locus tag: HKM25_RS11355 ]
  • symbol: HKM25_2333
  • product: histidine kinase-, DNA gyrase B-, and HSP90-like ATPase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2215349..2216290
  • length: 942
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP053210 (2215349..2216290) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_2064 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGTTAACCTATTTTGTTACATTAAAAATCATTTACTATTTCCATTTGGAACTAGCCTAT
    TTTGACAAGGACTACCTTTATCCTTTCTTGAAAAAAGTATTTTTTGCTTTACTATTGCTA
    CATATTGTATCTTTCGTTTCAGATATGGTAAGTACGATTAAACATTTGAATAGTTTTGGA
    AGTATTTTGTCATCTATTGTCTTTATCTCTCTCCTTTTGACCTTCTTTGCAATGAATTCT
    CATAAAGTTCAAATGGAGAAAGAGATTGCTTTGAAGCAGAAGAAATTTGAACAGAAACAT
    TTACAGAATTACACAGATGAAATTGTTGGTCTGTATAATGAAATCCGTGGTTTTCGACAT
    GATTATGCTGGAATGCTTGTCAGCATGCAGATGGCAATTGACAGTGGTAATTTACAGGAA
    ATTGACAGAATTTACAATGAAGTTTTAGTCAAAGCAAATCATAAATTGCGTTCAGATAAG
    TACACTTACTTTGATTTGAACAACATAGAAGACTCAGCTTTACGAAGTTTGGTTGCTCAG
    TCAATTGTCTATGCTCGAAATAATGGTGTAGAGTTTACACTGGAAGTAAAAGATACGATT
    ACCAAGCTTCCAATTGAACTATTGGATTTGGTTCGTATCATGAGCGTTTTATTGAATAAT
    GCTGTCGAAGGATCGGCTGATAGCTATAAAAAGCAGATGGAAGTAGCAGTTATTAAGATG
    GAAACTGAAACAGTTATTGTGATTCAGAATTCATGTAAAATGACGATGACTCCTTCAGGA
    GATCTATTTGCCTTAGGATTCTCCACTAAGGGAAGAAATCGCGGAGTCGGATTAAATAAT
    GTGAAAGAACTACTAGATAAGTACAACAATATTATTTTAGAAACAGAGATGGAAGGCAGT
    ACATTTAGACAAATCATTAGATTTAAGAGGGAATTTGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_2333 [new locus tag: HKM25_RS11355 ]
  • symbol: HKM25_2333
  • description: histidine kinase-, DNA gyrase B-, and HSP90-like ATPase family protein
  • length: 313
  • theoretical pI: 7.23151
  • theoretical MW: 36213.9
  • GRAVY: 0.0217252

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation integral membrane protein YtvI (TIGR02872; HMM-score: 17.3)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 52.8)
    and 5 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 28.7)
    Peptidase_MA (CL0126) DUF3810; Protein of unknown function (DUF3810) (PF12725; HMM-score: 19.1)
    no clan defined DUF6172; Family of unknown function (DUF6172) (PF19669; HMM-score: 13.4)
    Sialidase (CL0434) Mcl1_mid; Minichromosome loss protein, Mcl1, middle region (PF12341; HMM-score: 12.1)
    no clan defined DUF1514; Protein of unknown function (DUF1514) (PF07438; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 2
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003193
    • TAT(Tat/SPI): 0.00009
    • LIPO(Sec/SPII): 0.001005
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: QJS37979 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLTYFVTLKIIYYFHLELAYFDKDYLYPFLKKVFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

O Ween, P Gaustad, L S Håvarstein
Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.
Mol Microbiol: 1999, 33(4);817-27
[PubMed:10447890] [WorldCat.org] [DOI] (P p)Bernard Martin, Chantal Granadel, Nathalie Campo, Vincent Hénard, Marc Prudhomme, Jean-Pierre Claverys
Expression and maintenance of ComD-ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae.
Mol Microbiol: 2010, 75(6);1513-28
[PubMed:20180906] [WorldCat.org] [DOI] (I p)Claudia Trappetti, Luciana Gualdi, Lorenzo Di Meola, Prashant Jain, Cindy C Korir, Paul Edmonds, Francesco Iannelli, Susanna Ricci, Gianni Pozzi, Marco R Oggioni
The impact of the competence quorum sensing system on Streptococcus pneumoniae biofilms varies depending on the experimental model.
BMC Microbiol: 2011, 11;75
[PubMed:21492426] [WorldCat.org] [DOI] (I e)M S Chandler, D A Morrison
Competence for genetic transformation in Streptococcus pneumoniae: molecular cloning of com, a competence control locus.
J Bacteriol: 1987, 169(5);2005-11
[PubMed:3032904] [WorldCat.org] [DOI] (P p)L S Håvarstein, G Coomaraswamy, D A Morrison
An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
Proc Natl Acad Sci U S A: 1995, 92(24);11140-4
[PubMed:7479953] [WorldCat.org] [DOI] (P p)E V Pestova, L S Håvarstein, D A Morrison
Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system.
Mol Microbiol: 1996, 21(4);853-62
[PubMed:8878046] [WorldCat.org] [DOI] (P p)L S Håvarstein, P Gaustad, I F Nes, D A Morrison
Identification of the streptococcal competence-pheromone receptor.
Mol Microbiol: 1996, 21(4);863-9
[PubMed:8878047] [WorldCat.org] [DOI] (P p)

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