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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-MAY-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_257 [new locus tag: HKM25_RS01260 ]
  • pan locus tag?: PNEUPAN000978000
  • symbol: HKM25_257
  • pan gene symbol?: pflE
  • synonym:
  • product: glycyl-radical enzyme activating family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_257 [new locus tag: HKM25_RS01260 ]
  • symbol: HKM25_257
  • product: glycyl-radical enzyme activating family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 225515..226156
  • length: 642
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP053210 (225515..226156) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0229 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAACCTGAAAAAATGAAAGATGCTCAACGAGAGAAATTCACCTTAGTCGGTGAAGAA
    AAGACTGTAGAAGAAATTATTACAGAGGTATTAAAAGACAAAGAATTTTACGAAGAATCC
    GGTGGAGGTTTAACTTTATCAGGAGGTGAAATATTTGCTCAGTTTGAATTTGCTAAAGCC
    ATCTTAAAATCAGCTAAAGAACATCACATACACACTGCCATTGAAACTACTGCCTTTGTT
    GATCATGAAAAATTTATTGATTTAATTCAATATGTGGATTTTATCTACACAGACCTAAAA
    CATTATAATTCTATAAAACATAAAAAAGTGACTGGGGTTTTTAATCAAATGATTATTAAA
    AACATTCATTATGCTTTTTCTCAAAATAAAACTATCGTTTTAAGAATCCCAGTTATTCCT
    AATTTTAACAATAGTTTAGAGGATGCAGAAAAATTCGCTACTCTATTTAACTCATTAAAT
    ATCGACCAAGTTCAACTACTCCCTTTTCATCAATTTGGTGAAAACAAATATCGTTTATTA
    AATCGGAAATATGAAATGGATGGAGTCAACGCACTTCATCCTGAAGATCTTATTGATTAT
    CAAAAGGTATTTCTGAACCACCATATTAATTGTTATTTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_257 [new locus tag: HKM25_RS01260 ]
  • symbol: HKM25_257
  • description: glycyl-radical enzyme activating family protein
  • length: 213
  • theoretical pI: 6.43771
  • theoretical MW: 24919.3
  • GRAVY: -0.370423

⊟Function[edit | edit source]

  • TIGRFAM:
    glycyl-radical enzyme activating protein (TIGR02494; EC 1.97.1.-; HMM-score: 203)
    Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 163.2)
    and 8 more
    Genetic information processing Protein fate Protein modification and repair pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 150.4)
    Metabolism Energy metabolism Anaerobic pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 150.4)
    Genetic information processing Protein fate Protein modification and repair [benzylsuccinate synthase]-activating enzyme (TIGR04003; EC 1.97.-.-; HMM-score: 136.6)
    Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 83.1)
    AmmeMemoRadiSam system radical SAM enzyme (TIGR04337; HMM-score: 22)
    Genetic information processing Protein synthesis tRNA and rRNA base modification putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03963; HMM-score: 17.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification wyosine biosynthesis protein TYW1 (TIGR03972; HMM-score: 17)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(2))-methyltransferase (TIGR00048; EC 2.1.1.192; HMM-score: 12.3)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 62.5)
    and 2 more
    4Fe-4S (CL0344) Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 24.1)
    Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 16.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9847
    • Cytoplasmic Membrane Score: 0.0056
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0095
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004728
    • TAT(Tat/SPI): 0.003915
    • LIPO(Sec/SPII): 0.000327
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: QJS35969 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEFAKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFNQMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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